Biochemical Genetics, Год журнала: 2023, Номер 62(4), С. 3111 - 3135
Опубликована: Дек. 9, 2023
Язык: Английский
Frontiers in Oncology, Год журнала: 2021, Номер 11
Опубликована: Окт. 15, 2021
MicroRNAs (miRNAs) are noncoding RNAs that have been identified as important posttranscriptional regulators of gene expression. miRNAs production is controlled at multiple levels, including transcriptional and regulation. Extensive profiling studies shown the regulation mature expression plays a causal role in cancer development progression. to act tumor suppressors (TS) or oncogenes based on their modulating effect target genes. Upregulation oncogenic blocks TS genes leads formation. In contrast, downregulation with function increases translation oncogenes. Several exhibiting properties studied. this review we focus recent cells microenvironment (TME). Furthermore, discuss how miRNA impacts aggressiveness cells, mechanism regulate its The study mechanisms TME may paved way understand critical progression likely clinical implications near future. Finally, potential roles specific biomarkers for diagnosis prognosis replacement suppressive using mimics could be promising approaches therapy.
Язык: Английский
Процитировано
159
Frontiers in Genetics, Год журнала: 2022, Номер 13
Опубликована: Июль 15, 2022
Background: Clear cell renal carcinoma (ccRCC) accounts for 80% of all kidney cancers and has a poor prognosis. Recent studies have shown that copper-dependent, regulated death differs from previously known mechanisms (apoptosis, ferroptosis, necroptosis) is dependent on mitochondrial respiration (Tsvetkov et al., Science, 2022, 375 (6586), 1254-1261). Studies also suggested targeting cuproptosis may be novel therapeutic strategy cancer therapy. In ccRCC, both lncRNA were critical, but the not fully understood. The aim our study was to construct prognostic profile based cuproptosis-associated lncRNAs predict prognosis ccRCC immune clear (ccRCC). Methods: We downloaded transcriptional clinical information Cancer Genome Atlas (TCGA). Co-expression network analysis, Cox regression method, least absolute shrinkage selection operator (LASSO) method used identify risk model. addition, predictive performance model validated recognized by an integrated approach. then constructed nomogram patients. Differences in biological function investigated GO, KEGG, immunoassay. Immunotherapy response measured using tumor mutational burden (TMB) dysfunction rejection (TIDE) scores. Results: panel 10 (HHLA3, H1-10-AS1, PICSAR, LINC02027, SNHG15, SNHG8, LINC00471, EIF1B-AS1, LINC02154, MINCR) prediction Kaplan-Meier ROC curves showed feature had acceptable validity TCGA training, test, complete groups. higher diagnostic efficiency compared other features. analysis Immune infiltration ssGSEA further confirmed features significantly associated with status Notably, superimposed effect patients high-risk group high TMB resulted shorter survival. TIDE scores poorer outcome checkpoint blockade these Conclusion: ten cuproptosis-related profiles help assess molecular improve treatment options, which can applied clinic.
Язык: Английский
Процитировано
87
Frontiers in Oncology, Год журнала: 2025, Номер 15
Опубликована: Апрель 3, 2025
Cancer is a complex and highly lethal disease marked by unchecked cell proliferation, aggressive behavior, strong tendency to metastasize. Despite significant advancements in cancer diagnosis treatment, challenges such as early detection difficulties, drug resistance, adverse effects of radiotherapy or chemotherapy continue threaten patient survival. MicroRNAs (miRNAs) have emerged critical regulators biology, with miR-506 being extensively studied recognized for its tumor-suppressive across multiple types. This review examines the regulatory mechanisms common cancers, focusing on role competing endogenous RNA (ceRNA) network apoptosis, migration. We also discuss potential therapeutic target overcoming resistance treatment. Overall, these insights underscore promise developing novel therapies.
Язык: Английский
Процитировано
0
Pathology - Research and Practice, Год журнала: 2023, Номер 246, С. 154480 - 154480
Опубликована: Апрель 24, 2023
Long noncoding RNAs (lncRNAs) play an important role in tumor progression. Small nucleolar RNA host gene 15 (SNHG15) is a lncRNA that has been confirmed to oncogenic multiple cancer types. However, its glycolysis and chemoresistance colorectal (CRC) unclear. The expression of SNHG15 CRC was analyzed using data from Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) databases by bioinformatics methods. Cell Counting Kit-8 (CCK-8) colony formation assays were used evaluate cell viability. sensitivity 5-fluorouracil (5-FU) detected CCK-8. Glucose absorption lactate production the impact on glycolysis. RNA-seq, real-time fluorescence quantitative reverse transcription PCR (RT-qPCR) Western blotting (WB) reveal potential molecular mechanism CRC. upregulated tissues compared with paired noncancerous tissues. Ectopic increased proliferation, 5-FU chemoresistance, cells. In contrast, knockdown inhibited Multiple pathways, including apoptosis glycolysis, potentially regulated based RNA-seq pathway enrichment analyses. RT-qPCR WB experiments promoted TYMS, BCL2, GLUT1 PKM2 conclusion, promotes regulating appears be new target for therapy.
