Genomics and physiology of Catenibacillus, human gut bacteria capable of polyphenol C-deglycosylation and flavonoid degradation DOI Creative Commons
Tobias Goris, Annett Braune

Microbial Genomics, Год журнала: 2024, Номер 10(5)

Опубликована: Май 24, 2024

The genus Catenibacillus (family Lachnospiraceae , phylum Bacillota ) includes only one cultivated species so far, scindens, isolated from human faeces and capable of deglycosylating dietary polyphenols degrading flavonoid aglycones. Another intestinal strain not taxonomically resolved at that time was recently genome-sequenced. We analysed the genome this novel isolate, designated decagia showed its ability to deglycosylate C -coupled flavone xanthone glucosides O glycosides. Most resulting aglycones were further degraded corresponding phenolic acids. Including sequenced C. scindens ten faecal metagenome-assembled genomes assigned we performed a comparative analysis searched for genes encoding potential -glycosidases other polyphenol-converting enzymes. According data physiological characterization, core metabolism strains is based on fermentative lifestyle with butyrate production hydrogen evolution. Both encode -glycosidase, reductase, flavanone/flavanonol-cleaving reductase phloretin hydrolase. Several gene clusters enzymes similar those -deglycosylation system Dorea PUE (DgpBC), while separately located putative polyphenol-glucoside oxidases (DgpA) required -deglycosylation. diversity dgpA dgpBC might explain broad -glycoside substrate spectrum . few flavonoid-converting Our results indicate several are well-equipped degrade plant inhabit corresponding, specific niche in gut.

Язык: Английский

Complex Probiotics Suppress Inflammation by Regulating Intestinal Metabolites in Kittens DOI Creative Commons
Shimin Zhu, Musu Zha, Yanan Xia

и другие.

Animals, Год журнала: 2025, Номер 15(2), С. 272 - 272

Опубликована: Янв. 19, 2025

Cats are popular companions for humans, and their health is of importance to a growing number pet owners. The juvenile period critical stage cat growth; in particular, the metabolic activity intestinal microbiome kittens processing nutrients supporting overall health. In this study, effect complex probiotics on was explored through metabolomics analysis. Twenty-four healthy were randomly assigned two groups (n = 12): control group provided basal diet supplemented with (given at same time daily). acclimatized 5 days, experiment conducted 14 days. We collected feces from each kitten days 1 metabolomic analyses. Compared control, had significantly higher (p < 0.05) methylmalonylcarnitine, lysyl-hydroxyproline, phenylpropionylglycine, vitamin K3 levels, lower gamma-glutamyl-L-putrescine, cis-gondoic acid, myristic 12,13-DiHOME, glycodeoxycholic acid levels. results study suggest that promote by regulating changes various metabolites intestine may have mitigating inflammation.

Язык: Английский

Процитировано

1

Growth Characteristics of Peanut (Arachis hypogaea L. cv. Sinpalkwang) as Influenced by Irrigation Frequency and Temperature Conditions DOI
Juo Lee,

Jae Sung Jeon,

Hoon Seonwoo

и другие.

Journal of Biosystems Engineering, Год журнала: 2025, Номер unknown

Опубликована: Янв. 7, 2025

Язык: Английский

Процитировано

0

Moderate Highland Barley Intake Affects Anti-Fatigue Capacity in Mice via Metabolism, Anti-Oxidative Effects and Gut Microbiota DOI Open Access

Liangxing Zhao,

Qingyu Zhao, Sameh Sharafeldin

и другие.

