Enzyme Bioink for the 3D Printing of Biocatalytic Materials DOI Creative Commons
Luca A. Altevogt, Rakib Sheikh, Thomas G. Molley

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 5, 2024

Abstract The field of 3D biofabrication faces major challenges on the road to printing fully functional tissues and organs. One them is adding functionality newly formed tissue for replicating an active biochemical environment. Native extracellular matrices sequester numerous bioactive species, making microenvironment biochemically active. On other hand, most 3D-printed constructs have limited activity, serving merely as mechanical scaffolding. Here we demonstrate scaffolding through integration biocatalytic enzymes within bioink. Enzymes are attractive class biocompatible substrate-specific agents that can improve regeneration outcomes. However, difficulty in application remains providing at targeted site adequate amounts over extended time. In this work, a durable enzyme bioink extrusion-based bioprinting developed by covalently attaching globular horseradish peroxidase (HRP) gelatin methacrylate (Gel-MA) biopolymer scaffold. Upon introducing groups surface enzyme, it undergoes photo-crosslinking post-printing step with Gel-MA without compromising its activity. As result, HRP becomes fixed part hydrogel network achieves higher stability inside gel which results concentration catalytic activity longer time than solely entrapping protein hydrogel. We also cytocompatibility show capabilities precise applications engineering. Our approach offers promising solution enhance properties constructs, representing critical towards achieving biofabricated tissues.

Язык: Английский

Bioreactors: Applications and Innovations for a Sustainable and Healthy Future—A Critical Review DOI Creative Commons
Fernanda Palladino, Paulo Ricardo Franco Marcelino, Andersen Escobar Schlogl

и другие.

Applied Sciences, Год журнала: 2024, Номер 14(20), С. 9346 - 9346

Опубликована: Окт. 14, 2024

Biotechnological processes are essential for developing economies that aim to stand out in future markets. The use of bioreactors is one the most important unit operations biotechnological processes, and real-time monitoring ensure precise bioprocess control. This review presents different types bioreactors, sensors, applications other sectors. Bioreactors, controlled systems cultivating microorganisms cells, tools various fields, from scientific research industrial production. a variety sensors critical accurate, monitoring, early problem detection, reproducibility, cost reduction, increased efficiency. These benefits being realized numerous applications, including biofuel production, bioremediation leaching tissue engineering, drug manufacturing. Innovations bioreactor technology expanding opportunities more sustainable healthier future. By new integrating advanced exploring promising playing key role addressing global challenges sustainably advancing science technology.

Язык: Английский

Процитировано

14
3D bioprinting in bioremediation: a comprehensive review of principles, applications, and future directions DOI Creative Commons
Abraham Samuel Finny

PeerJ, Год журнала: 2024, Номер 12, С. e16897 - e16897

Опубликована: Фев. 8, 2024

Bioremediation is experiencing a paradigm shift by integrating three-dimensional (3D) bioprinting. This transformative approach augments the precision and versatility of engineering with functional capabilities material science to create environmental restoration strategies. comprehensive review elucidates foundational principles 3D bioprinting technology for bioremediation, its current applications in prospective avenues future research technological evolution, emphasizing intersection additive manufacturing, functionalized biosystems, remediation; this delineates how can tailor bioremediation apparatus maximize pollutant degradation removal. Innovations biofabrication have yielded bio-based biodegradable materials conducive microbial proliferation sequestration, thereby addressing contamination adhering sustainability precepts. The presents an in-depth analysis application bioprinted constructs enhancing efforts, exemplifying synergy between biological systems engineered solutions. Concurrently, critically addresses inherent challenges incorporating into diverse ecological settings, including assessing their impact, durability, integration large-scale projects. Future perspectives discussed encompass exploration novel biocompatible materials, automation convergence cutting-edge fields such as nanotechnology other emerging fields. article posits cornerstone next-generation practices, offering scalable, customizable, potentially greener solutions reclaiming contaminated environments. Through review, stakeholders science, engineering, are provided critical appraisal state potential drive forward efficacy management practices.

Язык: Английский

Процитировано

7
Biocatalysis in Water or in Non-Conventional Media? Adding the CO2 Production for the Debate DOI Creative Commons
Pablo Domı́nguez de Marı́a, Selin Kara, Fabrice Gallou

и другие.

