Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor DOI Creative Commons
Mingyuan Sun, Zhenxiao Yu, Shuai Wang

и другие.

Nano-Micro Letters, Год журнала: 2025, Номер 17(1)

Опубликована: Апрель 30, 2025

Abstract Amplification-free, highly sensitive, and specific nucleic acid detection is crucial for health monitoring diagnosis. The type III CRISPR-Cas10 system, which provides viral immunity through CRISPR-associated protein effectors, enables a new amplification-free diagnostic tool. In this study, we develop CRISPR-graphene field-effect transistors (GFETs) biosensor by combining the system with GFETs direct detection. This exploits target RNA-activated continuous ssDNA cleavage activity of dCsm3 effector high charge density hairpin DNA reporter on GFET channel to achieve label-free, amplification-free, RNA CRISPR-GFET exhibits excellent performance in detecting medium-length RNAs miRNAs, limits at aM level broad linear range 10 −15 −11 M −9 miRNAs. It shows sensitivity throat swabs serum samples, distinguishing between healthy individuals (N = 5) breast cancer patients 6) without need extraction, purification, or amplification. platform mitigates risks associated amplification cross-contamination, making it versatile scalable tool molecular diagnostics human health.

Язык: Английский

Research Progress of CRISPR-Cas System in Medical Diagnosis DOI Open Access

霞 郑

Journal of Clinical Personalized Medicine, Год журнала: 2025, Номер 04(02), С. 231 - 239

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor DOI Creative Commons
Mingyuan Sun, Zhenxiao Yu, Shuai Wang

и другие.

Nano-Micro Letters, Год журнала: 2025, Номер 17(1)

Опубликована: Апрель 30, 2025

Abstract Amplification-free, highly sensitive, and specific nucleic acid detection is crucial for health monitoring diagnosis. The type III CRISPR-Cas10 system, which provides viral immunity through CRISPR-associated protein effectors, enables a new amplification-free diagnostic tool. In this study, we develop CRISPR-graphene field-effect transistors (GFETs) biosensor by combining the system with GFETs direct detection. This exploits target RNA-activated continuous ssDNA cleavage activity of dCsm3 effector high charge density hairpin DNA reporter on GFET channel to achieve label-free, amplification-free, RNA CRISPR-GFET exhibits excellent performance in detecting medium-length RNAs miRNAs, limits at aM level broad linear range 10 −15 −11 M −9 miRNAs. It shows sensitivity throat swabs serum samples, distinguishing between healthy individuals (N = 5) breast cancer patients 6) without need extraction, purification, or amplification. platform mitigates risks associated amplification cross-contamination, making it versatile scalable tool molecular diagnostics human health.

Язык: Английский

Процитировано

0