Chitosan-Based Semen Extenders: An Approach to Antibiotic-Free Artificial Insemination in Rabbit

Antibiotics, Год журнала: 2025, Номер 14(1), С. 55 - 55

Опубликована: Янв. 9, 2025

Background/Objectives: The use of antibiotics in livestock contributes to antimicrobial resistance, highlighting the need for alternative solutions. Among these, chelating agents, like ethylenediaminetetraacetic acid (EDTA) and Chitosan, have shown potential reducing bacterial contamination seminal doses used artificial insemination (AI), while preserving sperm quality. objective this study was evaluate EDTA Chitosan as alternatives liquid storage rabbit AI doses. Methods: (20 mM) (0.05%) were tested both individually combination, by adding them semen extender, their effects compared with extenders containing or none. evaluated microbial resistance ability maintain quality vitro during refrigeration at 16 ± 1 °C 72 h. To assess efficacy, Enterococcus faecalis used. Seminal stored 24 h under commercial conditions, fertility rate total kits born evaluated. Results: Adding 0.05% extender resulted parameters load comparable those achieved refrigerated storage, yielding similar outcomes post-storage. In contrast, alone combination less effective controlling than antibiotic extenders, which also a reduction motility over period (0-72 h) negatively impacted born. Conclusions: Chitosan's protective effect on function, combined its activity, makes it promising agent

Язык: Английский

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Antimicrobial peptides and proteins as alternative antibiotics for porcine semen preservation

BMC Veterinary Research, Год журнала: 2024, Номер 20(1)

Опубликована: Июнь 12, 2024

Abstract Background Antimicrobial resistance (AMR) is nowadays a major emerging challenge for public health worldwide. The over- and misuse of antibiotics, including those cell culture, are promoting AMR while also encouraging the research employment alternative drugs. addition antibiotics to media strongly recommended in sperm preservation, being gentamicin most used boar semen. Because its continued use, several bacterial strains present semen have developed this antibiotic. peptides proteins (AMPPs) promising candidates as because their mechanism action less likely promote AMR. In study, we tested two AMPPs (lysozyme nisin; 50 500 µg/mL) possible substitutes preservation up 48 h storage. Results We found that both improved plasma membrane acrosome integrity during highest concentration lysozyme kept remaining parameters unaltered, at storage, reduced load comparable levels samples supplemented with ( p > 0.05). On other hand, nisin (500 total Enterobacteriaceae counts, it decreased rapid progressive population seminal oxidation-reduction potential < Conclusions protective effect on function together antimicrobial activity inborn presence body fluids, cervical mucus, makes enzyme agent preservation.

Язык: Английский

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Sow reproductive performance following artificial insemination with semen doses processed using Single Layer Centrifugation without antibiotics in the tropics

Theriogenology, Год журнала: 2024, Номер 226, С. 194 - 201

Опубликована: Июнь 21, 2024

Язык: Английский

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Evaluation of a Simple Antibiotic-Free Cryopreservation Protocol for Drone Semen

Insects, Год журнала: 2025, Номер 16(1), С. 50 - 50

Опубликована: Янв. 7, 2025

The increasing reliance of modern agriculture on honey bee (Apis mellifera) pollination has driven efforts to preserve and enhance populations. cryopreservation drone semen presents a promising solution for preserving genetic diversity supporting breeding programs without live animal transport risks. This study aimed evaluate one-step dilution antibiotic-free slow-freezing protocol under field conditions with in vitro vivo parameters. Semen viability was tested by two different mixes dyes, both techniques gave similar results, showing post-thaw drop 37%. Virgin queens were inseminated either fresh or frozen-thawed semen. Survival rates until egg-laying onset female brood production groups; however, colonies more likely go into wintering. Results suggest that can support viable queen insemination, potential even antibiotics the diluent. highlights need further refinement protocols, particularly regarding quality longevity, improve feasibility cryobanking Apis mellifera conservation breeding.

Язык: Английский

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Effect of Colloid Centrifugation and Cold Storage of Boar Semen at 4 °C on Bacterial Load and Sperm Quality

Antibiotics, Год журнала: 2025, Номер 14(3), С. 267 - 267

Опубликована: Март 6, 2025

Background/Objectives: Alternatives to antibiotics in semen extenders used for pig breeding are needed. Cold storage of boar is possible certain extenders; however, bacteria can still survive. Colloid centrifugation remove bacteria; therefore, a combination colloid and cold on the bacterial load sperm quality was investigated. Results: The original samples were similar with without antibiotics, respectively. At Day 4, count not different between controls stored at 17 °C SLC cold-stored antibiotics. proportion dead increased 16–18 (28.12 ± 21.72% vs. 32.88 20.94%; p < 0.05), DNA fragmentation from 1.5 1.02% 0 6.1 3.64% 4 (p 0.01), whereas it did deteriorate (Day 4: %DFI 2.00 1.65% 1.90 1.05% respectively). Methods: Aliquots ejaculates extended Androstar Premium processed by Single Layer Centrifugation (SLC) through Porcicoll; pellets resuspended AndroStar Premium, or cooled slowly, days °C. Controls (aliquots antibiotics) assessed after storage. Conclusions: combined an effective method reducing semen,

Язык: Английский

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Effects of Curcumin Oil Nano‐Emulsion on Buffalo Sperm Cryopreservation: Insights on Sperm Quality, Kinematic Parameters, Sperm Ultrastructure, Oxidative Stress, Apoptosis, Microbiota, and Molecular Docking Analysis and Fertilising Capacity

