bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 11, 2025

Abstract Background Endozoicomonas is a widely distributed genus of marine bacteria, associated with various organisms, and recognized for its ecological importance in host health, nutrient cycling, disease dynamics. Despite significance, genomic features remain poorly characterized due to limited availability high-quality genome assemblies. Results In this study, we sequenced 5 novel strains re-sequenced 1 known strain improve resolution. By integrating these 6 genomes 31 others that were publicly available, identified distinct, coral-associated clade not by the previous two-clade classification. Pan-genomic analysis revealed significant variation genetic trait distribution among clades. Notably, lacks quorum sensing capabilities, suggesting resistance quenching mechanisms. It also ability synthesize transport vitamin B12, indicating it primary source holobionts. A remarkable feature abundance giant proteins, ranging from 15 65 kbp. We 92 such which clustered into three major groups based on amino acid similarity, each specialized functions, as antimicrobial synthesis, exotoxin production, cell adhesion. Additionally, explored prophages CRISPR-Cas systems. found acquired diverse sources via infection or other types gene transfer. sequences suggest independent evolutionary trajectories both prophage acquisition phylogenetic lineage, implying potential influence geographic environmental pressures. Conclusions This study provides new insights diversity adaptation hosts. Identification features, including deficiencies B12 synthesis sensing, presence prophages, systems, underscores roles These findings open avenues research interactions.

Язык: Английский