Why prokaryotes have pangenomes

Nature Microbiology, Год журнала: 2017, Номер 2(4)

Опубликована: Март 27, 2017

Язык: Английский

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Plant Microbiome Engineering: Expected Benefits for Improved Crop Growth and Resilience

Trends in biotechnology, Год журнала: 2020, Номер 38(12), С. 1385 - 1396

Опубликована: Май 22, 2020

Язык: Английский

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Inordinate Fondness Multiplied and Redistributed: the Number of Species on Earth and the New Pie of Life

The Quarterly Review of Biology, Год журнала: 2017, Номер 92(3), С. 229 - 265

Опубликована: Авг. 24, 2017

The number of species on Earth is one the most fundamental numbers in science, but that remains highly uncertain. Clearly, more exist than present formally described (approximately 1.5 million), projected differ dramatically among studies. Recent estimates range from about 2 million to approximately 1 trillion, project around 11 or fewer. Numerous studies have focused insects as a major component overall richness, and many excluded other groups, especially non-eukaryotes. Here, we re-estimate global biodiversity. We also estimate relative richness clades living organisms, summarized “Pie Life.” Unlike previous estimates, incorporate morphologically cryptic arthropod molecular-based delimitation. include numerous groups organisms not been simultaneously included those often associated with particular insect host (including mites, nematodes, apicomplexan protists, microsporidian fungi, bacteria). Our suggest there are likely be at least 6 billion Earth. Furthermore, contrast new Pie Life dominated by bacteria 70–90% species) only hyperdiverse groups.

Язык: Английский

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Oxford Nanopore R10.4 long-read sequencing enables the generation of near-finished bacterial genomes from pure cultures and metagenomes without short-read or reference polishing

Nature Methods, Год журнала: 2022, Номер 19(7), С. 823 - 826

Опубликована: Июль 1, 2022

Long-read Oxford Nanopore sequencing has democratized microbial genome and enables the recovery of highly contiguous genomes from isolates or metagenomes. However, to obtain near-finished it been necessary include short-read polishing correct insertions deletions derived homopolymer regions. Here, we show that R10.4 can be used generate metagenomes without reference polishing.

Язык: Английский

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Community Assembly Processes of the Microbial Rare Biosphere

Trends in Microbiology, Год журнала: 2018, Номер 26(9), С. 738 - 747

Опубликована: Март 14, 2018

Язык: Английский

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