Biochemistry,
Год журнала:
2021,
Номер
60(47), С. 3566 - 3581
Опубликована: Ноя. 16, 2021
In
immature
oocytes,
Balbiani
bodies
are
conserved
membraneless
condensates
implicated
in
oocyte
polarization,
the
organization
of
mitochondria,
and
long-term
organelle
RNA
storage.
Xenopus
laevis,
body
assembly
is
mediated
by
protein
Velo1.
Velo1
contains
an
N-terminal
prion-like
domain
(PLD)
that
essential
for
formation.
PLDs
have
emerged
as
a
class
intrinsically
disordered
regions
can
undergo
various
different
types
intracellular
phase
transitions
often
associated
with
dynamic,
liquid-like
condensates.
Intriguingly,
PLD
forms
solid-like
assemblies.
Here
we
sought
to
understand
why
behavior
appears
be
biophysically
distinct
from
other
PLD-containing
proteins.
Through
bioinformatic
analysis
coarse-grained
simulations,
predict
clustering
aromatic
residues
amino
acid
composition
between
aromatics
influence
condensate
material
properties,
organization,
driving
forces
assembly.
To
test
our
predictions,
redesigned
impact
targeted
sequence
changes
vivo.
We
found
design
evenly
spaced
shows
rapid
internal
dynamics,
probed
fluorescent
recovery
after
photobleaching,
even
when
recruited
into
bodies.
Our
results
suggest
might
been
selected
evolution
distinctly
clustered
maintain
structure
long-lived
oocytes.
general,
work
identifies
several
tunable
parameters
used
augment
state,
offering
road
map
synthetic
Chemical Reviews,
Год журнала:
2023,
Номер
123(14), С. 8945 - 8987
Опубликована: Март 7, 2023
Multivalent
proteins
and
nucleic
acids,
collectively
referred
to
as
multivalent
associative
biomacromolecules,
provide
the
driving
forces
for
formation
compositional
regulation
of
biomolecular
condensates.
Here,
we
review
key
concepts
phase
transitions
aqueous
solutions
specifically
that
include
folded
domains
intrinsically
disordered
regions.
The
these
systems
come
under
rubric
coupled
segregative
transitions.
underlying
processes
are
presented,
their
relevance
condensates
is
discussed.
Proceedings of the National Academy of Sciences,
Год журнала:
2023,
Номер
120(44)
Опубликована: Окт. 25, 2023
The
AlphaFold
Protein
Structure
Database
contains
predicted
structures
for
millions
of
proteins.
For
the
majority
human
proteins
that
contain
intrinsically
disordered
regions
(IDRs),
which
do
not
adopt
a
stable
structure,
it
is
generally
assumed
these
have
low
AlphaFold2
confidence
scores
reflect
low-confidence
structural
predictions.
Here,
we
show
assigns
confident
to
nearly
15%
IDRs.
By
comparison
experimental
NMR
data
subset
IDRs
are
known
conditionally
fold
(i.e.,
upon
binding
or
under
other
specific
conditions),
find
often
predicts
structure
folded
state.
Based
on
databases
fold,
estimate
can
identify
folding
at
precision
as
high
88%
10%
false
positive
rate,
remarkable
considering
IDR
were
minimally
represented
in
its
training
data.
We
disease
mutations
fivefold
enriched
over
general
and
up
80%
prokaryotes
compared
less
than
20%
eukaryotic
These
results
indicate
large
proteomes
eukaryotes
function
absence
conditional
folding,
but
acquire
folds
more
sensitive
mutations.
emphasize
predictions
reveal
functionally
relevant
plasticity
within
cannot
offer
realistic
ensemble
representations
Chemical Society Reviews,
Год журнала:
2021,
Номер
50(23), С. 13321 - 13345
Опубликована: Янв. 1, 2021
Water
prevents
adhesion
by
disrupting
the
interfacial
interactions
and
weakening
cohesive
network
of
adhesive.
This
review
summarizes
recent
developments
in
physical
chemical
design
principles
underwater
adhesives.
