Apoptosis induction and tumor growth suppression by hydroxyapatite nanoparticles–cisplatin combined treatment in Ehrlich solid carcinoma-bearing mice
Beni-Suef University Journal of Basic and Applied Sciences,
Год журнала:
2025,
Номер
14(1)
Опубликована: Янв. 20, 2025
Abstract
Background
Hydroxyapatite
(HAP)
resembles
the
components
of
biological
hard
tissue.
Recent
research
has
been
interested
in
biomedical
application
HAP
nanoparticles
(HAP-NPs)
cancer
treatment,
HAP-NPs
have
high
cytotoxic
activity
against
cancerous
cells,
addition,
they
are
nontoxic
to
healthy
normal
biocompatible,
biodegradable,
and
a
absorption
rate
within
Therefore,
this
study
evaluated
HAP-NPs'
antitumoral
Ehrlich
solid
carcinoma
(ESC)-bearing
mice,
we
examined
anticancer
efficacy
combined
treatment
common
chemotherapeutic
drug
such
as
Cisplatin
(CDDP)
ESC-bearing
mice.
Methods
Forty
female
mice
were
inoculated
with
200
µl
diluted
ascites
fluid
containing
approximately
two
million
viable
cells
mice's
left
thigh,
after
14
days
inoculation,
distributed
into
four
groups:
10
each.
ESC
group
was
administrated
distilled
water,
treated
orally
100
mg/kg
hydroxyapatite
nanoparticles,
CDDP
intraperitoneally
5
Cisplatin,
+
both
doses
cisplatin,
animal
conducted
for
20
days.
Antitumor
assessed
durations
DNA
damage
assessment
performed
using
comet
assay
ESC,
measured
expression
following
genes
(
P53,
Bcl2,
Bax
,)
quantitative
real-time
PCR,
apoptotic-related
proteins
(P53
Ki-67)
immunohistochemical
analysis.
A
histopathological
examination
performed.
Results
The
obtained
data
illustrated
promising
HAP-NPs,
higher
efficacy.
CDDP,
resulted
significant
P
<
0.05)
nucleic
acid
destruction,
overexpression
,
Bcl2
)
(Ki-67
P53),
causing
tumor
bulk
be
greatly
reduced
(1100,
570,
450
mm
3
),
respectively,
compared
2240
.
Conclusion
can
provoke
regulate
apoptosis,
selectively
eliminating
cells.
co-administration
synergistic
enhancement
cisplatin
Язык: Английский
Hybrid Hydroxyapatite–Metal Complex Materials Derived from Amino Acids and Nucleobases
Molecules,
Год журнала:
2024,
Номер
29(18), С. 4479 - 4479
Опубликована: Сен. 20, 2024
Calcium
phosphates
(CaPs)
and
their
substituted
derivatives
encompass
a
large
number
of
compounds
with
vast
presence
in
nature
that
have
aroused
great
interest
for
decades.
In
particular,
hydroxyapatite
(HAp,
Ca10(OH)2(PO4)6)
is
the
most
abundant
CaP
mineral
significant
biological
world,
at
least
part
due
to
being
major
compound
bones
teeth.
HAp
exhibits
excellent
properties,
such
as
safety,
stability,
hardness,
biocompatibility,
osteoconductivity,
among
others.
Even
some
its
drawbacks,
fragility,
can
be
redirected
thanks
another
essential
feature:
versatility.
This
based
on
compound’s
tendency
undergo
substitutions
constituent
ions
incorporate
or
anchor
new
molecules
surface
pores.
Thus,
affinity
biomolecules
makes
it
an
optimal
multiple
applications,
mainly,
but
not
only,
biomedical
fields.
The
present
review
provides
chemical
structural
context
explain
proteins
nucleic
acids
generate
hybrid
materials.
A
size-dependent
criterium
increasing
complexity
applied,
ranging
from
amino
acids/nucleobases
corresponding
macromolecules.
incorporation
metal
complexes
into
these
functionalized
also
discussed.
Язык: Английский