Trypsin-catalyzed asymmetric aldol reactions of isatins with cyclic ketones and the mechanistic insights on activity differences at theoretical level

Journal of Biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Lipase-Catalyzed Michael Addition in ‘Water-like’ Ionic Liquids and Tertiary Amides: What Is the Role of the Enzymes?

Langmuir, Год журнала: 2025, Номер unknown

Опубликована: Май 12, 2025

Michael addition is an important reaction to form C-C bonds. Different hydrolases (e.g., lipases, proteases, and d-aminoacylase) have been reported catalyze C-C-forming reactions, but the mechanism not entirely clear. This study examined several model reactions catalyzed by lipases amino acids in various solvents found that "water-like" functionalized ionic liquids (ILs) increased yield 35-55% from 30% triglyme 17% [BMIM][Tf2N]. Interestingly, tertiary amides as remarkably (to up 65-85%) enantioselectivity (up 71-84% ee) when porcine pancreatic lipase (PPL). Our experimental, spectroscopic, computational studies discovered catalysis can be attributed basic acid residues catalysts promote addition, especially amide partially unfold protein expose its (basic) residues.

Язык: Английский

Two-step tandem synthesis of sugar-containing pyrimidine derivatives catalyzed by Lipozyme® TL IM in continuous-flow microreactors

RSC Advances, Год журнала: 2024, Номер 14(51), С. 38193 - 38199

Опубликована: Янв. 1, 2024

In continuous-flow microreactors, a two-step tandem scheme was developed to synthesize series of sugar-containing pyrimidine derivatives with excellent regioselectivity, which can effectively avoid the separation and purification intermediates.

Язык: Английский