Visualizing Single V-ATPase Rotation Using Janus Nanoparticles DOI
A. Otomo, Jared T. Wiemann,

Swagata Bhattacharyya

и другие.

Nano Letters, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 22, 2024

Understanding the function of rotary molecular motors, such as ATPases, relies on our ability to visualize single-molecule rotation. Traditional imaging methods often involve tagging those motors with nanoparticles (NPs) and inferring their rotation from translational motion NPs. Here, we report an approach using "two-faced" Janus NPs directly image a single V-ATPase Enterococcus hirae, ATP-driven ion pump. By employing 500 nm silica/gold NP, exploit its asymmetric optical contrast, silica core gold cap one hemisphere, achieve precise unidirectional counterclockwise immobilized surfaces. Despite added viscous load relatively large NP probe, provides accurate torque measurements V-ATPase. This study underscores advantages over conventional probes, establishing them powerful tools for analysis motors.

Язык: Английский

Dielectrophoretic Polarizability of Surface‐Modified Particles for Studying Induced‐Charge Electroosmotic Flows DOI Open Access
Ana Rita C. Duarte, Cooper P. Thome,

Wren S. Hoertdoerfer

и другие.

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Abstract Induced‐charge electroosmosis (ICEO) offers a practical approach to drive microscale flows by application of AC electric fields across polarizable surfaces, enabling diverse functions including microfluidic pumping, active cargo transport, and biosensing. While ICEO along pristine surfaces is well‐understood, applications often require surface modifications that affect in manner poorly understood. Here, this study introduces dielectrophoretic (DEP) polarizability measurement, DPM, as method effects on flows. The entails DEP trapping probe particles analysis their equilibrium motions measure polarizability. This DPM‐generated data then used predict reveal the contribution additional factors affecting ICEO. It compares predictions with experimentally observed changes speed Janus traveling ICEO‐driven induced‐charge electrophoresis. shows DPM enables prediction decreased particle upon protein capture functional reveals increased polymer‐modified likely arises from hydrodynamic factors. Overall, work lays foundation for investigating new systems complex environments, potentially those encountered biosensing, remediation, or delivery.

Язык: Английский

Процитировано

0
Diffusiophoresis: a novel transport mechanism - fundamentals, applications, and future opportunities DOI Creative Commons
Arkava Ganguly, Benjamin M. Alessio, Ankur Gupta

и другие.

Frontiers in Sensors, Год журнала: 2023, Номер 4

Опубликована: Ноя. 30, 2023

Diffusiophoresis involves the movement of colloidal-scale entities in response to concentration gradients a solute. It is broadly categorized into two types: passive and active diffusiophoresis. In diffusiophoresis, external drive motion, while colloidal entity itself assists generating gradients. this perspective, we delve fundamental processes underlying diffusiophoresis emphasize how prevalent both kinds are natural systems. particular, highlight focusing feature discuss it underpins variety experimental observations applications such as low-cost zetasizers, water filtration, biological pattern formation. For dependence particle trajectory on its shape surface heterogeneity, dictates drug delivery, removal microplastics, self-repairing materials. Finally, offer insights ideas regarding future opportunities

Язык: Английский

Процитировано

7
Current status and future application of electrically controlled micro/nanorobots in biomedicine DOI Creative Commons
Ruochen Pu,

Xiyu Yang,

Haoran Mu

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12

Опубликована: Янв. 19, 2024

Using micro/nanorobots (MNRs) for targeted therapy within the human body is an emerging research direction in biomedical science. These nanoscale to microscale miniature robots possess specificity and precision that are lacking most traditional treatment modalities. Currently, on electrically controlled still its early stages, with researchers primarily focusing fabrication manipulation of these meet complex clinical demands. This review aims compare fabrication, powering, locomotion various micro/nanorobots, explore their advantages, disadvantages, potential applications.

Язык: Английский

Процитировано

2
Unified mobility expressions for externally driven and self-phoretic propulsion of particles DOI
Arkava Ganguly, Souradeep Roychowdhury, Ankur Gupta

и другие.

Journal of Fluid Mechanics, Год журнала: 2024, Номер 994

Опубликована: Сен. 10, 2024

The mobility of externally driven phoretic propulsion particles is evaluated by simultaneously solving the solute conservation equation, interaction potential equation and modified Stokes equation. While accurate, this approach cumbersome, especially when decays slowly compared with particle size. In contrast to external phoresis, motion self-phoretic typically estimated relating translation rotation velocities local slip velocity. convenient thus widely used, it only valid decay length significantly smaller than Here, taking inspiration from Brady ( J. Fluid Mech. , vol. 922, 2021, A10), which combines benefits two approaches, we reproduce their unified expressions arbitrary potentials show that these can conveniently recover well-known relationships electrophoresis diffusiophoresis for double-layer thickness. Additionally, a spherical microswimmer, derived relax velocity calculations in limit thin scales. We also employ calculate an autophoretic Janus particle. find there significant dampening even order magnitude larger Finally, study catalytically self-propelled particle, while propels due concentration gradients, demonstrate how modes compete each other.

Язык: Английский

Процитировано

1
Visualizing Single V-ATPase Rotation Using Janus Nanoparticles DOI Creative Commons
A. Otomo, Jared T. Wiemann,

Swagata Bhattacharyya

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 22, 2024

Understanding the function of rotary molecular motors, such as ATPases, relies on our ability to visualize single-molecule rotation. Traditional imaging methods often involve tagging those motors with nanoparticles (NPs) and inferring their rotation from translational motion NPs. Here, we report an approach using ″two-faced″ Janus NPs directly image single V-ATPase Enterococcus hirae , ATP-driven ion pump. By employing a 500-nm silica/gold NP, exploit its asymmetric optical contrast — silica core gold cap one hemisphere achieve precise unidirectional counter-clockwise immobilized surfaces. Despite added viscous load relatively large NP probe, provides accurate torque measurements V-ATPase. This study underscores advantages over conventional probes, establishing them powerful tools for analysis motors.

Язык: Английский

Процитировано

0
Visualizing Single V-ATPase Rotation Using Janus Nanoparticles DOI
A. Otomo, Jared T. Wiemann,

Swagata Bhattacharyya

и другие.

Nano Letters, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 22, 2024

Understanding the function of rotary molecular motors, such as ATPases, relies on our ability to visualize single-molecule rotation. Traditional imaging methods often involve tagging those motors with nanoparticles (NPs) and inferring their rotation from translational motion NPs. Here, we report an approach using "two-faced" Janus NPs directly image a single V-ATPase Enterococcus hirae, ATP-driven ion pump. By employing 500 nm silica/gold NP, exploit its asymmetric optical contrast, silica core gold cap one hemisphere, achieve precise unidirectional counterclockwise immobilized surfaces. Despite added viscous load relatively large NP probe, provides accurate torque measurements V-ATPase. This study underscores advantages over conventional probes, establishing them powerful tools for analysis motors.

Язык: Английский

Процитировано

0