Algae,
despite
being
labeled
as
an
underexplored
biological
source
of
chemical
constituents,
remain
inadequately
studied
in
terms
their
metabolism.
Metabolomics
has
emerged
a
high-throughput
technology
to
investigate
the
full
metabolic
profile
samples
that
could
aid
understanding
and
characterization
algae.
By
delving
into
primary
composition,
particularly
polysaccharides
phycobiliproteins,
alongside
secondary
metabolites
like
polyphenols
pigments,
researchers
can
uncover
not
only
rheological
nutritional
properties
but
also
diverse
activities.
Given
growing
interest
algae
food
related
industries,
innovative
approaches
should
be
explored
enhance
value
functional
components.
In
this
sense,
context
contemporary
in-silico
studies,
metabolomics
paired
with
computational
methodologies,
develop
novel
techniques
for
studying
biomolecular
interactions.
Molecular
docking
emerged,
function
predicting
atomic-level
interaction
between
small
molecules
(ligands)
target
proteins
(proteins).
This
synergistic
approach
integrating
both
technologies
allow
us
characterize
algal
profiles,
evaluate
potential
bioactive
properties,
better
understand
work
explores
development
metabolomic
strategies
targeted
toward
harnessing
these
technologies,
we
unlock
new
possibilities
using
various
industrial
applications,
paving
way
sustainable
solutions
future.
Advances in chemical and materials engineering book series,
Год журнала:
2025,
Номер
unknown, С. 151 - 170
Опубликована: Фев. 14, 2025
Metabolomic
analysis
presents
a
major
challenge
in
achieving
unambiguous
characterization
of
metabolites
detected
samples.
Among
the
various
techniques
used
metabolomics,
Nuclear
Magnetic
Resonance
(NMR)
spectroscopy
stands
out
for
its
ability
to
provide
detailed
structural
information.
When
integrated
with
other
spectroscopic
data,
this
information
facilitates
elucidation
complex
NMR
spectroscopy,
which
is
highly
sophisticated
and
powerful
tool
pharmaceutical
research,
playing
crucial
part
drug
discovery.
The
advent
heteronuclear
has
particularly
revolutionized
field
discovery
development.
This
chapter
addresses
practical
aspects
acquiring
standard
one-dimensional
(1D)
spectra
introduces
methods
distinguishing
known
undescribed
molecules
rapidly.
Furthermore,
it
delves
into
two-dimensional
(2D)
sequences,
such
as
1H-13C,
illustrates
how
these
combined
can
be
determine
structures
natural
products.
Journal of Chromatography Open,
Год журнала:
2024,
Номер
6, С. 100154 - 100154
Опубликована: Июль 17, 2024
The
chemoselective
probe
technique
combines
the
benefits
of
chemical
derivatization
and
solid-phase
extraction,
allowing
for
simultaneous
enhancement
analyte
ionization
efficiency,
reduction
matrix
interference,
improvement
in
chromatographic
separation.
This
innovative
holds
great
promise
applications
metabolomics
research,
metabolic
tracking,
natural
product
isolation
purification.
review
provides
a
comprehensive
overview
advancements
probes
from
2007
to
2024,
focusing
on
progress
design,
particularly
cleavable
linkers.
Additionally,
it
discusses
application
LC-MS
analysis
small-molecular-weight
compounds.
Current Opinion in Plant Biology,
Год журнала:
2024,
Номер
80, С. 102554 - 102554
Опубликована: Май 30, 2024
Elucidating
the
biochemical
pathways
of
specialised
metabolites
in
plants
is
key
to
enable
or
improve
their
sustainable
biotechnological
production.
Chemical
tools
can
greatly
facilitate
discovery
biosynthetic
genes
and
enzymes.
Here,
we
summarise
transdisciplinary
approaches
where
methods
from
chemistry
chemical
biology
helped
overcome
challenges
pathway
elucidation.
Based
on
recent
examples,
describe
how
state-of-the-art
isotope
labelling
experiments
guide
selection
gene
candidates,
affinity-based
probes
identification
novel
enzymes,
semisynthesis
availability
elusive
intermediates,
biomimetic
reactions
provide
a
better
understanding
inherent
reactivity.
