Conjugated Linoleic Acid (CLA) Mitigates High-Fat Diet (HFD)-Induced Mammary Gland Development Impairment of Pubertal Mice via Regulating CD36 Palmitoylation and Downstream JNK–ERK Pathway

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 3, 2025

Conjugated linoleic acid (CLA) is known for antiobesity. However, the role of CLA in regulating high-fat diet (HFD)-impaired pubertal mammary gland development remains undefined. Here, female mice and HC11 cells were treated with HFD or palmitic (PA), supplemented without CLA, respectively. We found that prevented impaired exposed to HFD. In vitro, c9, t11-CLA, but not t10, c12-CLA, promoted PA-suppressed proliferation, accompanied by hindered CD36 palmitoylation localization on plasma membrane. Moreover, t11-CLA reduced formation CD36/Fyn/Lyn complex inhibited JNK pathway while activated ERK PA-treated HC11. mechanism, activation inhibition abolished t11-CLA-stimulated proliferation vivo verification, total cell membrane palmitoylation, suppressed CD36/FYN/LYN complex, HFD-fed mice. conclusion, mitigated HFD-impaired via downstream JNK–ERK pathway. These data suggested potential application ameliorating obesity-impaired development.

Язык: Английский

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Protocatechuic Acid Reduces Liver Fatty Acid Uptake in HFD‐Fed Mice Associated With the Inhibition of DHHC5‐Mediated CD36 Palmitoylation

Molecular Nutrition & Food Research, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

ABSTRACT Metabolic dysfunction‐associated steatotic liver disease (MASLD) is highly prevalent and has emerged as a pressing issue for human health. A palmitoylated cluster of differentiation 36 (CD36) promotes free fatty acid (FFA) uptake, which contributes to the development MASLD. Protocatechuic (PCA), main metabolite anthocyanins, was reported inhibit MASLD by regulating expression CD36. However, impact PCA on CD36 palmitoylation not been extensively studied. In present study, we found that could significantly reduce lipid uptake accumulation in hepatocytes decreasing palmitoylation. Inhibitors were used prove suppressed lowering zinc finger DHHC‐type palmitoyltransferase 5 (DHHC5) palmitoylation, but an acyl protein thioesterase 1 (APT1)‐dependent manner. Further experiments showed PCA‐mediated inhibition DHHC5 acyltransferase activity closely related reduction CD36/Fyn/Lyn complex. diminished ultimately lessened hepatocytes.

Язык: Английский

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Regulation of pattern-recognition receptor signaling by palmitoylation

iScience, Год журнала: 2024, Номер 28(2), С. 111667 - 111667

Опубликована: Дек. 20, 2024

Язык: Английский

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Recapitulating the potential contribution of protein S-palmitoylation in cancer

Cancer and Metastasis Reviews, Год журнала: 2024, Номер 44(1)

Опубликована: Дек. 27, 2024

Язык: Английский

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