Bioimplant-on-a-Chip for Facile Investigation of Periodontal Ligament Formation on Biogenic Hydroxyapatite/Ti6Al4 V Implants DOI Creative Commons
Sangbae Park,

Jae Eun Kim,

Juo Lee

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Май 13, 2025

Highly osseointegrative dental implants surrounded by reconstructed periodontal tissues represent a promising strategy for functional tooth replacement, as they mimic the structural and physiological characteristics of natural teeth. However, there is currently lack in vitro platforms that can effectively evaluate integration engineered ligament (PDL) with bioimplants. In this study, we developed bioimplant-on-a-chip (BoC) platform designed to recapitulate native PDL-cementum interface assess early stage biological performance bioimplants vitro. The BoC consists implant, calcium phosphate cement (CPC) insert, nanopatterned polydimethylsiloxane (PDMS) substrate, PDL-like tissue derived from human pulp stem cells (DPSCs). To establish viable culture conditions within platform, surface coatings cell seeding densities were optimized support formation tissue. Nanogrooved substrates incorporated guide cellular alignment, which was assessed through orientation analysis. Collagen fiber organization matrix deposition further examined indicators ligamentous maturation. Cementogenic activity evaluated immunofluorescent staining cementum protein-1 (CEMP-1) response varying biogenic hydroxyapatite (bHA) contents results demonstrated successful reproduction material-dependent differences CEMP-1 expression. This provides modular reproducible tool comparative evaluation physiologically relevant setting may be useful advancing regenerative strategies implantology.

Язык: Английский

Bioimplant-on-a-Chip for Facile Investigation of Periodontal Ligament Formation on Biogenic Hydroxyapatite/Ti6Al4 V Implants DOI Creative Commons
Sangbae Park,

Jae Eun Kim,

Juo Lee

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Май 13, 2025

Highly osseointegrative dental implants surrounded by reconstructed periodontal tissues represent a promising strategy for functional tooth replacement, as they mimic the structural and physiological characteristics of natural teeth. However, there is currently lack in vitro platforms that can effectively evaluate integration engineered ligament (PDL) with bioimplants. In this study, we developed bioimplant-on-a-chip (BoC) platform designed to recapitulate native PDL-cementum interface assess early stage biological performance bioimplants vitro. The BoC consists implant, calcium phosphate cement (CPC) insert, nanopatterned polydimethylsiloxane (PDMS) substrate, PDL-like tissue derived from human pulp stem cells (DPSCs). To establish viable culture conditions within platform, surface coatings cell seeding densities were optimized support formation tissue. Nanogrooved substrates incorporated guide cellular alignment, which was assessed through orientation analysis. Collagen fiber organization matrix deposition further examined indicators ligamentous maturation. Cementogenic activity evaluated immunofluorescent staining cementum protein-1 (CEMP-1) response varying biogenic hydroxyapatite (bHA) contents results demonstrated successful reproduction material-dependent differences CEMP-1 expression. This provides modular reproducible tool comparative evaluation physiologically relevant setting may be useful advancing regenerative strategies implantology.

Язык: Английский

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