BIG participates in the Arg/N-degron pathways and the hypoxia response inArabidopsis thaliana DOI Creative Commons
Hongtao Zhang, Chelsea A. Rundle, Nikola Winter

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Май 27, 2023

Abstract BIG (also known as DOC1 and TIR3) is an 0.5 MDa protein that has been associated with multiple important functions in signalling development through forward genetic screens Arabidopsis thaliana . However, the biochemical function(s) of are unknown. Here, we investigated whether plays a role Arg/N-degron pathways, regulatory mechanisms which substrate fate influenced by N-terminal (Nt) residue. In Arabidopsis, PROTEOLYSIS1 (PRT1) E3 ligase specificity for aromatic amino acids, whereas PROTEOLYSIS6 (PRT6) targets basic residues. We crossed big loss-of-function allele to prt6 prt1 mutants examined stability substrates. Stability model N-degron pathway substrates was enhanced prt6-1 big-2 prt1-1 relative respective single mutants. Abundance PRT6 physiological substrates, HYPOXIA RESPONSIVE ERF (HRE)2 VERNALIZATION (VRN)2 similarly increased double mutants, without increase transcripts. Accordingly, hypoxia marker expression manner requiring arginyltransferase activity RAP-type ERFVII transcription factors. Transcriptomic analysis roots not only demonstrated synergistically plethora responsive genes mutant but also revealed other roles BIG, including regulation suberin deposition both ERFVII-dependent independent mechanisms, respectively. Our results show acts together regulate response wider processes. Significance Statement The pathways group play plant growth, development, biotic abiotic stresses. Despite rapid progress last decade, key enzymatic components remain be identified. approximately MDa, multiple, distinct plants precise this have remained enigmatic until now. Here identify new component N-recognin control

Язык: Английский

K63-linked ubiquitin chains are a global signal for endocytosis and contribute to selective autophagy in plants DOI Creative Commons
Bushra Saeed, Florian Deligne, Carla Brillada

и другие.

Current Biology, Год журнала: 2023, Номер 33(7), С. 1337 - 1345.e5

Опубликована: Март 1, 2023

Язык: Английский

Процитировано

22
BIG enhances Arg/N-degron pathway-mediated protein degradation to regulate Arabidopsis hypoxia responses and suberin deposition DOI Creative Commons
Hongtao Zhang, Chelsea A. Rundle, Nikola Winter

и другие.

The Plant Cell, Год журнала: 2024, Номер 36(9), С. 3177 - 3200

Опубликована: Апрель 12, 2024

Abstract BIG/DARK OVEREXPRESSION OF CAB1/TRANSPORT INHIBITOR RESPONSE3 is a 0.5 MDa protein associated with multiple functions in Arabidopsis (Arabidopsis thaliana) signaling and development. However, the biochemical of BIG are unknown. We investigated role for Arg/N-degron pathways, which substrate fate influenced by N-terminal residue. crossed big loss-of-function allele to 2 N-degron pathway E3 ligase mutants, proteolysis6 (prt6) prt1, examined stability substrates. Stability model substrates was enhanced prt6-1 big-2 prt1-1 relative respective single abundance PRT6 physiological substrates, HYPOXIA-RESPONSIVE ERF2 (HRE2) VERNALIZATION2 (VRN2), similarly increased prt6 double mutants. Hypoxia marker expression mutants; this constitutive response required arginyl transferase activity RAP-type Group VII ethylene factor (ERFVII) transcription factors. Transcriptomic analysis roots not only demonstrated hypoxia-responsive genes mutant prt6, but also revealed other roles BIG, including regulation suberin deposition through both ERFVII-dependent independent mechanisms, respectively. Our results show that acts together regulate hypoxia-response broader processes Arabidopsis.

Язык: Английский

Процитировано

9
Manipulation of targeted protein degradation in plant biology DOI Creative Commons
Marcela Rojas‐Pierce, Sebastian Y. Bednarek

Biochemical Society Transactions, Год журнала: 2025, Номер 53(02)

Опубликована: Апрель 9, 2025

Inducible protein degradation systems are an important but untapped resource for the study of function in plant cells. Unlike mutagenesis or transcriptional control, regulated proteins interest allows biological mechanisms highly dynamic cellular processes involving essential proteins. While targeted available research and therapeutics animals, there currently limited options biology. Targeted rely on target ubiquitination by E3 ubiquitin ligases. Systems that being developed plants can be distinguished primarily type ligase involved, including those utilize Cullin-RING ligases, bacterial novel N-end rule pathway they controlled proteolysis targeting chimeras. Target leads to proteasome vacuole, with both pathways ubiquitous endogenous control abundance plants. approaches will likely tool basic yield traits crop biotechnology.

Язык: Английский

Процитировано

0
BIG participates in the Arg/N-degron pathways and the hypoxia response inArabidopsis thaliana DOI Creative Commons
Hongtao Zhang, Chelsea A. Rundle, Nikola Winter

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Май 27, 2023

Abstract BIG (also known as DOC1 and TIR3) is an 0.5 MDa protein that has been associated with multiple important functions in signalling development through forward genetic screens Arabidopsis thaliana . However, the biochemical function(s) of are unknown. Here, we investigated whether plays a role Arg/N-degron pathways, regulatory mechanisms which substrate fate influenced by N-terminal (Nt) residue. In Arabidopsis, PROTEOLYSIS1 (PRT1) E3 ligase specificity for aromatic amino acids, whereas PROTEOLYSIS6 (PRT6) targets basic residues. We crossed big loss-of-function allele to prt6 prt1 mutants examined stability substrates. Stability model N-degron pathway substrates was enhanced prt6-1 big-2 prt1-1 relative respective single mutants. Abundance PRT6 physiological substrates, HYPOXIA RESPONSIVE ERF (HRE)2 VERNALIZATION (VRN)2 similarly increased double mutants, without increase transcripts. Accordingly, hypoxia marker expression manner requiring arginyltransferase activity RAP-type ERFVII transcription factors. Transcriptomic analysis roots not only demonstrated synergistically plethora responsive genes mutant but also revealed other roles BIG, including regulation suberin deposition both ERFVII-dependent independent mechanisms, respectively. Our results show acts together regulate response wider processes. Significance Statement The pathways group play plant growth, development, biotic abiotic stresses. Despite rapid progress last decade, key enzymatic components remain be identified. approximately MDa, multiple, distinct plants precise this have remained enigmatic until now. Here identify new component N-recognin control

Язык: Английский

Процитировано

0