β-Nicotinamide Mononucleotide Reduces Oxidative Stress and Improves Steroidogenesis in Granulosa Cells Associated with Sheep Prolificacy via Activating AMPK Pathway DOI Creative Commons
Yu Cai, Hua Yang, Hui Xu

и другие.

Antioxidants, Год журнала: 2024, Номер 14(1), С. 34 - 34

Опубликована: Дек. 30, 2024

Oxidative stress is a significant factor in the death of granulosa cells (GCs), leading to follicular atresia and consequently limiting number dominant follicles that can mature ovulate within each wave. Follicular fluid contains diverse array metabolites play crucial roles regulating GCs’ proliferation oocyte maturation, which are essential for follicle development female fertility. However, mechanisms behind metabolite heterogeneity its effects on function remain poorly understood. Here, we identified elevated nicotinamide levels high-prolificacy sheep, correlated with oxidative GCs, by an integrated analysis. In vitro experiments demonstrated supplementation β-nicotinamide mononucleotide (NMN) significantly increased adenine dinucleotide (NAD+) adenosine triphosphate (ATP) GCs. NMN treatment effectively reduced Lipopolysaccharide (LPS)-induced apoptosis mitigated mitochondrial dysfunction, while also decreasing production reactive oxygen species (ROS), thereby enhancing activity antioxidant defense system. Importantly, improved impairments steroid hormone induced LPS. Mechanistically, protective against GCs were mediated via AMPK/mTOR pathway. Collectively, our findings elucidate metabolic characteristics associated sheep prolificacy demonstrate protects from LPS-induced dysfunction enhances ovarian responsiveness These position as potential novel biomarker function.

Язык: Английский

Nicotinamide mononucleotide combined with PJ-34 protects microglial cells from lipopolysaccharide-induced mitochondrial impairment through NMNAT3-PARP1 axis DOI Creative Commons
Lee Jia, Xiao-Yu Cheng, Ruixia Ma

и другие.

Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)

Опубликована: Март 6, 2025

Lipopolysaccharide (LPS) is known to induce cell injury and mitochondrial dysfunction, which are pivotal in neuroinflammation related disorders. Recent studies have demonstrated the potential of nicotinamide mononucleotide (NMN) poly(ADP-ribose) polymerase-1 (PARP1) inhibitors enhance function. However, underlying mechanisms not been fully elucidated. This study investigates impact NMN conjunction with PJ-34, a PARP1 inhibitor, on LPS-induced damage, focusing adenylyl transferase 3 (NMNAT3) -PARP1 axis. The results showed that LPS treatment led down-regulation NMNAT3 (decreased 58.72% at 1 µM), up-regulation (enhanced 22.78% thereby impairing mitophagy negative effects can be mitigated through supplementation PJ-34. Specifically, compared group, expression increased by 63.29% decreased 27.94% concentration 400 µM NMN. Additionally, when was combined 5 21.99%. Mechanistic reveal PJ-34 counteracted detrimental promoting binding FoxO1 PINK1 promoter activate PINK1/Parkin mediated pathway. Further experimental demonstrate inhibit initiation autophagic processes. Consequently, targeting NMNAT3-PARP1 signaling pathway holds promise for development novel therapeutic strategies alleviate damage-related

Язык: Английский

Процитировано

0
Trends in intestinal aging: From underlying mechanisms to therapeutic strategies DOI Creative Commons
Yajun Wang, Xueni Zhang,

Mengli Qing

и другие.

Acta Pharmaceutica Sinica B, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
β-Nicotinamide Mononucleotide Reduces Oxidative Stress and Improves Steroidogenesis in Granulosa Cells Associated with Sheep Prolificacy via Activating AMPK Pathway DOI Creative Commons
Yu Cai, Hua Yang, Hui Xu

и другие.

Antioxidants, Год журнала: 2024, Номер 14(1), С. 34 - 34

Опубликована: Дек. 30, 2024

Oxidative stress is a significant factor in the death of granulosa cells (GCs), leading to follicular atresia and consequently limiting number dominant follicles that can mature ovulate within each wave. Follicular fluid contains diverse array metabolites play crucial roles regulating GCs’ proliferation oocyte maturation, which are essential for follicle development female fertility. However, mechanisms behind metabolite heterogeneity its effects on function remain poorly understood. Here, we identified elevated nicotinamide levels high-prolificacy sheep, correlated with oxidative GCs, by an integrated analysis. In vitro experiments demonstrated supplementation β-nicotinamide mononucleotide (NMN) significantly increased adenine dinucleotide (NAD+) adenosine triphosphate (ATP) GCs. NMN treatment effectively reduced Lipopolysaccharide (LPS)-induced apoptosis mitigated mitochondrial dysfunction, while also decreasing production reactive oxygen species (ROS), thereby enhancing activity antioxidant defense system. Importantly, improved impairments steroid hormone induced LPS. Mechanistically, protective against GCs were mediated via AMPK/mTOR pathway. Collectively, our findings elucidate metabolic characteristics associated sheep prolificacy demonstrate protects from LPS-induced dysfunction enhances ovarian responsiveness These position as potential novel biomarker function.

Язык: Английский

Процитировано

0