Molecules,
Год журнала:
2024,
Номер
29(17), С. 4223 - 4223
Опубликована: Сен. 5, 2024
Oligonucleotide
drugs
are
shining
in
clinical
therapeutics,
but
efficient
and
safe
delivery
systems
severely
limit
their
widespread
use.
A
disulfide
unit
technology
platform
based
on
dynamic
thiol
exchange
chemistry
at
the
cell
membrane
has
potential
for
drug
delivery.
However,
alteration
of
CSSC
dihedral
angle
induced
by
different
substituents
directly
affects
effectiveness
this
its
stability.
Previously,
we
constructed
a
trivalent
low
that
can
effectively
promote
cellular
uptake
small
molecules.
Here,
novel
unit-masked
oligonucleotide
hybrid
unit,
motivated
prodrug
design.
Cellular
imaging
results
showed
such
system
exhibited
superior
efficiency
than
commercial
Lipo2000
without
cytotoxicity.
The
reagents
significantly
reduced
(57–74%),
which
proved
to
be
endocytosis-independent.
In
addition,
vivo
distribution
experiments
mice
rapidly
distributed
liver
tissues
with
duration
action
more
24
h,
representing
means
silencing
genes
involved
pathogenesis
liver-like
diseases.
conclusion,
deliver
large
drugs.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(21), С. 11832 - 11832
Опубликована: Ноя. 4, 2024
Lactopontin
(LPN)
is
a
highly
phosphorylated
O-glycosylated
acidic
protein
closely
associated
with
infant
gut,
brain,
and
immune
development,
its
recognition
urgent
due
to
rising
application
in
fortified
dairy
products
formula.
In
this
study,
an
ssDNA
aptamer
against
LPN
was
obtained,
among
which
two
kinds
of
matrix-background-assisted
systematic
evolution
ligands
via
exponential
enrichment
(SELEX)
approaches
were
performed
compared.
The
direct
approach
utilize
the
sample
matrix
as
mixing-incubation
background
between
library
that
can
theoretically
increase
screening
pressure
simulate
practical
scenarios.
indirect
PBS
buffer
include
counter-screening
steps
adopt
“sample
matrix”
whole
target.
Their
evolutions
monitored
through
qPCR
assays
from
sequence
diversity
convergences
each
sub-library
based
on
change
proportion
hetero-
homo-duplexes
dissociation
curve
melting
temperature,
also
verified
statistics
high-throughput
sequencing.
common
Seq.I1II3
finally
fished
out
multiple
analyses
combining
frequency,
secondary
structures,
homology,
binding
assessments,
demonstrated
good
specificity
low-nanomolar
affinity
by
assay
(KD,
5.9
nM).
addition,
molecular
docking
dynamics
simulation
for
their
site
prediction
confirmation.
This
study
provides
potential
identifying
element
basis
accelerating
development
methods
detection
products.
Biosensors,
Год журнала:
2024,
Номер
14(9), С. 416 - 416
Опубликована: Авг. 27, 2024
The
COVID-19
pandemic
highlighted
testing
inequities
in
developing
countries.
Lack
of
lateral
flow
test
(LFT)
manufacturing
capacity
was
a
major
response
bottleneck
low-
and
middle-income
regions.
Here
we
report
the
development
an
open-access
LFT
for
SARS-CoV-2
detection
comparable
to
commercial
tests
that
requires
only
locally
available
supplies.
main
critical
resource
is
developed
horse
polyclonal
antibody
(pAb)
whose
sensitivity
selectivity
are
greatly
enhanced
by
affinity
purification.
We
demonstrate
these
Abs
can
perform
similarly
monoclonal
antibodies
(mAbs),
as
well
mAbs
other
pAbs
against
same
antigen.
workflow
optimization
using
nasopharyngeal
swabs
collected
RT-qPCR,
spiked
with
inactivated
virus
determine
analytical
performance
characteristics
limit
detection,
among
others.
Our
final
prototype
showed
similar
(sensitivity
83.3%
compared
90.9%
antigen
tests).
Finally,
discuss
possibility
challenges
utilizing
affinity-purified
alternative
local
outbreak
context
tool
address
inequalities
access
rapid
tests.
Molecules,
Год журнала:
2024,
Номер
29(17), С. 4223 - 4223
Опубликована: Сен. 5, 2024
Oligonucleotide
drugs
are
shining
in
clinical
therapeutics,
but
efficient
and
safe
delivery
systems
severely
limit
their
widespread
use.
A
disulfide
unit
technology
platform
based
on
dynamic
thiol
exchange
chemistry
at
the
cell
membrane
has
potential
for
drug
delivery.
However,
alteration
of
CSSC
dihedral
angle
induced
by
different
substituents
directly
affects
effectiveness
this
its
stability.
Previously,
we
constructed
a
trivalent
low
that
can
effectively
promote
cellular
uptake
small
molecules.
Here,
novel
unit-masked
oligonucleotide
hybrid
unit,
motivated
prodrug
design.
Cellular
imaging
results
showed
such
system
exhibited
superior
efficiency
than
commercial
Lipo2000
without
cytotoxicity.
The
reagents
significantly
reduced
(57–74%),
which
proved
to
be
endocytosis-independent.
In
addition,
vivo
distribution
experiments
mice
rapidly
distributed
liver
tissues
with
duration
action
more
24
h,
representing
means
silencing
genes
involved
pathogenesis
liver-like
diseases.
conclusion,
deliver
large
drugs.
