Linking oxysterols and different stages of mild cognitive impairment: insights from gut metabolites and N6-methyladenosine DOI Creative Commons
Wenjing Feng, Mengwei Ju, Tao Wang

и другие.

Alzheimer s Research & Therapy, Год журнала: 2025, Номер 17(1)

Опубликована: Май 13, 2025

Oxysterols, gut metabolites, and N6-methyladenosine (m6A) are extensively implicated in the pathogenesis of cognitive dysfunction, while their alterations different stages mild impairment (MCI) have not been elucidated. Therefore, this study was conducted to explore associations oxysterols, m6A methylation profiles early MCI (EMCI) late (LMCI) individuals. Liquid chromatography-mass spectrometry, untargeted metabolomic analysis, mRNA Epitranscriptomic Microarray were used detect characteristics serum oxysterols (n = 35/group), fecal metabolites 30/group), whole blood 4/group) respectively. The concentration β-amyloid (Aβ) detected with ELISA 25/group). gene expression amyloid precursor protein (APP) its key enzyme β-secretase (BACE1) measured by quantitative real-time PCR EMCIs LMCIs, especially exhibited poorer performance almost all global multidimensional tests. Serum 27-hydroxycholesterol (27-OHC) 24S-hydroxycholesterol (24S-OHC) elevated EMCI LMCI groups. Changes occurred mainly group, which several including Procyanidin dimer B7 Phorbol myristate, significantly decreased. landscape LMCIs obviously differed from Controls. Hypomethylated mRNAs accounted for majority accompanied downregulated mRNAs, consistent writer methyltransferase-like 4 (METTL4). 27-OHC 24S-OHC combined various distinguished between subgroups healthy controls (EMCI/Control: AUC 0.877; LMCI/Control: 0.952). Heatmap revealed correlation myristate differentially m6A-methylated mRNAs. Differentially expressed methylated commonly enriched 34 KEGG metabolic pathways, cholesterol metabolism neurodegenerative disease-related pathways. Our explored altered MCI. potential function aberrant driving progression warrants further mechanistic investigation.

Язык: Английский

Recent advances in the extraction, purification and analytical techniques for flavonoids from plants: Taking hawthorn as an example DOI
Zhe Han, Chunfeng Li, Guiyan Liu

и другие.

Journal of Food Composition and Analysis, Год журнала: 2025, Номер unknown, С. 107372 - 107372

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

4

Natural Compounds Exert Anti‐Obesity Effects by Regulating Cytokines DOI

Yu Zhang,

Guize Feng, Weidong Zhang

и другие.

Phytotherapy Research, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

ABSTRACT Obesity, along with its associated health risks such as hypertension, hyperlipidemia, Type 2 diabetes, stroke, metabolic syndrome, asthma, and cancer, constitutes a significant global burden, contributing substantially to morbidity mortality. Cytokines, group of secreted signaling proteins, are crucial in initiating, maintaining, resolving immune responses. Although cytokines have unique advantages regulating functions, their therapeutic application for obesity remains limited clinical practice. Natural compounds, known structural diversity low toxicity, become valuable resource drug development. Many natural compounds shown anti‐obesity effects. This review comprehensively examines the mechanisms underlying obesity, specific focus on roles cytokines, inflammatory adipokines, growth factors. Additionally, it highlights regulatory interactions between gut microbiota obesity. The critically analyzes current pharmacological interventions summarizes advanced methodologies identifying potential compounds. Finally, identifies promising that modulate cytokine activity prevent or treat assesses complementary alternative therapies.

Язык: Английский

Процитировано

0

Effects of natural products on macrophage immunometabolism: a new frontier in the treatment of metabolic diseases DOI Creative Commons

Jiani Li,

Guo Chen, Xiaofei Yang

и другие.

Pharmacological Research, Год журнала: 2025, Номер unknown, С. 107634 - 107634

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Simultaneously phytochemical characteristic interpretation and efficient extraction of bioactive polar substances in Phragmites australis DOI
Yumei Wang,

GU Mei-ling,

Yong Chol Han

и другие.

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113880 - 113880

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0

Linking oxysterols and different stages of mild cognitive impairment: insights from gut metabolites and N6-methyladenosine DOI Creative Commons
Wenjing Feng, Mengwei Ju, Tao Wang

и другие.

Alzheimer s Research & Therapy, Год журнала: 2025, Номер 17(1)

Опубликована: Май 13, 2025

Oxysterols, gut metabolites, and N6-methyladenosine (m6A) are extensively implicated in the pathogenesis of cognitive dysfunction, while their alterations different stages mild impairment (MCI) have not been elucidated. Therefore, this study was conducted to explore associations oxysterols, m6A methylation profiles early MCI (EMCI) late (LMCI) individuals. Liquid chromatography-mass spectrometry, untargeted metabolomic analysis, mRNA Epitranscriptomic Microarray were used detect characteristics serum oxysterols (n = 35/group), fecal metabolites 30/group), whole blood 4/group) respectively. The concentration β-amyloid (Aβ) detected with ELISA 25/group). gene expression amyloid precursor protein (APP) its key enzyme β-secretase (BACE1) measured by quantitative real-time PCR EMCIs LMCIs, especially exhibited poorer performance almost all global multidimensional tests. Serum 27-hydroxycholesterol (27-OHC) 24S-hydroxycholesterol (24S-OHC) elevated EMCI LMCI groups. Changes occurred mainly group, which several including Procyanidin dimer B7 Phorbol myristate, significantly decreased. landscape LMCIs obviously differed from Controls. Hypomethylated mRNAs accounted for majority accompanied downregulated mRNAs, consistent writer methyltransferase-like 4 (METTL4). 27-OHC 24S-OHC combined various distinguished between subgroups healthy controls (EMCI/Control: AUC 0.877; LMCI/Control: 0.952). Heatmap revealed correlation myristate differentially m6A-methylated mRNAs. Differentially expressed methylated commonly enriched 34 KEGG metabolic pathways, cholesterol metabolism neurodegenerative disease-related pathways. Our explored altered MCI. potential function aberrant driving progression warrants further mechanistic investigation.

Язык: Английский

Процитировано

0