Microfluidic impedance flow cytometer leveraging virtual constriction microchannel and its application in leukocyte differential DOI Creative Commons

Minruihong Wang,

Jie Zhang, Xiao Chen

и другие.

Microsystems & Nanoengineering, Год журнала: 2024, Номер 10(1)

Опубликована: Дек. 16, 2024

Abstract Microfluidic impedance flow cytometry has been widely used in leukocyte differential and counting, but it faces a bottleneck due to the trade-off between detection throughput sensitivity. In this study, microfluidic cytometer based on virtual constriction microchannel was reported, which constructed by crossflow of conductive sample insulated sheath fluids with underneath micro-electrodes for measurements. Compared conventional mechanical microchannels, counterpart could effectively avoid direct physical contact cells walls maintain high throughputs, significantly reduce volume region sensitivity improvements. Using developed cytometer, pulses three leukemia cell lines, K562, Jurkat, HL-60, were detected, achieving 99.8% differentiation accuracy through use recurrent neural network. Furthermore, four white blood subpopulations (neutrophils, eosinophils, monocytes, lymphocytes) from donors classification ≥99.2%. A network model established purified applied two mixtures, resulting proportional distributions within theoretical ranges. These results indicated that achieve both sensitivity, showing great potentials clinical diagnostics analysis.

Язык: Английский

Particle manipulation under X-force fields DOI
Chun‐Dong Xue,

Yifan Yin,

Xiaoyu Xu

и другие.

Lab on a Chip, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

This review highlights recent technological advances for progress in particle manipulation under X-force fields, and forecasts the trajectory of future developments.

Язык: Английский

Процитировано

2

Progressive Approaches in Oncological Diagnosis and Surveillance: Real‐Time Impedance‐Based Techniques and Advanced Algorithms DOI
Viswambari Devi Ramaswamy, Michael Keidar

Bioelectromagnetics, Год журнала: 2025, Номер 46(1)

Опубликована: Янв. 1, 2025

ABSTRACT Cancer remains a formidable global health challenge, necessitating the development of innovative diagnostic techniques capable early detection and differentiation tumor/cancerous cells from their healthy counterparts. This review focuses on confluence advanced computational algorithms with noninvasive, label‐free impedance‐based biophysical methodologies—techniques that assess biological processes directly without need for external markers or dyes. elucidates diverse array state‐of‐the‐art technologies, illuminating distinct electrical signatures inherent to cancer vs tissues. Additionally, study probes transformative potential these modalities in recalibrating personalized treatment paradigms. These offer real‐time insights into tumor dynamics, paving way precision‐guided therapeutic interventions. By emphasizing quest continuous vivo monitoring, herald pivotal advancement overarching endeavor combat globally.

Язык: Английский

Процитировано

1

Tutorial on impedance and dielectric spectroscopy for single-cell characterisation on microfluidic platforms: theory, practice, and recent advances DOI Creative Commons
Fatemeh Dadkhah Tehrani, Michael O’Toole, David J. Collins

и другие.

Lab on a Chip, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Integration of low-frequency electrical impedance and broadband electromagnetic sensing with microfluidic devices enables high-throughput analysis cell size, membrane properties, intracellular characteristics.

Язык: Английский

Процитировано

1

Microfluidic impedance flow cytometer leveraging virtual constriction microchannel and its application in leukocyte differential DOI Creative Commons

Minruihong Wang,

Jie Zhang, Xiao Chen

и другие.

Microsystems & Nanoengineering, Год журнала: 2024, Номер 10(1)

Опубликована: Дек. 16, 2024

Abstract Microfluidic impedance flow cytometry has been widely used in leukocyte differential and counting, but it faces a bottleneck due to the trade-off between detection throughput sensitivity. In this study, microfluidic cytometer based on virtual constriction microchannel was reported, which constructed by crossflow of conductive sample insulated sheath fluids with underneath micro-electrodes for measurements. Compared conventional mechanical microchannels, counterpart could effectively avoid direct physical contact cells walls maintain high throughputs, significantly reduce volume region sensitivity improvements. Using developed cytometer, pulses three leukemia cell lines, K562, Jurkat, HL-60, were detected, achieving 99.8% differentiation accuracy through use recurrent neural network. Furthermore, four white blood subpopulations (neutrophils, eosinophils, monocytes, lymphocytes) from donors classification ≥99.2%. A network model established purified applied two mixtures, resulting proportional distributions within theoretical ranges. These results indicated that achieve both sensitivity, showing great potentials clinical diagnostics analysis.

Язык: Английский

Процитировано

3