Effects of fluid shear stress duration on the mechanical properties of HeLa cells using atomic force microscopy DOI Creative Commons
Xinyao Zhao,

Xiaolong Zhang,

Fei Lei

и другие.

PLoS ONE, Год журнала: 2025, Номер 20(5), С. e0321296 - e0321296

Опубликована: Май 5, 2025

Cellular mechanical properties play a critical role in physiological and pathological processes, with fluid shear stress being key determinant. Despite its importance, the impact of on characteristics HeLa cells mechanism tumor metastasis remain poorly understood. This study aims to investigate effects varying durations cells, thereby elucidating interactions between flow environment cancer during metastasis. We established an vitro cell experimental system analyzed field within parallel plate chamber using computational dynamics software. Atomic force microscopy was used measure at different time points under 10 dyn/cm², value representative conditions. analysis confirmed stability laminar uniformity chamber. The results revealed that increasing exposure duration, exhibited fusiform shape, reduction height significant decrease Young’s modulus. By integrating atomic system, this demonstrates substantial influence cells. provides novel insights into behavior vivo environment. Our findings enhance understanding cellular property regulation offer valuable for biomedicine engineering research.

Язык: Английский

Sensing Surface Tension for Evaluating Circulating Tumor Cell Transiting Behaviors at Narrow Vessels DOI
Lei Fan, Dong Ma,

Zhixin Wu

и другие.

Sensors and Actuators A Physical, Год журнала: 2025, Номер unknown, С. 116599 - 116599

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Effects of fluid shear stress duration on the mechanical properties of HeLa cells using atomic force microscopy DOI Creative Commons
Xinyao Zhao,

Xiaolong Zhang,

Fei Lei

и другие.

PLoS ONE, Год журнала: 2025, Номер 20(5), С. e0321296 - e0321296

Опубликована: Май 5, 2025

Cellular mechanical properties play a critical role in physiological and pathological processes, with fluid shear stress being key determinant. Despite its importance, the impact of on characteristics HeLa cells mechanism tumor metastasis remain poorly understood. This study aims to investigate effects varying durations cells, thereby elucidating interactions between flow environment cancer during metastasis. We established an vitro cell experimental system analyzed field within parallel plate chamber using computational dynamics software. Atomic force microscopy was used measure at different time points under 10 dyn/cm², value representative conditions. analysis confirmed stability laminar uniformity chamber. The results revealed that increasing exposure duration, exhibited fusiform shape, reduction height significant decrease Young’s modulus. By integrating atomic system, this demonstrates substantial influence cells. provides novel insights into behavior vivo environment. Our findings enhance understanding cellular property regulation offer valuable for biomedicine engineering research.

Язык: Английский

Процитировано

0