Analysis of Unique Motility of the Unicellular Green Alga Chlamydomonas reinhardtii at Low Temperatures down to −8 °C DOI Creative Commons
Kyôhei Yamashita,

Tomoka Yamaguchi,

S. Ikeno

и другие.

Micromachines, Год журнала: 2024, Номер 15(3), С. 410 - 410

Опубликована: Март 19, 2024

Previous studies of motility at low temperatures in Chlamydomonas reinhardtii have been conducted up to 15 °C. In this study, we report that C. exhibits unique a lower temperature range (−8.7 1.7 °C). Cell was recorded using four low-cost, easy-to-operate observation systems. Fast Fourier transform (FFT) analysis room (20–27 °C) showed the main peak frequency oscillations ranged from 44 61 Hz, which is consistent with 60 Hz beat flagella. At temperatures, swimming velocity decreased decreasing temperature. The results FFT major shifted 5–18 range, suggesting flagellar decreasing. spectra had distinct peaks both ranges, indicating were regular. This not affected by wavelength light source (white, red, green or blue LED) environmental spatial scale cells. contrast, cells highly viscous (3.5 mPa·s) culture numerous 0–200 band, irregular. These findings contribute better understanding under lower-temperature conditions reinhardtii.

Язык: Английский

Extracellular Vesicle-Like Nanoparticles, Artificially Created by Heat Treatment of Euglena gracilis, Exhibit Autofluorescence and Suppress IL-6 Expression in RAW264.7 Cells DOI

Ayumi Sakurai,

K. Egashira,

Rinka Kizaki

и другие.

BioNanoScience, Год журнала: 2025, Номер 15(2)

Опубликована: Март 17, 2025

Язык: Английский

Процитировано

0
Comparative Analysis of Pretreatment Methods for Fruit Waste Valorization in Euglena gracilis Cultivation: Impacts on Biomass, β-1,3-Glucan Production, and Photosynthetic Efficiency DOI Creative Commons
Jiangyu Zhu,

Xinyue Guo,

Kaile Zhao

и другие.

Foods, Год журнала: 2024, Номер 13(21), С. 3439 - 3439

Опубликована: Окт. 28, 2024

This study explored the sustainable valorization of fruit waste extracts from sugarcane bagasse (SB), banana peel (BP), and watermelon rind (WR) for Euglena gracilis biomass β-1,3-glucan production. The were prepared using water extraction (WE), high-temperature pressure treatment (HTP), dilute sulfuric acid (DSA). DSA-treated consistently yielded best results. E. cultured in SB-DSA showed highest cell density with a 2.08-fold increase compared to commercial HUT medium, followed by BP-DSA (1.35-fold) WR-DSA (1.70-fold). Photosynthetic pigment production increased significantly, chlorophyll yield being (1.90-fold increase). a/b ratio total carotenoid content also improved, indicating enhanced light-harvesting capacity photoprotection. efficiency, measured fluorescence, notably improved. maximum quantum PSII (Fv/Fm) up 25.88% SB-DSA, suggesting reduced stress improved overall photosynthetic health. potential photochemical efficiency (Fv/F0) even greater improvements: 40.53% SB-DSA. Cell morphology analysis revealed larger aspect ratios, implying more active cellular physiological state. 23.99%, 12.92%, 23.38% BP-DSA, WR-DSA, respectively. demonstrates pretreated as cost-effective medium cultivation, offering dual benefits high-value compound These findings contribute development efficient biorefinery processes align circular economy principles food biotechnology.

Язык: Английский

Процитировано

2
Reddening of the Unicellular Green Alga Euglena gracilis by Dried Bonito Stock and Intense Red Light Irradiation DOI Creative Commons
Kyôhei Yamashita, Ryusei Hanaki,

Ayaka Mori

и другие.

Plants, Год журнала: 2024, Номер 13(4), С. 510 - 510

Опубликована: Фев. 12, 2024

This study confirms for the first time that significant red coloration of Euglena gracilis is induced by bonito stock (BS), a traditional Japanese food, and intense light exposure (605~660 nm, 1000~1300 µmol photons/m2/s). Under condition, excessive photosynthetic activity destroyed many chloroplasts, while carotenoids were maintained, resulting in formation reddened cells. The HPLC analysis revealed diadinoxanthin was primary carotenoid present Additionally, an undefined xanthophyll, not produced under normal culture conditions, synthesized suggested to contain C=O bond. While it has been reported strong stress can increase total content cells, this did verify claim, should be investigated further future research. white irradiation conditions (90 μmol photons/m2/s) BS medium, no reddening cells observed, good growth achieved (over four times cell density CM medium on seventh day). suspension considered have high nutritional value because composed functional E. gracilis. fact method does involve genetic modification suggests possibility industrial applications, including food use, even

Язык: Английский

Процитировано

0
Analysis of Unique Motility of the Unicellular Green Alga Chlamydomonas reinhardtii at Low Temperatures down to −8 °C DOI Creative Commons
Kyôhei Yamashita,

Tomoka Yamaguchi,

S. Ikeno

и другие.

Micromachines, Год журнала: 2024, Номер 15(3), С. 410 - 410

Опубликована: Март 19, 2024

Previous studies of motility at low temperatures in Chlamydomonas reinhardtii have been conducted up to 15 °C. In this study, we report that C. exhibits unique a lower temperature range (−8.7 1.7 °C). Cell was recorded using four low-cost, easy-to-operate observation systems. Fast Fourier transform (FFT) analysis room (20–27 °C) showed the main peak frequency oscillations ranged from 44 61 Hz, which is consistent with 60 Hz beat flagella. At temperatures, swimming velocity decreased decreasing temperature. The results FFT major shifted 5–18 range, suggesting flagellar decreasing. spectra had distinct peaks both ranges, indicating were regular. This not affected by wavelength light source (white, red, green or blue LED) environmental spatial scale cells. contrast, cells highly viscous (3.5 mPa·s) culture numerous 0–200 band, irregular. These findings contribute better understanding under lower-temperature conditions reinhardtii.

Язык: Английский

Процитировано

0