Programmable Coacervate Droplets via Reaction-Coupled Liquid–Liquid Phase Separation (LLPS) and Competitive Inhibition DOI

Satyajit Patra,

Bhawna Sharma, Subi J. George

и другие.

Journal of the American Chemical Society, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

Membraneless biomolecular condensates formed by liquid–liquid phase separation (LLPS) are crucial for many spatiotemporal biological functions. Designing synthetic mimics to emulate and understand LLPS is an active area of research, which has led the development coacervate droplets through elegant bioinspired designs. However, recent interest in this field shifted toward designing programmable coacervates impart control over these liquid phases. Herein, we demonstrate programming systems employing concepts competitive binding reaction-coupled assembly involving dynamic covalent bonds. Our results utilize small building blocks that follow a simple coacervation mechanism, distinguishing approach from previously reported complex coacervates, often rely on reaction-controlled generation one components. We introduce using bonds (boronate esters) chromophoric appended with terminal boronic acid groups. Upon reaction substrates (monosaccharides), form molecular structures resembling "sticker-and-spacer" designs coacervation, leading reaction-driven, temporally controlled process. The differential reactivity various monosaccharides, combined reversibility bonds, enables binding-driven growth, inhibition, dissolution process, offering new strategies reminiscent protein-induced inhibition condensates. Detailed spectroscopic probing kinetic analyses provide mechanistic insights into autocatalytic growth processes, revealing glucose-selective nature system. Finally, coupling reactions temporal pH modulation transient can be visualized confocal microscopy. anticipate will pave way novel biorelevant emergent properties.

Язык: Английский

Programmable Coacervate Droplets via Reaction-Coupled Liquid–Liquid Phase Separation (LLPS) and Competitive Inhibition DOI

Satyajit Patra,

Bhawna Sharma, Subi J. George

и другие.

Journal of the American Chemical Society, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

Membraneless biomolecular condensates formed by liquid–liquid phase separation (LLPS) are crucial for many spatiotemporal biological functions. Designing synthetic mimics to emulate and understand LLPS is an active area of research, which has led the development coacervate droplets through elegant bioinspired designs. However, recent interest in this field shifted toward designing programmable coacervates impart control over these liquid phases. Herein, we demonstrate programming systems employing concepts competitive binding reaction-coupled assembly involving dynamic covalent bonds. Our results utilize small building blocks that follow a simple coacervation mechanism, distinguishing approach from previously reported complex coacervates, often rely on reaction-controlled generation one components. We introduce using bonds (boronate esters) chromophoric appended with terminal boronic acid groups. Upon reaction substrates (monosaccharides), form molecular structures resembling "sticker-and-spacer" designs coacervation, leading reaction-driven, temporally controlled process. The differential reactivity various monosaccharides, combined reversibility bonds, enables binding-driven growth, inhibition, dissolution process, offering new strategies reminiscent protein-induced inhibition condensates. Detailed spectroscopic probing kinetic analyses provide mechanistic insights into autocatalytic growth processes, revealing glucose-selective nature system. Finally, coupling reactions temporal pH modulation transient can be visualized confocal microscopy. anticipate will pave way novel biorelevant emergent properties.

Язык: Английский

Процитировано

0