Antibody-Functionalized Gold Nanoparticles as a Highly Sensitive Two-Step Colorimetric Biosensor for Detecting Salmonella Typhimurium in Food DOI

Mario Anzevino,

Daniele Marra, Andrea Fulgione

и другие.

ACS Applied Nano Materials, Год журнала: 2024, Номер 7(17), С. 21048 - 21056

Опубликована: Авг. 27, 2024

The standard laboratory methods used to detect Salmonella are time-consuming, leading unavoidable delays, which can be costly (e.g., food checks at customs) or even dangerous when quick alarms needed for potential outbreaks). To address this problem, we propose a rapid two-step colorimetric immunoassay. In the first step, gold nanoparticles interact with sample, while in second an optimized amount of saline solution is added induce cluster formation among unbound nanoparticles, providing color change that inversely correlates bacteria concentration. were functionalized using technique based on UV activation antibodies, resulting oriented antibodies antigen-binding regions proximity surface. While observed naked eye relatively higher bacterial concentrations, more quantitative approach UV–visible spectrum measurements allowed us achieve limit detection 1 cfu/mL chicken samples. test completed 15 min and performed on-site, paving way widespread applications our biosensor field safety.

Язык: Английский

Rapid and simple on-site salmonella detection in food via direct sample loading using a lipopolysaccharide-imprinted polymer DOI Creative Commons

Solpa Lee,

Hyunsoo Kim, Minwoo Kim

и другие.

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Апрель 7, 2025

Salmonella is a major foodborne pathogen that causes salmonellosis, which characterized by symptoms such as diarrhea, fever, and abdominal cramps. Existing methods for detecting Salmonella, culture plating, ELISA, PCR, are accurate but time-consuming unsuitable on-site applications. In this study, we developed rapid sensitive electrochemical sensor using molecularly imprinted polymer (MIP) to detect typhimurium (S. typhimurium) targeting lipopolysaccharides (LPS). Polydopamine (PDA) was used the matrix because of its cost-efficiency functional versatility. The demonstrated high sensitivity selectivity, with detection limit 10 CFU/mL linear response over 10²-10⁸ range. specificity validated against other gram-positive gram-negative bacteria showed no significant cross-reactivity. Furthermore, performed effectively in real food samples, including tap water, milk, pork, without complex preprocessing. These results highlight potential LPS-imprinted MIP practical S. typhimurium, improving safety monitoring preventing outbreaks food-handling environments.

Язык: Английский

Процитировано

0
Harnessing polydiacetylene (PDA): A review of structural mechanics and infectious disease detection DOI
Soumendu Patra, Harshita Shand, Sayan Ghosal

и другие.

Next Materials, Год журнала: 2025, Номер 8, С. 100687 - 100687

Опубликована: Апрель 28, 2025

Язык: Английский

Процитировано

0
A simple and efficient liposome-based biosensor for visual monitoring of Cu2+-bipyridine complex formation DOI
Vy Anh Tran,

Thu Thao Thi Vo,

Sang–Wha Lee

и другие.

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113982 - 113982

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Machine learning-enabled colorimetric sensors for foodborne pathogen detection DOI
Emma Holliday, Boce Zhang

Advances in food and nutrition research, Год журнала: 2024, Номер unknown, С. 179 - 213

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

2
Development of Novel DNA Aptamers and Colorimetric Nanozyme Aptasensor for Targeting Multi-Drug-Resistant, invasive Salmonella typhimurium Strain SMC25 DOI Creative Commons
Akanksha Joshi, Abhishek Kaushik, Neetu Kumra Taneja

и другие.

Biosensors and Bioelectronics X, Год журнала: 2024, Номер 21, С. 100555 - 100555

Опубликована: Окт. 24, 2024

Язык: Английский

Процитировано

0
Antibody-Functionalized Gold Nanoparticles as a Highly Sensitive Two-Step Colorimetric Biosensor for Detecting Salmonella Typhimurium in Food DOI

Mario Anzevino,

Daniele Marra, Andrea Fulgione

и другие.

ACS Applied Nano Materials, Год журнала: 2024, Номер 7(17), С. 21048 - 21056

Опубликована: Авг. 27, 2024

The standard laboratory methods used to detect Salmonella are time-consuming, leading unavoidable delays, which can be costly (e.g., food checks at customs) or even dangerous when quick alarms needed for potential outbreaks). To address this problem, we propose a rapid two-step colorimetric immunoassay. In the first step, gold nanoparticles interact with sample, while in second an optimized amount of saline solution is added induce cluster formation among unbound nanoparticles, providing color change that inversely correlates bacteria concentration. were functionalized using technique based on UV activation antibodies, resulting oriented antibodies antigen-binding regions proximity surface. While observed naked eye relatively higher bacterial concentrations, more quantitative approach UV–visible spectrum measurements allowed us achieve limit detection 1 cfu/mL chicken samples. test completed 15 min and performed on-site, paving way widespread applications our biosensor field safety.

Язык: Английский

Процитировано

0