Dynamic Tracking of Biological Processes Using Near-Infrared Fluorescent Single-Walled Carbon Nanotubes

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер unknown

Опубликована: Окт. 8, 2024

Biological processes are characterized by dynamic and elaborate temporal patterns driven the interplay of genes, proteins, cellular components that crucial for adaptation to changing environments. This complexity spans from molecular organismal scales, necessitating their real-time monitoring tracking unravel active fuel living systems enable early disease detection, personalized medicine, drug development. Single-walled carbon nanotubes (SWCNTs), with unique physicochemical optical properties, have emerged as promising tools such processes. perspective highlights key properties SWCNTs make them ideal monitoring. Subsequently, it surveys studies utilizing track biological phenomena across hierarchical levels─from molecules cells, tissues, organs, whole organisms─acknowledging pivotal role in advancing this field. Finally, review outlines challenges future directions, aiming expand frontier using SWCNTs, contributing deeper insights novel applications biomedicine.

Язык: Английский

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Single-Walled Carbon Nanotube Probes for Protease Characterization Directly in Cell-Free Expression Reactions

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 14, 2025

Proteins can be rapidly prototyped with cell-free expression (CFE) but in most cases there is a lack of probes or assays to measure their function directly the cell lysate, thereby limiting throughput these screens. Increased needed build standardized, sequence data sets feed machine learning guided protein optimization. Herein, we describe use fluorescent single-walled carbon nanotubes (SWCNT) as effective for measuring protease activity lysate. Substrate proteins were conjugated carboxymethyl cellulose-wrapped SWCNT, yielding stable and sensitive detection limit 6.4 ng/mL bacterial from Streptomyces griseus. These successfully measured subtilisin unpurified CFE reactions, surpassing commercial assays. Furthermore, they enabled continuous monitoring during synthesis both purified lysate-based systems without compromising expression. Surface passivation techniques, such pre-incubation lysate supplement components, reduced initial signal loss improved probe stability complex environment. modular used, described, high-throughput screening optimization proteases and, change substrate, wider range other hydrolases.

Язык: Английский

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Controlled Quantum Well Formation on DNA-Wrapped Carbon Nanotubes via Peroxide-Mediated Aryl Diazonium Reduction

Nano Letters, Год журнала: 2025, Номер unknown

Опубликована: Фев. 3, 2025

Quantum well defect-modified single-walled carbon nanotubes are nanomaterials with wide-ranging applications in biosensing, imaging, quantum computing, and catalysis. The most common method for covalent functionalization of biosensing involves reactions aryl diazonium salts to generate sp3 defect sites, commonly followed by wrapping single-stranded DNA aqueous dispersion. We describe herein a rapid reaction directly compatible DNA-wrapped nanotubes, mediated hydrogen peroxide. uses mild conditions at physiological pH can be easily monitored real-time via fluorescence analysis control the degree functionalization. Overall, this greatly simplifies production covalently functionalized expanding their potential industrial biomedical applications.

Язык: Английский

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Single-walled carbon nanotubes sensors: Preparation and bio-application advances

Pharmaceutical Science Advances, Год журнала: 2025, Номер unknown, С. 100064 - 100064

Опубликована: Фев. 1, 2025

Язык: Английский

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Effect of Polyelectrolytes on the Photoluminescence of Single-Walled Carbon Nanotubes─Experimental and Simulation Studies

ACS Omega, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Hybrid nanoscale materials exhibit unique properties that are suitable for advanced technologies. To achieve a tunable material–property relationship, it is crucial to understand how these molecules assemble form hybrid structures and the impact on an individual material's characteristics from such molecular assemblies. A material comprises optically active semiconducting single-walled carbon nanotube (SWCNT) polyelectrolyte could bring new opportunities optoelectronic nanophotonic materials. Here, we used combined approach included experimental observation dynamics (MD) simulations investigate assembly of SWCNT–polyelectrolyte influence polymer nanotube's optical behavior. We report sodium poly(styrenesulfonate) (PSS), aromatic anionic polyelectrolyte, dispersed SWCNTs in water, but with exceptionally weak photoluminescence. MD showed PSS wrapped around SWCNT via π–π interactions between graphitic side walls substituents PSS. Notably, group, which directly attached ring polymer, remarkably close surface. Accumulation excessive negative charges exciton behavior affect its gain further insight, introduced polycationic into PSS–SWCNT complex neutralize charge reduce net surface density SWCNT. Results decrease overall charge, evidenced zeta potential measurements, subtle increase photoluminescence intensity upon cationic addition. Snapshots simulation decreased system. These results show polyelectrolytes can through interaction. Understanding polyelectrolyte-nanotube be essential developing carbon-nanotube-based probes, sensors, devices.