Reproduction in Domestic Animals, Год журнала: 2025, Номер 60(4)

Опубликована: Апрель 1, 2025

ABSTRACT This study investigated the potential of incorporating curcumin oil nano‐emulsion (CONE) into a Tris‐based freezing extender to relieve cryodamage effects and enhance sperm cryo‐resistance in buffalo bulls. Pooled semen from five fertile bulls ( n = 40 ejaculates) was divided supplemented with 0.5% CONE (CONE0.5), 1% (CONE1), or without (CONE0, control), then extended packaged. Post‐thaw assessments included kinematic parameters, oxidative stress markers, apoptosis levels, microbial load, ultrastructural integrity, molecular docking interactions, conception rates. The CONE0.5 group exhibited significantly higher viability, progressive motility, membrane as well lower nitric oxide MDA compared CONE0 CONE1 groups p < 0.05), indicating reduced stress. Compared group, percentage viable early apoptotic sperm, late 0.05). Both CONE‐treated demonstrated post‐thaw total bacterial, spore‐forming coliform bacterial counts Conception rates were (80.0%) (68.0%) > Molecular analysis revealed strong binding affinities between caspase‐3 (−7.87 kcal/mol), HSP70 (−8.52 PRDX‐1 (−8.90 suggesting mechanisms action. In summary, inclusion cryoprotective additive media demonstrates for improving quality possible enhancement reproductive efficiency.

Язык: Английский

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Innovative Approaches to Avoid Antibiotic Use in Equine Semen Cryopreservation: Advancing Sustainable Reproductive Technologies

Animals, Год журнала: 2025, Номер 15(10), С. 1368 - 1368

Опубликована: Май 9, 2025

This study evaluated the impact of different processing techniques on microbial load and sperm quality in frozen–thawed equine semen to identify alternatives reduce preventive use antibiotics. Semen was obtained processed under rigorous hygiene measures from ten stallions, using four protocols: Simple Centrifugation with antibiotics (S+) (S−), Filtration (F−) Single-Layer Colloidal (C−) an antibiotic-free extender. Microbial culture media, viability motility were assessed. results consistent across protocols, except Columbia 5% Sheep Blood Agar where S− exhibited higher than S+ (p < 0.05). However, F− C− showed similar loads S+. No significant differences observed progressive motility, average path velocity, straight-line velocity or wobble parameters between protocols. Total significantly compared other treatments Thus, regardless antibiotics, proposed methods achieved traditional antibiotic-inclusive protocol terms most relevant parameters. These findings suggest that C−, combined optimized measures, offers effective alternative prophylactic extenders.

Язык: Английский

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Artificial insemination of boar semen doses prepared with a low-density colloid under field conditions

Frontiers in Veterinary Science, Год журнала: 2025, Номер 12

Опубликована: Июнь 5, 2025

Introduction Bacterial contamination of ejaculates during semen collection is practically inevitable, and antibiotics are a constituent extenders. However, bacterial resistance to serious problem. The present study investigated the effect preparing by centrifugation using low-density colloid preparation doses under field conditions, as an alternative use antibiotics. Methods Five from four boars were each divided into two aliquots: control doses, which extended with commercial Beltsville Thawing Solution (BTS) containing (30 × 10 6 spermatozoa/mL), treated processed Porcicoll (300 g; 20 min); resulting sperm pellet was re-suspended in without spermatozoa/mL). Sperm motility kinematic variables assessed for computer-assisted analysis (CASA). Sows groups inseminated either or doses. In second round, after weaning litters first sows group those received For all groups, pregnancy rate, farrowing litter size, number live-born piglets, weaned piglets recorded. Results No differences ( p &gt; 0.05) between rounds observed any CASA-assessed variables. Pregnancy = 0.0271) 0.046) rates higher compared group. same both rounds. Under current experimental quality not impaired treatment Porcicoll. An interesting finding that able become pregnant again insemination lacking antibiotics, no on reproductive output. Conclusion conclusion, artificial (AI) boar Porcicoll, meeting modern requirements alternatives could be promising state-of-the-art approach.

Язык: Английский

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Antibióticos en medicina veterinaria y producción animal: resistencia y alternativas

South Florida Journal of Development, Год журнала: 2024, Номер 5(11), С. e4592 - e4592

Опубликована: Ноя. 7, 2024

Es indiscutible la importancia de los antibióticos (AB) para salud humana y animal, así como en producción alimentos origen animal. Sin embargo, su uso indiscriminado ha provocado que resistencia a AB (RAB) se convierta una crisis global puede alcanzar niveles catastróficos. Este desmedido es especialmente grave medicina veterinaria donde utilizan cerca del doble humanos, por lo requieren medidas efectivas controlar esta situación. Por ello, proponen alternativas sustituir aplicación terapéutica profiláctica, con resultados perspectivas variables. El concepto “Una Salud” busca concientizar al personal médico, población general, necesario actuar conjunto llevar cabo acciones contribuyan superar amenaza pública global. objetivo revisión fue resumir el conocimiento actual sobre las causas control RAB, impacto humana, animal ambiental.

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Boar semen microbiome: insights and potential implications

Animal Reproduction Science, Год журнала: 2024, Номер 272, С. 107647 - 107647

Опубликована: Ноя. 19, 2024

Язык: Английский

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