Synthetic Biology for Designing Allostery and its Potential Biomedical Applications DOI Creative Commons
Tjaša Plaper,

Urška Knez Štibler,

Roman Jerala

и другие.

Journal of Molecular Biology, Год журнала: 2025, Номер unknown, С. 169225 - 169225

Опубликована: Май 1, 2025

Allosteric regulation of protein function, where a perturbation at one site induces conformational shift or alters dynamics distal functional site, plays key role in numerous biological processes. The ability to introduce allostery using synthetic biology principles holds significant potential both for biomedical and biotechnological applications, advancing our understanding natural allostery. By customizing target proteins sensing specific chemical physical signals, including ligand binding environmental cues, we aim allosterically modulate the function depending on selected triggers. This approach, unlike active-site targeting, offers greater specificity selectivity can couple diverse physiological Synthetic strategies have been developed recently designed allosteric regulation, design modulators such as domain insertion, generation de novo switches, application engineered mechanisms control cellular functions. We examine artificial intelligence (AI)-based generative other important milestones, challenges opportunities this field, highlighting how these approaches could be applied development new therapeutic strategies.

Язык: Английский

Synthetic Biology for Designing Allostery and its Potential Biomedical Applications DOI Creative Commons
Tjaša Plaper,

Urška Knez Štibler,

Roman Jerala

и другие.

Journal of Molecular Biology, Год журнала: 2025, Номер unknown, С. 169225 - 169225

Опубликована: Май 1, 2025

Allosteric regulation of protein function, where a perturbation at one site induces conformational shift or alters dynamics distal functional site, plays key role in numerous biological processes. The ability to introduce allostery using synthetic biology principles holds significant potential both for biomedical and biotechnological applications, advancing our understanding natural allostery. By customizing target proteins sensing specific chemical physical signals, including ligand binding environmental cues, we aim allosterically modulate the function depending on selected triggers. This approach, unlike active-site targeting, offers greater specificity selectivity can couple diverse physiological Synthetic strategies have been developed recently designed allosteric regulation, design modulators such as domain insertion, generation de novo switches, application engineered mechanisms control cellular functions. We examine artificial intelligence (AI)-based generative other important milestones, challenges opportunities this field, highlighting how these approaches could be applied development new therapeutic strategies.

Язык: Английский

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