Trends in biotechnology,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 1, 2024
In
recent
decades,
the
field
of
synthetic
biology
has
witnessed
remarkable
progress,
driving
advances
in
both
research
and
practical
applications.
One
pivotal
area
development
involves
design
transgene
switches
capable
precisely
regulating
specified
outputs
controlling
cell
behaviors
response
to
physical
cues,
which
encompass
light,
magnetic
fields,
temperature,
mechanical
forces,
ultrasound,
electricity.
this
review,
we
delve
into
cutting-edge
progress
made
physically
controlled
protein
expression
engineered
mammalian
cells,
exploring
diverse
genetic
tools
strategies
available
for
engineering
targeting
cells
sense
these
cues
generate
desired
accordingly.
We
discuss
precision
efficiency
limitations
inherent
tools,
while
also
highlighting
their
immense
potential
therapeutic
Chemical Reviews,
Год журнала:
2018,
Номер
118(21), С. 10659 - 10709
Опубликована: Июль 9, 2018
Sensory
photoreceptors
underpin
light-dependent
adaptations
of
organismal
physiology,
development,
and
behavior
in
nature.
Adapted
for
optogenetics,
sensory
become
genetically
encoded
actuators
reporters
to
enable
the
noninvasive,
spatiotemporally
accurate
reversible
control
by
light
cellular
processes.
Rooted
a
mechanistic
understanding
natural
photoreceptors,
artificial
with
customized
light-gated
function
have
been
engineered
that
greatly
expand
scope
optogenetics
beyond
original
application
light-controlled
ion
flow.
As
we
survey
presently,
UV/blue-light-sensitive
particularly
allowed
transcend
its
initial
neuroscience
applications
unlocking
numerous
additional
processes
parameters
optogenetic
intervention,
including
gene
expression,
DNA
recombination,
subcellular
localization,
cytoskeleton
dynamics,
intracellular
protein
stability,
signal
transduction
cascades,
apoptosis,
enzyme
activity.
The
engineering
novel
benefits
from
powerful
reusable
design
strategies,
most
importantly
association
(un)folding
reactions.
Additionally,
modified
versions
these
same
serve
as
fluorescent
proteins
generators
singlet
oxygen,
thereby
further
enriching
toolkit.
available
upcoming
detailed
quantitative
interrogation
networks
increasingly
more
precise
illuminating
manners.
Frontiers in Molecular Biosciences,
Год журнала:
2015,
Номер
2
Опубликована: Май 12, 2015
The
Light-Oxygen-Voltage
domain
family
of
proteins
is
widespread
in
biology
where
they
impart
sensory
responses
to
signal
transduction
domains.
small,
light
responsive
LOV
modules
offer
a
novel
platform
for
the
construction
optogenetic
tools.
Currently,
design
and
implementation
these
devices
partially
hindered
by
lack
understanding
how
drives
allosteric
changes
protein
conformation
activate
diverse
Further,
divergent
photocycle
properties
amongst
members
complicate
highly
sensitive
with
fast
on/off
kinetics.
In
present
review
we
discuss
history
research
primary
emphasis
on
tuning
chemistry
allow
improved
Chemical Reviews,
Год журнала:
2022,
Номер
123(5), С. 2349 - 2419
Опубликована: Дек. 13, 2022
Recent
advances
in
synthetic
biology
and
materials
science
have
given
rise
to
a
new
form
of
materials,
namely
engineered
living
(ELMs),
which
are
composed
matter
or
cell
communities
embedded
self-regenerating
matrices
their
own
artificial
scaffolds.
Like
natural
such
as
bone,
wood,
skin,
ELMs,
possess
the
functional
capabilities
organisms,
can
grow,
self-organize,
self-repair
when
needed.
They
also
spontaneously
perform
programmed
biological
functions
upon
sensing
external
cues.
Currently,
ELMs
show
promise
for
green
energy
production,
bioremediation,
disease
treatment,
fabricating
advanced
smart
materials.
This
review
first
introduces
dynamic
features
systems
potential
developing
novel
We
then
summarize
recent
research
progress
on
emerging
design
strategies
from
both
perspectives.
Finally,
we
discuss
positive
impacts
promoting
sustainability
key
future
directions.
Physiological Reviews,
Год журнала:
2022,
Номер
102(3), С. 1263 - 1325
Опубликована: Янв. 24, 2022
Optogenetics
combines
light
and
genetics
to
enable
precise
control
of
living
cells,
tissues,
organisms
with
tailored
functions.
has
the
advantages
noninvasiveness,
rapid
responsiveness,
tunable
reversibility,
superior
spatiotemporal
resolution.
Following
initial
discovery
microbial
opsins
as
light-actuated
ion
channels,
a
plethora
naturally
occurring
or
engineered
photoreceptors
photosensitive
domains
that
respond
at
varying
wavelengths
ushered
in
next
chapter
optogenetics.
Through
protein
engineering
synthetic
biology
approaches,
genetically
encoded
photoswitches
can
be
modularly
into
scaffolds
host
cells
myriad
biological
processes,
well
behavioral
disease
intervention
vivo.
Here,
we
summarize
these
optogenetic
tools
on
basis
their
fundamental
photochemical
properties
better
inform
chemical
design
principles.
We
also
highlight
exemplary
applications
opsin-free
optogenetics
dissecting
cellular
physiology
(designated
"optophysiology")
describe
current
progress,
future
trends,
wireless
optogenetics,
which
enables
remote
interrogation
physiological
processes
minimal
invasiveness.
This
review
is
anticipated
spark
novel
thoughts
next-generation
devices
promise
accelerate
both
basic
translational
studies.
Annual Review of Plant Biology,
Год журнала:
2012,
Номер
63(1), С. 49 - 72
Опубликована: Май 3, 2012
Photoreceptor
flavoproteins
of
the
LOV,
BLUF,
and
cryptochrome
families
are
ubiquitous
among
three
domains
life
configured
as
UVA/blue-light
systems
not
only
in
plants-their
original
arena-but
also
prokaryotes
microscopic
algae.
Here,
we
review
these
proteins'
structure
function,
their
biological
roles,
evolution
impact
living
world,
underline
growing
application
biotechnologies.
We
present
novel
developments
such
interplay
light
redox
stimuli,
emerging
enzymatic
functions,
lessons
on
from
picoalgae,
metagenomics
analysis,
optogenetics
applications.
