TrxR1 is involved in the activation of Caspase-11 by regulating the oxidative-reductive status of Trx-1 DOI Creative Commons
Dongsheng Bai, Chen Zhou,

Jiaying Du

и другие.

Redox Biology, Год журнала: 2024, Номер 75, С. 103277 - 103277

Опубликована: Июль 20, 2024

Sepsis is a common complication of infections that significantly impacts the survival critically patients. Currently, effective pharmacological treatment strategies are lacking. Auranofin, known as an inhibitor Thioredoxin reductase (TrxR), exhibits anti-inflammatory activity, but its role in sepsis not well understood. Here, we demonstrate significant inhibitory effect Auranofin on cecal ligation and puncture (CLP) mouse model. In vitro, inhibits pyroptosis triggered by Caspase-11 activation. Further investigations reveal inhibiting TrxR1 suppresses macrophage induced E. coli, while TrxR2 does exhibit this effect. TrxR1, functioning reductase, regulates oxidative-reductive status Thioredoxin-1 (Trx-1). Mechanistically, modulation Trx-1's reductive activity may be involved activation-induced pyroptosis. Additionally, maintains Trx-1 oxidized state. The form interacts with Caveolin-1 (CAV1), regulating outer membrane vesicle (OMV) internalization. summary, our study suggests OMV internalization maintaining Trx-1, thereby restricting activation alleviating sepsis.

Язык: Английский

The Mechanism of Baicalin in the Treatment of Mycoplasma Pneumoniae Pneumonia by Regulating NLRP3/Caspase-1 Signaling Pathway DOI

Dan Song,

Wenfeng Wei,

Jie Zhang

и другие.

Immunological Investigations, Год журнала: 2025, Номер unknown, С. 1 - 13

Опубликована: Янв. 9, 2025

This study investigated the mechanism of baicalin (BIA) attenuating inflammatory response and lung injury in mycoplasma pneumoniae pneumonia (MPP) mice. MPP mouse models were established then treated with BIA, azithromycin, or NLRP3 inflammasome activator. Lung wet-to-dry weight (W/D) ratio weighed. Serum levels MP-IgM, C-reactive protein (CRP) bronchoalveolar lavage fluid (BALF) detected by kits, NLRP3/Caspase-1 pathway-related Western blot, IL-1β, IL-18, IL-6 TNF-α ELISA. HE staining was performed to detect injury. mice showed elevated W/D ratio, upregulated serum MP-IgM CRP BALF protein, enhanced levels, which reversed BIA azithromycin treatment, suggesting that attenuated pulmonary The tissue NLRP3, cleaved Caspase-1,Caspase-1, GSDMD-N GSDMD raised IL-1β IL-18 changes annulled inhibited pathway activation. activation partially abrogated alleviative effect on mitigates inhibiting

Язык: Английский

Процитировано

0
Sinensetin attenuates LPS-induced acute pulmonary inflammation in mice and RAW264.7 cells by modulating NF-κB p65-mediated immune resistance and STAT3-mediated tissue resilience DOI

Zaibin Xu,

Kongyan Wang,

Hui-Yu Hu

и другие.

International Immunopharmacology, Год журнала: 2025, Номер 148, С. 114101 - 114101

Опубликована: Янв. 18, 2025

Язык: Английский

Процитировано

0
Kirenol ameliorates endotoxin-induced acute lung injury by inhibiting the ERK and JNK phosphorylation–mediated NFκB pathway in mice DOI
Frank Cheau‐Feng Lin, Shih‐Pin Chen, Sheng‐Di Lin

и другие.

Inflammopharmacology, Год журнала: 2025, Номер unknown

Опубликована: Март 4, 2025

Язык: Английский

Процитировано

0
TrxR1 is involved in the activation of Caspase-11 by regulating the oxidative-reductive status of Trx-1 DOI Creative Commons
Dongsheng Bai, Chen Zhou,

Jiaying Du

и другие.

Redox Biology, Год журнала: 2024, Номер 75, С. 103277 - 103277

Опубликована: Июль 20, 2024

Sepsis is a common complication of infections that significantly impacts the survival critically patients. Currently, effective pharmacological treatment strategies are lacking. Auranofin, known as an inhibitor Thioredoxin reductase (TrxR), exhibits anti-inflammatory activity, but its role in sepsis not well understood. Here, we demonstrate significant inhibitory effect Auranofin on cecal ligation and puncture (CLP) mouse model. In vitro, inhibits pyroptosis triggered by Caspase-11 activation. Further investigations reveal inhibiting TrxR1 suppresses macrophage induced E. coli, while TrxR2 does exhibit this effect. TrxR1, functioning reductase, regulates oxidative-reductive status Thioredoxin-1 (Trx-1). Mechanistically, modulation Trx-1's reductive activity may be involved activation-induced pyroptosis. Additionally, maintains Trx-1 oxidized state. The form interacts with Caveolin-1 (CAV1), regulating outer membrane vesicle (OMV) internalization. summary, our study suggests OMV internalization maintaining Trx-1, thereby restricting activation alleviating sepsis.

Язык: Английский

Процитировано

2