Specific gFET‐Based Aptasensors for Monitoring of Microbiome Quality: Quantification of the Enteric Health‐Relevant Bacterium Roseburia Intestinalis DOI Creative Commons

Yiting Zhang,

Xing Hu,

Runliu Li

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер 14(4)

Опубликована: Дек. 11, 2024

Roseburia intestinalis, enriched in the gut, is closely associated with obesity, intestinal inflammation, and other diseases. A novel detection method for R. intestinalis to replace commonly used 16S rRNA sequencing technique aim developed, thus enabling real-time low-cost monitoring of gut microbiota. The optimal solution utilize rGO-FET (reduced graphene oxide field-effect transistor) functionalized aptamers. Due high sensitivity sensors electronic changes system, it anticipated achieve that traditional fluorescence techniques cannot attain. previous work reported a nucleic acid aptamer library, Ri 7_2, capable quantitatively tracking complex systems. However, due complexity library itself, large-scale industrial synthesis challenging, significantly limiting its further commercial application potential. Therefore, this study, through Next-Generation Sequencing analysis, four representative single aptamers from strategically selected, named A-Rose 1, 2, 3, 4, confirmed their excellent performance similar 7_2. Furthermore, aptamer-modified demonstrated universality detecting series biochemical analyses, providing powerful diagnostic tool clinical diagnosis intestinalis.

Язык: Английский

Aminophenol Molecular Capture Layer for Specific Molecular Sensing with Field-Effect Devices DOI

Pooja Verma,

Yuval Ben‐Shahar, Soumadri Samanta

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

There is an urgent need today for interface management with recognition layers composed of short receptor molecules, excellent specificity and affinity toward a target molecule, wide range sensing applications. The current work demonstrates specific detection G-type nerve agent, which based on nucleophilic substitution reaction between the surface-bound 4-amino-2-((dimethylamino)methyl)phenol (amino-2-DMAMP) receptors diethyl chlorophosphate (DCP) simulant. 2-DMAMP DCP are demonstrated 31P-nuclear magnetic resonance (NMR) electrospray ionization mass spectrometry (ESI-MS/MS). proposed layer utilized through design realization electronic chemosensor using meta-nanochannel field-effect transistor (MNC FET). SiO2 area MNC FET functionalized amino-2-DMAMP amine-based chemistry, response quantified. An demonstrated, coupled limit 1 pg/mL, dynamic 8 orders magnitude, linearity sensitivity. high grade pave way to specific, label-free, quantitative, low-cost, easy-to-operate, field-deployable sensors.

Язык: Английский

Процитировано

0
A Beginners Guide to SELEX and DNA Aptamers DOI
Cameron M. Stephens, Nina M. Goodey,

Ueli Gubler

и другие.

Analytical Biochemistry, Год журнала: 2025, Номер unknown, С. 115890 - 115890

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Specific gFET‐Based Aptasensors for Monitoring of Microbiome Quality: Quantification of the Enteric Health‐Relevant Bacterium Roseburia Intestinalis DOI Creative Commons

Yiting Zhang,

Xing Hu,

Runliu Li

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер 14(4)

Опубликована: Дек. 11, 2024

Roseburia intestinalis, enriched in the gut, is closely associated with obesity, intestinal inflammation, and other diseases. A novel detection method for R. intestinalis to replace commonly used 16S rRNA sequencing technique aim developed, thus enabling real-time low-cost monitoring of gut microbiota. The optimal solution utilize rGO-FET (reduced graphene oxide field-effect transistor) functionalized aptamers. Due high sensitivity sensors electronic changes system, it anticipated achieve that traditional fluorescence techniques cannot attain. previous work reported a nucleic acid aptamer library, Ri 7_2, capable quantitatively tracking complex systems. However, due complexity library itself, large-scale industrial synthesis challenging, significantly limiting its further commercial application potential. Therefore, this study, through Next-Generation Sequencing analysis, four representative single aptamers from strategically selected, named A-Rose 1, 2, 3, 4, confirmed their excellent performance similar 7_2. Furthermore, aptamer-modified demonstrated universality detecting series biochemical analyses, providing powerful diagnostic tool clinical diagnosis intestinalis.

Язык: Английский

Процитировано

2