Engineered Cell‐Derived Nanovesicles with CAR and PH20 for Enhanced Targeted Photodynamic Cancer Therapy and Tumor Microenvironment Modulation DOI Open Access
Hongjun Oh, Gye Ryeol Park, Jieun Han

и другие.

Advanced Functional Materials, Год журнала: 2024, Номер unknown

Опубликована: Дек. 31, 2024

Abstract Photodynamic therapy (PDT) is a promising cancer treatment, but its clinical use limited by nontargeted photosensitizers (PS) that accumulate in normal tissues, causing adverse effects, and poor penetration tumor tissues due to the dense extracellular matrix (ECM). Here an innovative approach presented using cell‐derived nanovesicles (CNVs) engineered with chimeric antigen receptor (CAR) hyaluronidase PH20 enhance targeted PDT. The CAR–PH20 CNVs, loaded photosensitizer pheophorbide (PheoA), specifically target HER2‐expressing cells degrade hyaluronic acid microenvironment (TME), improving drug distribution. In vitro vivo experiments demonstrate increased reactive oxygen species (ROS) generation, improved retention, enhanced therapeutic efficacy compared conventional methods. When combined laser irradiation, these CNVs induce significant cell apoptosis inhibit growth mouse models, while minimizing toxicity tissues. This platform offers strategy for targeted, TME‐modulating PDT efficacy, reduced side marking advance nanodrug‐based therapies.

Язык: Английский

Cubosomes and hexosomes stabilized by sorbitan monooleate as biocompatible nanoplatforms against skin metastatic human melanoma DOI Creative Commons
Marco Fornasier,

Karolina Krautforst,

Julita Kulbacka

и другие.

Journal of Colloid and Interface Science, Год журнала: 2024, Номер 677, С. 842 - 852

Опубликована: Авг. 16, 2024

Nanoparticles have become versatile assets in the medical field, providing notable benefits across diverse arenas including controlled drug delivery, imaging, and immunological assays. Among these, non-lamellar lipid nanoparticles, notably cubosomes hexosomes, showcase remarkable biocompatibility stability, rendering them as optimal choices for theranostic applications. Particularly, incorporating edge activators like sodium taurocholate enhances potential of these nanoparticles dermal transdermal overcoming stratum corneum, a first line defense our skin. This study reports on formulation monoolein-based hexosomes stabilized by Span 80 co-encapsulating Chlorin e6 coenzyme QH photodynamic therapy skin metastatic melanoma. The formulations were optimized using small-angle X-ray scattering, cryo-transmission electron microscopy confirmed presence or depending ratio between 80. Furthermore, co-loaded exhibited high encapsulation efficiencies both Ce6 QH. In vitro studies human melanoma cells (Me45) demonstrated activity loaded formulations. These findings show possibility formulating more biocompatible cancer treatment.

Язык: Английский

Процитировано

7

Liposomes and Niosomes: New trends and applications in the delivery of bioactive agents for cancer therapy DOI
Helena de Almeida Cerqueira Kodel,

Paria Alizadeh,

Samad Nejad Ebrahimi

и другие.

International Journal of Pharmaceutics, Год журнала: 2024, Номер unknown, С. 124994 - 124994

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

4

Ethosomes as a new frontier and revolutionary approach for targeted skin cancer therapy DOI
Muhammad Ahsan Waqar,

Naeem Mubarak,

Asad Majeed Khan

и другие.

International Journal of Polymeric Materials, Год журнала: 2025, Номер unknown, С. 1 - 22

Опубликована: Май 6, 2025

Язык: Английский

Процитировано

0

Mesoporous Silica Nanoparticles in Skin Cancer Therapy: Multifaceted Approaches in Drug Delivery DOI

Khaled Ahmed,

Usama Ahmad,

Juber Akhtar

и другие.

Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер unknown, С. 107021 - 107021

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0

Synthesis, Molecular Docking, and Biological Activity of New EGFR-Targeted Photosensitizers Based on Cationic Porphyrins Encapsulated into Pluronic F127 Micelles DOI

Yulia S. Bortnevskaya,

Nikita S. Zakharov,

Vadim S. Senkov

и другие.