Molecules, Год журнала: 2023, Номер 28(18), С. 6452 - 6452

Опубликована: Сен. 6, 2023

Biocatalysis can be applied in aqueous media and different non-aqueous solutions (non-conventional media). Water is a safe solvent, yet many synthesis-wise interesting substrates cannot dissolved solutions, thus low concentrations are often applied. Conversely, non-conventional may enable higher substrate loadings but at the cost of using (fossil-based) organic solvents. This paper determines CO2 production-expressed as kg CO2·kg product-1-of generic biotransformations water media, assessing both upstream downstream. The key to reaching diminished environmental footprint type wastewater treatment implemented. If used chemicals conventional (mild) treatment, production limited. other (pre)treatments for needed eliminate hazardous solvents, impacts expected (based on production). biocatalysis more sustainable during unit-the biocatalytic step-than systems. However, processes with need incorporate extractive solvents downstream processing. Both strategies result comparable if recycled least 1-2 times. Under these conditions, industrial biotransformation 100 g L-1 loading would produce 15-25 product-1 regardless media.

Язык: Английский

Процитировано

16
Optimizing Continuous Flow Biocatalysis with 3D‐Printing and Inline IR Monitoring DOI Creative Commons
Michael B. Spano, Arjun S. Pamidi, M Liu

и другие.

ChemCatChem, Год журнала: 2024, Номер unknown

Опубликована: Май 18, 2024

Abstract Enzymatic biocatalysis typically generates less waste, uses water, and minimizes energy consumption compared to traditional chemical methods. Efficient, cell‐free biosynthesis relies on the reuse of its valuable biocatalysts. Immobilization enzymes solid supports, such as enzyme carrier resins (ECRs), offers a reliable widely deployed approach maximize turnover in biosynthesis. We focus two major bottlenecks associated with optimizing biocatalysis. First, we apply our lab's 3D‐printed labware screen ECRs 96‐well mini‐reactors optimize immobilization conditions. Second, introduce inline infrared spectroscopy monitor bioreactor output productivity. Urease provides model system for examining conditions continuous assessment biocatalyst performance. As required high substrate concentrations improve process efficiency minimize urease was studied unusually – molar urea. The optimized reactor processed 3.24 L 4.00 M urea at an average volumetric productivity 13 g ⋅ −1 h over 18 achieved estimated number >17.4 kg per immobilized Type‐IX. This workflow can be generalized most biocatalytic processes could accelerate adoption greater sustainability.

Язык: Английский

Процитировано

3
3D Printing in Biocatalysis and Biosensing: From General Concepts to Practical Applications DOI Creative Commons

Jonathan Nyenhuis,

Christopher Heuer, Janina Bahnemann

и другие.

Chemistry - An Asian Journal, Год журнала: 2024, Номер unknown

Опубликована: Сен. 28, 2024

Abstract 3D printing has matured into a versatile technique that offers researchers many different methods and materials with varying properties. Nowadays, is deployed within myriad of applications, ranging from chemistry to biotechnology –including bioanalytics, biocatalysis or biosensing. Due its inherent design flexibility (which enables rapid prototyping) ease use, facilitates the relatively quick easy creation new devices unprecedented functions.. This review article describes how can be employed for research in fields biochemistry biotechnology, specifically biosensor applications. We survey relevant techniques, as well surface activation functionalization 3D‐printed materials. Finally, we show used fabrication reaction ware enzymatic assays research, generation biosensors using aptamers, antibodies, enzymes recognition elements.

Язык: Английский

Процитировано

3
Biocatalysis in packed-bed reactors: immobilization as an enabling technology DOI Creative Commons
Cristina Lía Fernández Regueiro, David Roura Padrosa, Francesca Paradisi

и другие.

Comptes Rendus Chimie, Год журнала: 2025, Номер 28(G1), С. 349 - 359

Опубликована: Апрель 7, 2025

Biocatalysis and flow chemistry are two complementary technologies that can be used to produce chemicals in a more efficient, sustainable, safe manner. Enzyme immobilization is key enabling their combination. Various methods, including carrier-free carrier-based techniques, offer distinct advantages terms of enzyme activity, stability, productivity. This review explores these strategies integration packed-bed reactors, highlighting industrial applications potential challenges.

Язык: Английский

Процитировано

0
Hierarchically engineered enzyme-packed bed reactor via 3D scaffold design and zwitterionic microenvironment for sustainable continuous catalysis DOI
Jicheng Dong, Xinlong Zhang, Fang Cheng

и другие.

