Trivalent Disulfide Unit-Masked System Efficiently Delivers Large Oligonucleotide DOI Creative Commons
Lei Wang, Xiao Liu,

Yiliang Wu

и другие.

Molecules, Год журнала: 2024, Номер 29(17), С. 4223 - 4223

Опубликована: Сен. 5, 2024

Oligonucleotide drugs are shining in clinical therapeutics, but efficient and safe delivery systems severely limit their widespread use. A disulfide unit technology platform based on dynamic thiol exchange chemistry at the cell membrane has potential for drug delivery. However, alteration of CSSC dihedral angle induced by different substituents directly affects effectiveness this its stability. Previously, we constructed a trivalent low that can effectively promote cellular uptake small molecules. Here, novel unit-masked oligonucleotide hybrid unit, motivated prodrug design. Cellular imaging results showed such system exhibited superior efficiency than commercial Lipo2000 without cytotoxicity. The reagents significantly reduced (57–74%), which proved to be endocytosis-independent. In addition, vivo distribution experiments mice rapidly distributed liver tissues with duration action more 24 h, representing means silencing genes involved pathogenesis liver-like diseases. conclusion, deliver large drugs.

Язык: Английский

Screening and characterization of nucleic acid aptamers targeting Brucella surface antigens: identification and analysis of their corresponding binding sites DOI
Hao Wang, Bin Xie, Jianghua Yang

и другие.

World Journal of Microbiology and Biotechnology, Год журнала: 2025, Номер 41(6)

Опубликована: Июнь 1, 2025

Язык: Английский

Процитировано

0

Matrix Background Screening of an ssDNA Aptamer and Its Identification Against Lactopontin DOI Open Access
Chao Zhu,

Ziru Feng,

Mengmeng Yan

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(21), С. 11832 - 11832

Опубликована: Ноя. 4, 2024

Lactopontin (LPN) is a highly phosphorylated O-glycosylated acidic protein closely associated with infant gut, brain, and immune development, its recognition urgent due to rising application in fortified dairy products formula. In this study, an ssDNA aptamer against LPN was obtained, among which two kinds of matrix-background-assisted systematic evolution ligands via exponential enrichment (SELEX) approaches were performed compared. The direct approach utilize the sample matrix as mixing-incubation background between library that can theoretically increase screening pressure simulate practical scenarios. indirect PBS buffer include counter-screening steps adopt “sample matrix” whole target. Their evolutions monitored through qPCR assays from sequence diversity convergences each sub-library based on change proportion hetero- homo-duplexes dissociation curve melting temperature, also verified statistics high-throughput sequencing. common Seq.I1II3 finally fished out multiple analyses combining frequency, secondary structures, homology, binding assessments, demonstrated good specificity low-nanomolar affinity by assay (KD, 5.9 nM). addition, molecular docking dynamics simulation for their site prediction confirmation. This study provides potential identifying element basis accelerating development methods detection products.

Язык: Английский

Процитировано

1

State-of-the-Art Signal Amplification Strategies for Nucleic Acid and Non-Nucleic Acid Biosensors DOI Creative Commons
Ijaz Gul,

Muhammad Akmal Raheem,

Md. Reyad‐ul‐Ferdous

и другие.

Sensors and Actuators Reports, Год журнала: 2024, Номер unknown, С. 100268 - 100268

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

1

Overcoming Limited Access to Virus Infection Rapid Testing: Development of a Lateral Flow Test for SARS-CoV-2 with Locally Available Resources DOI Creative Commons

Estefanía S. Peri Ibáñez,

Agostina Mazzeo, Carolina Silva

и другие.

Biosensors, Год журнала: 2024, Номер 14(9), С. 416 - 416

Опубликована: Авг. 27, 2024

The COVID-19 pandemic highlighted testing inequities in developing countries. Lack of lateral flow test (LFT) manufacturing capacity was a major response bottleneck low- and middle-income regions. Here we report the development an open-access LFT for SARS-CoV-2 detection comparable to commercial tests that requires only locally available supplies. main critical resource is developed horse polyclonal antibody (pAb) whose sensitivity selectivity are greatly enhanced by affinity purification. We demonstrate these Abs can perform similarly monoclonal antibodies (mAbs), as well mAbs other pAbs against same antigen. workflow optimization using nasopharyngeal swabs collected RT-qPCR, spiked with inactivated virus determine analytical performance characteristics limit detection, among others. Our final prototype showed similar (sensitivity 83.3% compared 90.9% antigen tests). Finally, discuss possibility challenges utilizing affinity-purified alternative local outbreak context tool address inequalities access rapid tests.

Язык: Английский

Процитировано

0

Highly sensitive β-amyloid1-42 oligomer aptamer-based assay via dual cyclic amplifications of three-way catalytic hairpin assembly and palindrome polymerase reaction DOI

Zihao Yin,

Xiaoju Liu,

Shunmei Li

и другие.

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 423, С. 136750 - 136750

Опубликована: Окт. 9, 2024

Язык: Английский

Процитировано

0

Pattern recognition-based aptasensor array for discrimination of matrix effect DOI
Hao Liu,

Jiangsheng Mao,

Mengmeng Yan

и другие.

Microchemical Journal, Год журнала: 2024, Номер unknown, С. 111995 - 111995

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

0

The neutralisation and inhibitory effects of new tRNA-mimetic aptamer against cytotoxin-induced cytotoxicity in human skin keratinocytes DOI Creative Commons

Jia Jin Hiu,

Hock Siew Tan, Michelle Khai Khun Yap

и другие.

Biochimie, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Язык: Английский

Процитировано

0

Trivalent Disulfide Unit-Masked System Efficiently Delivers Large Oligonucleotide DOI Creative Commons
Lei Wang, Xiao Liu,

Yiliang Wu

и другие.

Molecules, Год журнала: 2024, Номер 29(17), С. 4223 - 4223

Опубликована: Сен. 5, 2024

Oligonucleotide drugs are shining in clinical therapeutics, but efficient and safe delivery systems severely limit their widespread use. A disulfide unit technology platform based on dynamic thiol exchange chemistry at the cell membrane has potential for drug delivery. However, alteration of CSSC dihedral angle induced by different substituents directly affects effectiveness this its stability. Previously, we constructed a trivalent low that can effectively promote cellular uptake small molecules. Here, novel unit-masked oligonucleotide hybrid unit, motivated prodrug design. Cellular imaging results showed such system exhibited superior efficiency than commercial Lipo2000 without cytotoxicity. The reagents significantly reduced (57–74%), which proved to be endocytosis-independent. In addition, vivo distribution experiments mice rapidly distributed liver tissues with duration action more 24 h, representing means silencing genes involved pathogenesis liver-like diseases. conclusion, deliver large drugs.

Язык: Английский

Процитировано

0