We
anticipate
that
wider
application
such
will
accelerate
pace
elucidation
plants.
Abstract
Identification
of
therapeutic
targets
can
directly
elucidate
the
mechanism
and
effect
drug
therapy,
which
is
a
central
step
in
development.
The
disconnect
between
protein
phenotypes
under
complex
mechanisms
hampers
comprehensive
target
understanding.
Metabolomics,
as
systems
biology
tool
that
captures
phenotypic
changes
induced
by
exogenous
compounds,
has
emerged
valuable
approach
for
identification.
A
overview
was
provided
this
review
to
illustrate
principles
advantages
metabolomics,
delving
into
application
metabolomics
This
outlines
various
metabolomics‐based
methods,
such
dose–response
stable
isotope‐resolved
multiomics,
identify
key
enzymes
metabolic
pathways
affected
substances
through
dose‐dependent
metabolite–drug
interactions.
Emerging
techniques,
including
single‐cell
artificial
intelligence,
mass
spectrometry
imaging,
are
also
explored
their
potential
enhance
discovery.
emphasizes
metabolomics'
critical
role
advancing
our
understanding
disease
accelerating
targeted
development,
while
acknowledging
current
challenges
field.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 30, 2024
Abstract
Cryoconite
holes
are
biological
hotspots
with
a
high
biogeochemical
turnover
rate,
contributing
significantly
to
the
glacial
ecosystem’s
overall
carbon
cycles
and
net
fluxes.
Unfortunately,
information
about
composition
of
low
molecular
weight
molecules
formed
through
metabolic
processes
cryoconite-dwelling
microbes
is
scanty.
These
constitute
substantial
portion
dissolved
organic
matter
(DOM)
within
cryoconite
holes.
The
present
study
investigated
DOM
in
using
reverse-phase
liquid
chromatography
(RP-LC)
coupled
high-resolution
tandem
mass
spectrometry.
We
evaluated
various
solvent
combinations
water,
methanol,
acetonitrile
extract
chemically
diverse
polar
non-polar
metabolites
from
Among
single
solvents,
organic-rich
MeOH:
Water
(70:30
v/v)
parallel
2-single
Acetonitrile:
Methanol:
(40:40:20
provided
increased
number
chemical
diversity
extracted
metabolites.
Combining
RP
hydrophilic
interaction
(HILIC)
technique
highest
unique
This
dual-LC
ionization
polarity
combination
detection
features
by
46.96%
24.52%
single-
two-solvent
compared
alone.
developed
simple
untargeted
metabolomics
workflow
that
highly
sensitive
robust,
detecting
potentially
identifying
large
(extracellular)
(intracellular)
environment.
method
can
better
characterize
DOM’s
and,
after
integrating
other
‘omics’
approaches,
be
used
examine
link
between
pathways
microbial
communities
global
or
similar
ecosystems,
revealing
how
these
earthy
systems
their
flora
control
nutrient
storage
release
response
climate
change.
Overall,
presents
valuable
methodology
for
studying
biogeochemistry
Pharmacological Research,
Год журнала:
2024,
Номер
209, С. 107449 - 107449
Опубликована: Окт. 4, 2024
Cancer
treatment
remains
a
formidable
challenge
in
modern
medicine,
necessitating
nuanced
understanding
of
its
molecular
underpinnings
and
the
identification
novel
therapeutic
modalities.
Among
intricate
web
cellular
pathways
implicated
oncogenesis,
protein
synthesis
has
emerged
as
fundamental
process
warranting
meticulous
investigation.
This
review
elucidates
multifaceted
role
tumor
initiation
progression,
highlighting
potential
targeting
key
nodes
within
these
viable
strategies.
Natural
products
have
long
served
source
bioactive
compounds
with
owing
to
their
structural
diversity
evolutionary
honing.
Within
this
framework,
we
provide
thorough
examination
natural
inhibitors
promising
candidates
for
cancer
therapy,
drawing
upon
recent
advancements
mechanistic
insights.
By
synthesizing
current
evidence
elucidating
challenges
opportunities,
aims
galvanize
further
research
into
development
product-based
anticancer
therapeutics,
thereby
advancing
clinical
armamentarium
against
malignancies.