Язык: Английский
Процитировано
10
BioMed Research International, Год журнала: 2021, Номер 2021, С. 1 - 9
Опубликована: Окт. 25, 2021
Ovarian cancer is a kind of from the female genital tract; molecular mechanism still needs to be explored. lncRNA plays vital role in tumorigenesis and development. Our aim was identify oncogenic lncRNAs ovarian explore potential mechanism. SNHG15 initially identified by using GEO datasets (GSE135886 GSE119054) validated tumor tissues cell line, identifying that upregulated cancer. Besides, high indicated poor prognosis Furthermore, knockdown suppresses proliferation promotes apoptosis. Mechanistically, through CDK6 via sponging miR-370-3p. Taken together, our findings emphasize important cancer, suggesting may promising target for
Язык: Английский
Процитировано
14
Frontiers in Oncology, Год журнала: 2023, Номер 13
Опубликована: Март 28, 2023
As oncogenes or tumor suppressor genes, lncRNAs played an important role in tumorigenesis and the progression of human cancers. The lncRNA SNHG15 has recently been revealed to be dysregulated malignant tumors, suggesting aberrant expression which contributes clinical features regulates various oncogenic processes. We have selected extensive literature focused on from electronic databases, including studies relevant its significance critical events cancer-related processes such as cell proliferation, apoptosis, autophagy, metastasis, drug resistance. This review summarized current understanding cancer, mainly focusing pathological features, known biological functions, underlying molecular mechanisms. Furthermore, well-documented effective diagnostic prognostic marker for offering novel therapeutic interventions specific subsets cancer cells.
Язык: Английский
Процитировано
5
Aging, Год журнала: 2022, Номер 14(15), С. 6358 - 6376
Опубликована: Авг. 14, 2022
Gastric cancer (GC) is one of the most aggressive malignant tumors worldwide. Ferroptosis a kind iron-dependent cell death, which proved to be closely related tumor progression. In this study, we aim at constructing ferroptosis-related lncRNAs signature predict prognosis GC and explore potential therapies.Ferroptosis-Related LncRNAs Signature for patients (FRLSG) was constructed through univariate Cox regression, LASSO algorithm, multivariate regression. Kaplan-Meier analysis, receiver operating characteristic curves, risk score plot were applied verify predictive power FRLSG. Gene Set Enrichment Analysis (GSEA) immune infiltration analyses conducted clinical value addition, drug sensitivity prediction identify chemotherapeutic drugs with therapeutic effect.Five (AC004816.1, AC005532.1, LINC01357, AL355574.1 AL049840.4) identified construct FRLSG, whose expression level in confirmed by experimental validation. curve ROC reliability effectiveness FRLSG predicting patients. Several immune-related pathways enriched high-FRLSG group, further demonstrated high status group. 19 24 candidate effect high- low-FRLSG groups, respectively.FRLSG an effective tool GC, might help prioritize therapeutics
Язык: Английский
Процитировано
8
Cancers, Год журнала: 2022, Номер 14(18), С. 4528 - 4528
Опубликована: Сен. 19, 2022
Recent advances in research have led to earlier diagnosis and targeted therapies against breast cancer, which has resulted reduced cancer-related mortality. However, the majority of deaths are due metastasis cancer cells other organs, a process that not been fully elucidated. Among factors genes implicated metastatic regulation, non-coding RNAs emerged as crucial players. This review focuses on role long intergenic noncoding (lincRNAs) small nucleolar (snoRNAs) cell metastasis. LincRNAs transcribed between two protein-coding longer than 200 nucleotides, they do code for specific protein but function regulatory molecules processes such proliferation, apoptosis, epithelial-to-mesenchymal transition, migration, invasion while most them highly elevated tissues seem competing endogenous (ceRNAs) inhibiting relevant miRNAs specifically target vital metastasis-related genes. Similarly, snoRNAs 60-300 nucleotides found nucleolus being responsible post-transcriptional modification ribosomal spliceosomal RNAs. Most hosted inside intron sequences non-protein-coding genes, also regulate affecting related cellular properties.
Язык: Английский
Процитировано
8
FEBS Journal, Год журнала: 2022, Номер 291(7), С. 1353 - 1385
Опубликована: Окт. 25, 2022
Long noncoding RNAs (lncRNAs) play crucial roles in normal physiology and are often de-regulated disease states such as cancer. Recently, a class of lncRNAs referred to the small nucleolar RNA host gene (SNHG) family have emerged important players tumourigenesis. Here, we discuss new findings describing role SNHGs gastrointestinal tumours summarize three main functions by which these promote carcinogenesis, namely: competing with endogenous RNAs, modulating protein function, regulating epigenetic marking. Furthermore, how participate different hallmarks cancer, this may serve potential biomarkers cancer diagnosis therapy.
Язык: Английский
Процитировано
8
Journal of Oncology, Год журнала: 2022, Номер 2022, С. 1 - 7
Опубликована: Июль 1, 2022
Gastric cancer is one of the most common malignancies with high mortality worldwide. It known that long noncoding RNAs (lncRNAs) play important roles in pathogenesis gastric cancer. This study investigates role lncRNA ZNF667-AS1 cells.We have applied real-time quantitative PCR (qPCR) to levels biopsies and cell lines. The effects on propagation, clonogenicity, metastasis, angiogenesis cells were evaluated by calorimetry, colony formation, migration, assays. Western blotting was used identify proteins involved invasion signaling pathways.It found downregulated biopsies. Overexpression reduced cells. Molecular mechanism studies displayed level promoted expression E-cadherin inhibited N-cadherin VEGFA, leading inhibition proliferation, cells.As a tumor suppressor gene, significantly hinders promoting inhibiting VEGFA. Therefore, could synergistic therapeutic targeting vascular endothelial cells, which represents new scheme for novel therapeutics
Язык: Английский
Процитировано
8