Bioresource Technology, Год журнала: 2025, Номер unknown, С. 132587 - 132587

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Self-Sufficient and Autosensing Biocatalytic Inks for 3D-Printed Monolithic Bioreactors DOI
Daniel Andrés‐Sanz, Clara García‐Astrain, Uxue Aizarna-Lopetegui

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Май 25, 2025

Additive manufacturing, commonly known as three-dimensional (3D) printing, transforms simple in silico designs into real objects with accessibility, reproducibility, and precision. By merging the versatility of 3D printing inherent advantages enzymatic processes, this technology opens up new possibilities for optimizing enzyme immobilization continuous flow reactors. Here, we systematically investigate various formulations to develop an optimal biocatalytic ink capable encapsulating enzymes cofactors within a hydrogel matrix. The ink, composed agarose polyethylenimine (PEI), printed porous monoliths, improved retention cofactor absorption through ionic interactions, outperforming alternative formulations. further integrating gold nanorods system, reaction substrates intermediates (i.e., NAD+, isopropanol) can be detected operando surface enhanced Raman scattering (SERS) sensing, serving complementary tool fluorescence microscopy. Using optimized fabricated 3D-printed reactors diverse architectures evaluate their efficiency reduction ethyl acetoacetate. Reactors cross-shaped design exhibit stable product yields minimize leaching during operation. Hence, formulate print self-sufficient sustaining activity immobilized dehydrogenases reactions without addition exogenous cofactors.

Язык: Английский

Процитировано

0
Chitosan-polylactic acid composites: from seafood waste to advanced functional materials for 3D printing DOI Creative Commons
Erika Blanzeanu, Maria Minodora Marin, Marian Verziu

и другие.

Advanced Composites and Hybrid Materials, Год журнала: 2024, Номер 8(1)

Опубликована: Дек. 21, 2024

Abstract The development of functional and sustainable materials for additive manufacturing is a rapidly expanding area interest. In this context, composite blends chitosan—including commercial low medium molecular weight variants, as well laboratory-extracted chitosan from shrimp head shell waste—and polylactic acid (PLA) were prepared using extrusion molding. Filament characterization was conducted to explore the effects content on filament properties melt flow index, tensile testing, dynamic mechanical analysis (DMA), differential scanning calorimetry (DSC). morphology extruded filaments analyzed electron microscopy (SEM). Additionally, possibility incorporating high ratio metal into without compromising their printability structural integrity investigated. results indicated that certain compositions chitosan-PLA enable effective incorporation nickel, highlighting potential innovative catalyst supports. 3D printed in molten state, resulting specimens subsequently examined micro-CT. This approach seeks create an material food waste, offering circular solution transforming seafood waste advanced materials. successful integration waste-derived PLA not only enhances properties, but also demonstrates creating high-value products bio-waste, contributing environmental sustainability advancing field eco-friendly manufacturing. work highlights promising application various industrial sectors, emphasizing role promoting economy.

Язык: Английский

Процитировано

3
Biocatalytic Performance of β-Glucosidase Immobilized on 3D-Printed Single- and Multi-Channel Polylactic Acid Microreactors DOI Creative Commons

Andreas-Georgios Vasios,

Anastasia Skonta, Michaela Patila

и другие.

Micromachines, Год журнала: 2024, Номер 15(2), С. 288 - 288

Опубликована: Фев. 18, 2024

Microfluidic devices have attracted much attention in the current day owing to unique advantages they provide. However, their application for industrial use is limited due manufacturing limitations and high cost. Moreover, scaling-up process of microreactor has proven be difficult. Three-dimensional (3D) printing technology a promising solution above obstacles its ability fabricate complex structures quickly at relatively low Hence, combining microscale with 3D could enhance applicability microfluidic sector. In present work, 3D-printed single-channel immobilized enzyme volume capacity 30 μL was designed created one step via fused deposition modeling (FDM) technique, using polylactic acid (PLA) as material. The underwent surface modification chitosan, β-glucosidase from Thermotoga maritima covalently immobilized. biocatalyst retained almost 100% initial activity after incubation different temperatures, while it effectively reused up 10 successful reaction cycles. multi-channel parallel incorporating 36 channels developed, resulting significant increase enzymatic productivity.

Язык: Английский

Процитировано

1