Molecular Pharmaceutics, Год журнала: 2025, Номер unknown

Опубликована: Май 8, 2025

The development of new effective photosensitizers (PS) for photodynamic therapy (PDT) is one the important tasks in medical and organic chemistry. PSs inhibiting epidermal growth factor receptors (EGFR) overexpressed cancer cells are particular importance. In this work, we proposed design molecular docking novel hybrid based on meso-aryl-substituted porphyrins Erlotinib molecule, a clinically approved tyrosine kinase inhibitor. spacer length between macrocycles Erlotinib, hydrophilicity, hydrophobicity porphyrin ring substituents were varied obtained compounds to evaluate structure-activity relationships (SAR). Photophysical photochemical characteristics studied all received presence solubilizers suitable creation dosage forms. Nanomicelles Pluronic F127 characterized compounds. vitro biological tests three cell lines, MCF-7 (breast carcinoma), A431 (epidermoid MDA-MB-231 adenocarcinoma), normal NKE (human kidney epithelial cells) performed, which showed low dark toxicity as well light-induced activity conjugates nanomolar range. Confocal microscopy experiments preferred accumulation UB-2 lower UB-3 PSs. case UB-3, observed pronounced colocalization with early endosome antigen (EEA1). Also, apoptosis inhibition phosphorylation EGFR demonstrated compound. Thus, targeting PS containing cationic pyridyl moieties linker macrocycle can contribute antitumor PDT.

Язык: Английский

Процитировано

0

Enhanced fluorescence emission or singlet oxygen production of cationic porphyrazines and porphyrins through combination with carbon dots DOI Creative Commons
Gustavo Wander Streit,

Rafael Bernardino Rodrigues da Silva Taques,

Guillermo F. Barreto

и другие.

Photochemistry and Photobiology, Год журнала: 2025, Номер unknown

Опубликована: Май 21, 2025

Abstract A cationic porphyrin and porphyrazine with the 3‐ethylpyridyl substituent (H 2 P H Pz) their respective zinc complexes (ZnP ZnPz) were assembled to a carbon dot (CD) synthesized from citric acid ammonium citrate. titration was performed using fluorescence spectrophotometer determine stoichiometric ratio at which maximum interaction between substances occurs, as well Stern–Volmer constant intrinsic binding constant. The combination CD porphyrins or porphyrazines confirmed UV–VIS absorption spectroscopy, emission, zeta potential, Diffusion‐Ordered NMR Spectroscopy (DOSY). It observed that after combination, there is decline in of derivatives, variation emission porphyrazines, subtle increase potential compared isolated particles, translational diffusion coefficient. also found upon CD, changes photophysical properties macrocycles occur, for example, quantum yield Pz increases 0.81 ± 0.03% 1.97 0.05% while singlet oxygen ca. 70%. These results exemplify capacity boost some photosensitizers are key photodynamic therapy applications.

Язык: Английский

Процитировано

0

Santali Albi Lignum: From traditional efficacy to pharmacological properties and modern therapeutics applications DOI

Cairong Han,

Zhongrui Zhang,

Akida Adiham

и другие.

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 120031 - 120031

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0

Engineered Cell‐Derived Nanovesicles with CAR and PH20 for Enhanced Targeted Photodynamic Cancer Therapy and Tumor Microenvironment Modulation DOI Open Access
Hongjun Oh, Gye Ryeol Park, Jieun Han

и другие.

Advanced Functional Materials, Год журнала: 2024, Номер unknown

Опубликована: Дек. 31, 2024

Abstract Photodynamic therapy (PDT) is a promising cancer treatment, but its clinical use limited by nontargeted photosensitizers (PS) that accumulate in normal tissues, causing adverse effects, and poor penetration tumor tissues due to the dense extracellular matrix (ECM). Here an innovative approach presented using cell‐derived nanovesicles (CNVs) engineered with chimeric antigen receptor (CAR) hyaluronidase PH20 enhance targeted PDT. The CAR–PH20 CNVs, loaded photosensitizer pheophorbide (PheoA), specifically target HER2‐expressing cells degrade hyaluronic acid microenvironment (TME), improving drug distribution. In vitro vivo experiments demonstrate increased reactive oxygen species (ROS) generation, improved retention, enhanced therapeutic efficacy compared conventional methods. When combined laser irradiation, these CNVs induce significant cell apoptosis inhibit growth mouse models, while minimizing toxicity tissues. This platform offers strategy for targeted, TME‐modulating PDT efficacy, reduced side marking advance nanodrug‐based therapies.

Язык: Английский

Процитировано

0