Язык: Английский

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Near‐Infrared Fluorescent Single‐Walled Carbon Nanotubes for Biosensing

Small, Год журнала: 2025, Номер unknown

Опубликована: Май 2, 2025

Abstract The objective of this article is to provide a comprehensive overview the recent advancements in biosensing using near‐infrared (NIR) fluorescent single‐walled carbon nanotubes (SWCNTs). SWCNTs are cylindrical structures formed by rolling up graphene layer, with their chiral index (n,m) defining diameter and electronic, mechanical, optical properties, making them metallic, semimetallic, or semiconducting. semiconducting variants feature NIR fluorescence, which offers significant advantages for biological imaging sensing due deep tissue penetration minimal background interference. Moreover, highly photostable, demonstrating resistance photobleaching blinking. Owing these unique have been widely used as probes monitoring broad spectrum analytes, ranging from small molecules macromolecules. This review explores photophysics SWCNTs, suitability biosensing, strategies developing effective SWCNT‐based sensors. begins photophysical highlighting relevance followed key technical concepts. Additionally, principles, methods optimizing functionalized diverse approaches also covered. Overall, intends foundational understanding equipping readers knowledge needed explore apply powerful nanomaterials applications.

Язык: Английский

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Scalable Photonic Nose Development through Corona Phase Molecular Recognition

ACS Sensors, Год журнала: 2024, Номер 9(12), С. 6311 - 6319

Опубликована: Дек. 4, 2024

Breath sensors promise early disease diagnosis through noninvasive, rapid analysis, but have struggled to reach clinical use due challenges in scalability and multivariate data extraction. The current breath sensor design necessitates various channel materials surface functionalization methods, which delays the process. Additionally, limited options for that provide optimum sensitivity selectivity further restrict array size a maximum of only 10 20 channels. To address these limitations, we propose sensing process based on Corona Phase Molecular Recognition (CoPhMoRe), enables creation an expansive library nanoparticle interfaces broad fingerprints multiple analytes breath. Although CoPhMoRe has predominantly been utilized liquid-phase sensing, its recent application gas-phase shown significant potential sensing. We introduce demonstrations field present concept CoPhMoRe-based photonic-nose array, leveraging fluorescent nanomaterials such as near-infrared single-walled carbon nanotubes. identified four critical milestones translating into practical applications.

Язык: Английский

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Single-Walled Carbon Nanotubes as Optical Transducers for Nanobiosensors In Vivo

ACS Nano, Год журнала: 2024, Номер 18(52), С. 35164 - 35181

Опубликована: Дек. 19, 2024

Semiconducting single-walled carbon nanotubes (SWCNTs) may serve as signal transducers for nanobiosensors. Recent studies have developed innovative methods of engineering molecularly specific sensors, while others devised deploying such sensors within live animals and plants. These advances potentiate the use implantable, noninvasive biosensors continuous drug, disease, contaminant monitoring based on optical properties (SWCNTs). Such tools substantial potential to improve disease diagnostics, prognosis, drug safety, therapeutic response, patient compliance. Outside clinical applications, also in environmental or research lab. However, work remains be done realize these goals through further materials science engineering. Here, we review current landscape quantitative SWCNT-based that been deployed living plants animals. Specifically, focused this deploy vivo well analytes detected by SWCNTs vivo. Finally, evaluated future directions take advantage promise outlined here toward field-deployable implantable patients.

Язык: Английский

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Bend Sensors Based on Nanocellulose Composites: Influence of Configuration of Resistive Strain Gauge

Radioelectronics and Communications Systems, Год журнала: 2023, Номер 66(10), С. 556 - 562

Опубликована: Окт. 1, 2023

Язык: Английский

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