Nutrients, Год журнала: 2025, Номер 17(4), С. 733 - 733

Опубликована: Фев. 19, 2025

this study aimed to explore the effects of different intake levels (20-80%) highland barley on anti-fatigue capacity ICR mice, focusing energy metabolism, metabolite accumulation, oxidative stress, and changes in gut microbiota. male mice were assigned five groups: control (normal diet) four experimental groups with supplementation at 20%, 40%, 60%, 80% total dietary energy. Anti-fatigue performance was assessed by behavioral experiments (rotarod, running, exhaustive swimming tests), biochemical markers, microbiota analysis. results showed that moderate (20%) significantly enhanced exercise endurance capacity, as evidenced increased liver glycogen (134.48%), muscle (87.75%), ATP content (92.07%), Na+-K+-ATPase activity (48.39%), antioxidant enzyme activities (superoxide dismutase (103.31%), catalase glutathione peroxidase (81.14%). Post-exercise accumulation blood lactate, quadriceps serum urea nitrogen, stress marker malondialdehyde reduced, differences 31.52%, 21.83%, 21.72%, 33.76%, respectively. Additionally, 20% promoted growth beneficial associated effects, including unclassified_f_Lachnospiraceae, g_norank_f_Peptococcaceae, Lachnospiraceae NK4A136, Colidextribacter, Turicibacter. However, when reached 60% or more, diminished, decreased activity, metabolic waste, a rise potentially harmful (Allobaculum, Desulfovibrio, norank_f_norank_o_RF39). (20% energy) enhances while excessive (≥60%) may have adverse effects.

Язык: Английский

Процитировано

0

Soybean Sprout Peptides Alleviate Obesity via PI3K-Akt and JAK-STAT Pathway Modulation, Gut Microbiota Regulation, and Metabolic Reprogramming DOI
Yi Wu,

Yilin Ling,

Yuqi Yang

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 7, 2025

Obesity is a growing global health concern associated with severe metabolic disorders, necessitating the development of safer and more effective therapeutic strategies. Soybean sprout peptides (SSPs), derived from germinated soybeans, are bioactive compounds potential antiobesity effects. This study aimed to investigate molecular mechanisms SSPs through an integrated approach combining network pharmacology, docking, in vivo experiments. SSP sequences were identified using UPLC-Orbitrap-MS/MS, their bioactivity was predicted PeptideRanker. Network pharmacology key targets, including AKT1, SRC, STAT3, ESR1, FOS, NFKB1, which implicated PI3K-Akt JAK-STAT pathways. Molecular docking validated strong interactions between these targets. In vivo, administration significantly reduced body weight gain, abdominal fat accumulation, serum lipid abnormalities high-fat-diet-induced obese mice while modulating gut microbiota composition by restoring Firmicutes-to-Bacteroidetes ratio reducing pathogenic taxa. Fecal metabolomics revealed that alleviated oxidative stress improved amino acid metabolism, contributing its These findings suggest holds promise as functional food ingredient or nutraceutical for obesity prevention management.

Язык: Английский

Процитировано

0

Genomics and physiology of Catenibacillus, human gut bacteria capable of polyphenol C-deglycosylation and flavonoid degradation DOI Creative Commons
Tobias Goris, Annett Braune

Microbial Genomics, Год журнала: 2024, Номер 10(5)

Опубликована: Май 24, 2024

The genus Catenibacillus (family Lachnospiraceae , phylum Bacillota ) includes only one cultivated species so far, scindens, isolated from human faeces and capable of deglycosylating dietary polyphenols degrading flavonoid aglycones. Another intestinal strain not taxonomically resolved at that time was recently genome-sequenced. We analysed the genome this novel isolate, designated decagia showed its ability to deglycosylate C -coupled flavone xanthone glucosides O glycosides. Most resulting aglycones were further degraded corresponding phenolic acids. Including sequenced C. scindens ten faecal metagenome-assembled genomes assigned we performed a comparative analysis searched for genes encoding potential -glycosidases other polyphenol-converting enzymes. According data physiological characterization, core metabolism strains is based on fermentative lifestyle with butyrate production hydrogen evolution. Both encode -glycosidase, reductase, flavanone/flavanonol-cleaving reductase phloretin hydrolase. Several gene clusters enzymes similar those -deglycosylation system Dorea PUE (DgpBC), while separately located putative polyphenol-glucoside oxidases (DgpA) required -deglycosylation. diversity dgpA dgpBC might explain broad -glycoside substrate spectrum . few flavonoid-converting Our results indicate several are well-equipped degrade plant inhabit corresponding, specific niche in gut.

Язык: Английский

Процитировано

2