Compartmentalised mucosal and blood immunity to SARS-CoV-2 associated with high seroprevalence before Delta wave in Africa DOI Creative Commons
Ya Jankey Jagne, Dawda Jobe, Alansana Darboe

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Окт. 14, 2024

Abstract Background The reported number of SARS-CoV-2 cases and deaths are lower in Africa compared to many high-income countries. However, African cohorts, detailed characterisation mucosal T cell immunity limited. We assessed SARS-CoV-2-specific immune landscape Gambia pre-Delta variant July 2021. Methods A cross-sectional assessment 349 unvaccinated individuals from 52 Gambian households was performed between March - June spike (S) nucleocapsid (N) specific binding antibodies were measured by ELISA, variant-specific serum neutralizing-antibodies (NAb) viral pseudotype assays nasal fluid IgA mesoscale discovery assay. T-cell responses evaluated using ELISpot Results show that adjusted seroprevalence anti-Spike 56.7% (95% confidence interval (CI) 49.0–64.0), which children < 5 years (26.2%, 13.9–43.8) 5–17 (46.4%, 36.2–56.7) adults 18–49 (78.4%, 68.8–85.8). In spike-seropositive individuals, NAb titres highest Alpha (median IC50 110), with 27% showing pre-existing Delta > 1:50. Whilst significantly higher 34% spike-seronegative showed reactivity one or more antigen pools. Strong correlations within T-cell, IgA, observed. Conclusion High The-Gambia induced blood immunity, reducing Omicron impact. Children relatively protected infection. seronegative may indicate either pre-pandemic cross-reactivity a dominated response infection absent poor humoral responses.

Язык: Английский

High SARS-CoV-2 incidence and asymptomatic fraction during Delta and Omicron BA.1 waves in The Gambia DOI Creative Commons
Sheikh Jarju, Rhys Wenlock,

Madikoi Danso

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Май 7, 2024

Abstract Little is known about SARS-CoV-2 infection risk in African countries with high levels of infection-driven immunity and low vaccine coverage. We conducted a prospective cohort study 349 participants from 52 households The Gambia between March 2021 June 2022, routine weekly RT-PCR 6-monthly serology. Attack rates 45% 57% were seen during Delta Omicron BA.1 waves respectively. Eighty-four percent RT-PCR-positive infections asymptomatic. Children under 5-years had lower incidence than 18-49-year-olds. One prior reduced the wave only, ≥2 required to reduce early lineage viruses. In an population coverage, we find attack waves, proportion asymptomatic young children remaining relatively protected infection.

Язык: Английский

Процитировано

8
Compartmentalised mucosal and blood immunity to SARS-CoV-2 is associated with high seroprevalence before the Delta wave in Africa DOI Creative Commons
Ya Jankey Jagne, Dawda Jobe, Alansana Darboe

и другие.

Communications Medicine, Год журнала: 2025, Номер 5(1)

Опубликована: Май 16, 2025

The reported number of SARS-CoV-2 cases and deaths are lower in Africa compared to many high-income countries. However, African cohorts, detailed characterisation mucosal T cell immunity limited. We assessed the SARS-CoV-2-specific immune landscape Gambia during presence pre-Delta variant July 2021. A cross-sectional assessment 349 unvaccinated individuals from 52 Gambian households was performed between March-June spike (S) nucleocapsid (N) specific binding antibodies were measured by ELISA, variant-specific serum neutralizing-antibodies (NAb) viral pseudotype assays nasal fluid IgA mesoscale discovery assay. T-cell responses evaluated using ELISpot show that adjusted anti-Spike antibody seroprevalence is 56.7% (95% confidence interval (CI) 49.0-64.0), with rates children <5 years (26.2%, 13.9-43.8) 5-17 (46.4%, 36.2-56.7) adults 18-49 (78.4%, 68.8-85.8). Among spike-seropositive individuals, NAb titres highest against Alpha (median IC50 110), 27% showing pre-existing Delta >1:50. higher although 34% spike-seronegative at least one antigen pool. observe strong correlations within T-cell, IgA, responses. High The-Gambia induce blood immunity, reducing Omicron impact. Children relatively protected infection. seronegative may indicate either pre-pandemic cross-reactivity or a dominated response infection absent poor humoral

Язык: Английский

Процитировано

0
Compartmentalised mucosal and blood immunity to SARS-CoV-2 associated with high seroprevalence before Delta wave in Africa DOI Creative Commons
Ya Jankey Jagne, Dawda Jobe, Alansana Darboe

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Окт. 14, 2024

Abstract Background The reported number of SARS-CoV-2 cases and deaths are lower in Africa compared to many high-income countries. However, African cohorts, detailed characterisation mucosal T cell immunity limited. We assessed SARS-CoV-2-specific immune landscape Gambia pre-Delta variant July 2021. Methods A cross-sectional assessment 349 unvaccinated individuals from 52 Gambian households was performed between March - June spike (S) nucleocapsid (N) specific binding antibodies were measured by ELISA, variant-specific serum neutralizing-antibodies (NAb) viral pseudotype assays nasal fluid IgA mesoscale discovery assay. T-cell responses evaluated using ELISpot Results show that adjusted seroprevalence anti-Spike 56.7% (95% confidence interval (CI) 49.0–64.0), which children < 5 years (26.2%, 13.9–43.8) 5–17 (46.4%, 36.2–56.7) adults 18–49 (78.4%, 68.8–85.8). In spike-seropositive individuals, NAb titres highest Alpha (median IC50 110), with 27% showing pre-existing Delta > 1:50. Whilst significantly higher 34% spike-seronegative showed reactivity one or more antigen pools. Strong correlations within T-cell, IgA, observed. Conclusion High The-Gambia induced blood immunity, reducing Omicron impact. Children relatively protected infection. seronegative may indicate either pre-pandemic cross-reactivity a dominated response infection absent poor humoral responses.

Язык: Английский

Процитировано

0