Development and Characterization of Adeno-Associated Virus-Loaded Coaxial Electrospun Scaffolds for Potential Viral Vector Delivery DOI Open Access
Haiguang Zhang, Bing Zhou, Dong Wei

и другие.

Polymers, Год журнала: 2025, Номер 17(10), С. 1381 - 1381

Опубликована: Май 17, 2025

Gene therapy, which treats genetic diseases by fixing defective genes, has gained significant attention. Viral vectors show great potential for gene delivery but face limitations like poor targeting, uncontrolled release, and risks from high-dose can lower efficiency trigger immune responses. Loading viral onto tissue engineered scaffolds presents a promising strategy to address these challenges, their widespread application remains limited due concerns regarding vector bioactivity, scaffold biocompatibility, the stability of sustained release. An adeno-associated virus (AAV), recognized its safety, high efficiency, low immunogenicity, was employed as model virus. In this study, we developed an electrospun (AAV/PCL-PEO@Co-ES) encapsulating AAV within core–shell fibers composed polycaprolactone (PCL) polyethylene oxide (PEO) via coaxial electrospinning. This configuration ensures protection while enabling controlled The physicochemical characterization results indicated that exhibited excellent mechanical properties (tensile strength: 3.22 ± 0.48 MPa) wettability (WCA: 67.90 8.45°). vitro release cell transduction assays demonstrated AAV-loaded effectively controls transduction. Furthermore, both in vivo evaluations good biocompatibility efficient delivery. These findings highlight AAV/PCL-PEO@Co-ES safe effective platform delivery, offering valuable insights future design clinically relevant systems.

Язык: Английский

Electrospray and Assembly of PEDOT:PSS on Silver Nanowires for Multifunctional Stretchable Electronic Membranes DOI
Ming Li, Wei Peng,

Chunfa Ye

и другие.

ACS Applied Electronic Materials, Год журнала: 2025, Номер unknown

Опубликована: Фев. 10, 2025

Язык: Английский

Процитировано

0
Antibacterial antioxidant polyurethane/curcumin patch with anti-swelling ability for tissue adhesion and treatment of oral ulcers DOI
Xiaolei Guo, Lin Yang, Nan Sheng

и другие.

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 162131 - 162131

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Design of a Janus Composite Patch with Anti-Adhesive and Growth-Promoting Functions for Abdominal Wall Defect Repair DOI Creative Commons

Qingxi Hu,

Xiaoyang Hou, Hekai Shi

и другие.

Bioengineering, Год журнала: 2025, Номер 12(5), С. 522 - 522

Опубликована: Май 14, 2025

Tension-free hernioplasty has effectively reduced postoperative recurrence and mitigated complications by employing polymer patches. However, clinically used patches often fall short in terms of the anti-deformation, anti-adhesion, tissue integration functions, which can result visceral adhesions foreign body reactions after implantation. In this study, a Janus three-layer composite patch was developed for abdominal wall defect repair using combination 3D printing, electrospraying, electrospinning technologies. On side, dense electrospun polyvinyl alcohol/sodium hyaluronate (PVA/HA) scaffold fabricated to inhibit cell adhesion. The middle layer, composed polycaprolactone (PCL), provided mechanical support. muscle-facing loose porous nanofiber created through electrospraying electrospinning, promoting adhesion migration facilitate regeneration. Mechanical testing demonstrated that possessed excellent tensile strength (23.58 N/cm), surpassing clinical standard (16 N/cm). Both vitro vivo evaluations confirmed patch's outstanding biocompatibility. Compared with control PCL patch, significantly enhanced animal models. Collectively, integrated tissue-regenerative properties, providing promising solution effective repair.

Язык: Английский

Процитировано

0
Development and Characterization of Adeno-Associated Virus-Loaded Coaxial Electrospun Scaffolds for Potential Viral Vector Delivery DOI Open Access
Haiguang Zhang, Bing Zhou, Dong Wei

и другие.

Polymers, Год журнала: 2025, Номер 17(10), С. 1381 - 1381

Опубликована: Май 17, 2025

Gene therapy, which treats genetic diseases by fixing defective genes, has gained significant attention. Viral vectors show great potential for gene delivery but face limitations like poor targeting, uncontrolled release, and risks from high-dose can lower efficiency trigger immune responses. Loading viral onto tissue engineered scaffolds presents a promising strategy to address these challenges, their widespread application remains limited due concerns regarding vector bioactivity, scaffold biocompatibility, the stability of sustained release. An adeno-associated virus (AAV), recognized its safety, high efficiency, low immunogenicity, was employed as model virus. In this study, we developed an electrospun (AAV/PCL-PEO@Co-ES) encapsulating AAV within core–shell fibers composed polycaprolactone (PCL) polyethylene oxide (PEO) via coaxial electrospinning. This configuration ensures protection while enabling controlled The physicochemical characterization results indicated that exhibited excellent mechanical properties (tensile strength: 3.22 ± 0.48 MPa) wettability (WCA: 67.90 8.45°). vitro release cell transduction assays demonstrated AAV-loaded effectively controls transduction. Furthermore, both in vivo evaluations good biocompatibility efficient delivery. These findings highlight AAV/PCL-PEO@Co-ES safe effective platform delivery, offering valuable insights future design clinically relevant systems.

Язык: Английский

Процитировано

0