Inhibition effect of AGEs formation in vitro by the two novel peptides EDYGA and DLLCIC derived from Pelodiscus sinensis

Frontiers in Nutrition, Год журнала: 2025, Номер 12

Опубликована: Янв. 30, 2025

The antioxidant activity of natural products is closely related to their antiglycation effects. This study aimed examine the and elucidate underlying mechanisms two specific peptides, EDYGA (Glu-Asp-Tyr-Gly-Ala) DLLCIC (Asp-Leu-Leu-Cys-Ile-Val), derived from protein hydrolysates Pelodiscus sinensis . Both were efficient in bovine serum albumin (BSA)/glucose model inhibit BSA glycation, while showed higher than EDYGA. Firstly, it was found that could formation NEG AGEs. Moreover, able maintain secondary structure stabilize band positions (amide I & II). Additionally, molecular simulations indicated can spontaneously interact with central site BSA, specifically at Lys114 Glu424 residues, through hydrogen bonds an energy strength −0.7 kcal/mol. Furthermore, CCK-8 morphological experiments confirmed improved cell survival against AGEs-induced cytotoxicity, EC50 values 17.64 μM for 15.08 DLLCIC. These findings serve as a significant reference development effective agents prevention glycation-mediated diseases.

Язык: Английский

---

Molecular Mechanisms of Pyroptosis in Non-alcoholic Steatohepatitis and Feasible Diagnosis and Treatment Strategies

Pharmacological Research, Год журнала: 2025, Номер unknown, С. 107754 - 107754

Опубликована: Апрель 1, 2025

Pyroptosis is a distinct form of cell death that plays critical role in intensifying inflammatory responses. It primarily occurs via the classical pathway, non-classical caspase-3/6/7/8/9-mediated pathways, and granzyme-mediated pathways. Key effector proteins involved pyroptosis process include gasdermin family pannexin-1 protein. intricately linked to onset progression non-alcoholic steatohepatitis (NASH). During development NASH, factors such as pyroptosis, innate immunity, lipotoxicity, endoplasmic reticulum stress, gut microbiota imbalance interact interweave, collectively driving disease progression. This review analyzes molecular mechanisms its pathogenesis NASH. Furthermore, it explores potential diagnostic therapeutic strategies targeting offering new avenues for improving diagnosis treatment

Язык: Английский

---

Inappropriate Diet Exacerbates Metabolic Dysfunction-Associated Steatotic Liver Disease via Abdominal Obesity

Nutrients, Год журнала: 2024, Номер 16(23), С. 4208 - 4208

Опубликована: Дек. 5, 2024

Metabolic dysfunction-associated steatotic liver disease (MASLD) represents a refined categorization of non-alcoholic fatty (NAFLD), highlighting the intricate relationship between hepatic steatosis and metabolic dysfunction. Abdominal obesity (AO), key diagnostic criterion for dysfunction, predominantly results from inappropriate diet unhealthy dietary habits. To comprehensively investigate which factors contribute to MASLD through AO understand underlying biological mechanisms, we initially conducted systematic review meta-analysis articles in PubMed database past decade, summarizing that affect AO. Subsequently, targeted searches these provided narrative mechanisms how lead exacerbates MASLD. A characterized by excessive intake energy, carbohydrates, fructose, or ultra-processed foods (UPFs) is considered inappropriate. Inappropriate leads formation enhancing pathways such as de novo lipid synthesis (DNL) liver, insulin resistance (IR), gut-liver inflammation. Dietary interventions diets can effectively intervene improve The mechanism on abdominal fat deposition energy activation enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD-1) increase endocortisol secretion. Then, accumulation visceral facilitates rapid augmented flux free acids (FFAs) initiates series deleterious effects, including oxidative stress (OS), endoplasmic reticulum (ERS), protein kinase C (PKC) pathways, Additionally, FFAs may mediate hepatocellular damage action hormones. These exacerbate progression steatohepatitis (MASH) fibrosis. Furthermore, investigating other potential influence could offer new recommendations treatment guidelines

Язык: Английский

---

DAILY YERBA MATE (Ilex paraguariensis A.St.-Hil.) INTAKE DOES NOT AFFECT GASTRIC MUCOSA OF RATS

Food and Humanity, Год журнала: 2024, Номер unknown, С. 100440 - 100440

Опубликована: Окт. 1, 2024

Язык: Английский

---