Toxicity and Metabolomic Dysfunction Invoked by Febrifugin, a Harmful Component of Edible Nut of Swietenia macrophylla DOI Open Access
Xiaoyue Zhang,

Qinyang Song,

Hanghang Zheng

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(17), С. 9753 - 9753

Опубликована: Сен. 9, 2024

Swietenia macrophylla fruit is a valuable and historically significant medicinal plant with anti-hypertension anti-diabetes. We identified toxic component, Febrifugin, from the edible part of nut following zebrafish toxicity-guided isolation. Febrifugin mexicanolide-type limonoid compound. The factor induced acute toxicity in zebrafish, including yolk sac edema pericardial edema, reduced body length, decreased melanin deposition, presented skeletal developmental issues. Further exploration mechanism through metabolomics revealed that caused changes 13 metabolites larvae, which are involved pentose phosphate, tricarboxylic acid (TCA) cycle, amino biosynthesis. bioassay oxidative stress capacity qRT-PCR measurement showed compound significantly affected h6pd gene phosphate pathway mRNA expression cs, idh3a, fh, shda genes TCA leading to reactive oxygen species (ROS) accumulation notable decrease glutathione (GSH) activity zebrafish. These findings provide basis for rational use S. as raise awareness safety plants.

Язык: Английский

Toxicity and Metabolomic Dysfunction Invoked by Febrifugin, a Harmful Component of Edible Nut of Swietenia macrophylla DOI Open Access
Xiaoyue Zhang,

Qinyang Song,

Hanghang Zheng

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(17), С. 9753 - 9753

Опубликована: Сен. 9, 2024

Swietenia macrophylla fruit is a valuable and historically significant medicinal plant with anti-hypertension anti-diabetes. We identified toxic component, Febrifugin, from the edible part of nut following zebrafish toxicity-guided isolation. Febrifugin mexicanolide-type limonoid compound. The factor induced acute toxicity in zebrafish, including yolk sac edema pericardial edema, reduced body length, decreased melanin deposition, presented skeletal developmental issues. Further exploration mechanism through metabolomics revealed that caused changes 13 metabolites larvae, which are involved pentose phosphate, tricarboxylic acid (TCA) cycle, amino biosynthesis. bioassay oxidative stress capacity qRT-PCR measurement showed compound significantly affected h6pd gene phosphate pathway mRNA expression cs, idh3a, fh, shda genes TCA leading to reactive oxygen species (ROS) accumulation notable decrease glutathione (GSH) activity zebrafish. These findings provide basis for rational use S. as raise awareness safety plants.

Язык: Английский

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