Autonomous Nucleic Acid and Protein Nanocomputing Agents Engineered to Operate in Living Cells

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Янв. 6, 2025

In recent years, the rapid development and employment of autonomous technology have been observed in many areas human activity. Autonomous can readily adjust its function to environmental conditions enable an efficient operation without control. While applying same concept designing advanced biomolecular therapies would revolutionize nanomedicine, design approaches engineering biological nanocomputing agents for predefined operations within living cells remain a challenge. made nucleic acids proteins are appealing idea, two decades research has shown that engineered act under real physical biochemical constraints logical manner. Throughout all domains life, perform variety vital functions, where sequence-defined structures these biopolymers either operate on their own or efficiently together. This programmability synergy inspire massive efforts utilize versatility amino encode functions properties otherwise do not exist nature. Perspective covers key concepts used application discusses potential limitations paths forward.

Язык: Английский

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Living cells and biological mechanisms as prototypes for developing chemical artificial intelligence

Biochemical and Biophysical Research Communications, Год журнала: 2024, Номер 720, С. 150060 - 150060

Опубликована: Май 8, 2024

Artificial Intelligence (AI) is having a revolutionary impact on our societies. It helping humans in facing the global challenges of this century. Traditionally, AI developed software or through neuromorphic engineering hardware. More recently, brand-new strategy has been proposed. so-called Chemical (CAI), which exploits molecular, supramolecular, and systems chemistry wetware to mimic human intelligence. In work, two promising approaches for boosting CAI are described. One regards designing implementing neural surrogates that can communicate optical chemical signals give rise networks computational purposes develop micro/nanorobotics. The other approach concerns "bottom-up synthetic cells" be exploited applications various scenarios, including future nano-medicine. Both topics presented at basic level, mainly inform broader audience non-specialists, so favour interest these frontier subjects.

Язык: Английский

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Microfluidic technologies for lipid vesicle generation

Lab on a Chip, Год журнала: 2024, Номер 24(20), С. 4679 - 4716

Опубликована: Янв. 1, 2024

Encapsulating molecular materials in lipid vesicles via microfluidics provides control over size, morphology, and compartmentalisation, enabling their use cell biology research for applications biomedicine, synthetic biology, beyond.

Язык: Английский

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Switchable and orthogonal gene expression control inside artificial cells by synthetic riboswitches

Chemical Communications, Год журнала: 2024, Номер 60(46), С. 5972 - 5975

Опубликована: Янв. 1, 2024

Here we report two novel synthetic riboswitches that respond to ASP2905 and theophylline function in reconstituted cell-free protein synthesis (CFPS) system. We encapsulated the CFPS system as well DNA-templated encoding reporter genes regulated by these orthogonal inside liposomes, achieved switchable control over gene expression external stimulation with cognate ligands.

Язык: Английский

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On-the-Fly Microfluidic Control of Giant Vesicle Compositions Validated by DNA Surface Charge Sensors

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Апрель 4, 2025

The specific lipid composition of cell membrane microenvironments plays a critical role in regulating range cellular processes such as integral and peripheral protein function, morphology, potential. However, harnessing similar complex capabilities artificial mimics remains challenging. In large part, progress has been slow due to scarcity techniques for both (i) accurately quantifying composition-dependent properties models at the single-vesicle level (ii) efficiently exploring multidimensional spaces. Here, we address challenges by first developing an assay quantitatively sensing giant unilamellar vesicle (GUV) surface potentials using fluorescent cholesterol-labeled DNA duplex sensor. We then devised microfluidic assembly line enabling continuous, on-chip production vesicles with variable compositions. This enabled real-time, on-the-fly adjustment compositions biophysical were being produced, followed analysis our assay. Analysis association probe single reveals that may quantify potential membranes situ through quantification membrane-probe binding constant. Our work paves way libraries can enable rational engineering.

Язык: Английский

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Structural stability of wireframe DNA origami: The role of nanocomponent modifications

The Journal of Chemical Physics, Год журнала: 2025, Номер 162(15)

Опубликована: Апрель 15, 2025

Wireframe DNA origami nanostructures hold immense potential for diverse applications in nanotechnology. The design of wireframe structures traditionally follows a top–down approach. This study introduces complementary bottom–up approach to investigate the nano-components constituting these and their impact on structural stability. To this end, modifications edge staple crossovers, poly-T bulges, sequences were examined through coarse-grained molecular dynamics. results reveal that reducing number crossovers slightly alters distance between two double-stranded helices forming edges but maintains adequate removal however, leads opening under specific thermal conditions, whereas containing bulges remain intact, highlighting critical role Furthermore, changes sequences, achieved by repositioning scaffold nick, showed negligible effects overall stability structures. incorporation designing can enable creation with tailored properties applications. These be adapted variety structures, broadening uses

Язык: Английский

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Reconstitution of nanopores in bilayers of diblock and triblock copolymers

Physical Chemistry Chemical Physics, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Four amphiphilic polymers formed synthetic bilayers via the droplet-contact method. We found that monolayer adhesive energy is a key factor for membrane stability. These findings will improve durability of nanopore measurements.

Язык: Английский

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Nanopore‐Based Single‐Molecule Logic Unit (sMOLU)

Small, Год журнала: 2025, Номер unknown

Опубликована: Июнь 4, 2025

Abstract DNA computing has recently advanced from theoretical computations to a wide variety of applications. Single‐molecule combined with nanopore technology offers unique approach real‐time molecular computation. A single‐molecule logic unit (sMOLU) is developed that uses α‐hemolysin nanopores implement DNA‐based AND gate within lipid bilayers. This system leverages three‐way junction immobilized on membrane, enabling enzyme‐specific cleavage and signal amplification in giant unilamellar vesicles (GUVs). Fluorescence electrophysiological measurements confirm precise operations at the level. The sMOLU platform demonstrates potential for advancing nanoscale computational systems analysis capability by integrating technologies.

Язык: Английский

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Synthetic DNA nanopores for direct molecular transmission between lipid vesicles

Nanoscale, Год журнала: 2024, Номер 16(25), С. 12174 - 12183

Опубликована: Янв. 1, 2024

We designed a pair of DNA nanopores that can connect and form direct molecular pathway between lipid vesicles.

Язык: Английский

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Artificial Molecular Systems for Complex Functions Based on DNA Nanotechnology and Cell‐Sized Lipid Vesicles

ChemSystemsChem, Год журнала: 2024, Номер 6(4)

Опубликована: Май 11, 2024

Abstract Cells are highly functional and complex molecular systems. Artificially creating such systems remains a challenge, which has been extensively studied in various research fields, including synthetic biology robotics. DNA nanotechnology is powerful tool for bottom‐up engineering constructing nanostructures or chemical reaction networks can be utilized as components artificial Encapsulation of these into giant unilamellar vesicle (GUV) composed lipid bilayer, the base structure cellular membrane, results cell‐sized that partially mimics some functions. This review discusses studies contributing to construction GUV‐based based on nanotechnology. Molecular transport signal transduction through membranes essential uptake molecules from environment respond stimuli. Membrane shaping relates functions, motility signaling. A network required autonomously regulate system‘s describes functions realized using networks. Given designability programmability nanotechnology, it may possible functionality could comparable even surpass natural

Язык: Английский

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