Preparation of Carbon Dots with Peroxidase-like Activity and Their Application in Staphylococcus aureus Detection and Antimicrobial Susceptibility Test DOI
Dan Zhao, Rong Wang, Changpeng Zhang

и другие.

Langmuir, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Carbon dot (CD) nanozymes with excellent biocompatibility, optical properties, and catalytic activity show great promise for microbial detection drug sensitivity testing. This study reports the synthesis of metal-doped green-emitting CDs good peroxidase-like activity, which were synthesized via a one-step hydrothermal route using thiourea, N-[3-(trimethoxysilyl)propyl]ethylenediamine, catechol as starting materials FeCl3 doping agent. In presence H2O2, catalyze oxidation 3,3',5,5'-tetramethylbenzidine (TMB), producing blue product; however, in bacteria bacterial catalase enzyme decomposes H2O2 inhibits CDs, preventing color change. The neutralizes prevents from color-changing reaction TMB. Based on CDs-TMB-H2O2 cascade system bioenzymes nanozymes, we developed rapid, sensitive, direct colorimetric method Staphylococcus aureus (S. aureus) limit 2 × 103 CFU/mL linear range 103-2 106 CFU/mL. visual was successfully applied to S. food samples. Antibiotics have different effects proliferation sensitive resistant strains, leading levels hydrolysis solution resulting varying intensities color; therefore, simple antibiotic susceptibility test. applications CD provide powerful tool detecting pathogenic food, clinical, environmental samples infections.

Язык: Английский

Preparation of Carbon Dots with Peroxidase-like Activity and Their Application in Staphylococcus aureus Detection and Antimicrobial Susceptibility Test DOI
Dan Zhao, Rong Wang, Changpeng Zhang

и другие.

Langmuir, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Carbon dot (CD) nanozymes with excellent biocompatibility, optical properties, and catalytic activity show great promise for microbial detection drug sensitivity testing. This study reports the synthesis of metal-doped green-emitting CDs good peroxidase-like activity, which were synthesized via a one-step hydrothermal route using thiourea, N-[3-(trimethoxysilyl)propyl]ethylenediamine, catechol as starting materials FeCl3 doping agent. In presence H2O2, catalyze oxidation 3,3',5,5'-tetramethylbenzidine (TMB), producing blue product; however, in bacteria bacterial catalase enzyme decomposes H2O2 inhibits CDs, preventing color change. The neutralizes prevents from color-changing reaction TMB. Based on CDs-TMB-H2O2 cascade system bioenzymes nanozymes, we developed rapid, sensitive, direct colorimetric method Staphylococcus aureus (S. aureus) limit 2 × 103 CFU/mL linear range 103-2 106 CFU/mL. visual was successfully applied to S. food samples. Antibiotics have different effects proliferation sensitive resistant strains, leading levels hydrolysis solution resulting varying intensities color; therefore, simple antibiotic susceptibility test. applications CD provide powerful tool detecting pathogenic food, clinical, environmental samples infections.

Язык: Английский

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