Advanced Functional Materials,
Год журнала:
2023,
Номер
33(22)
Опубликована: Март 6, 2023
Abstract
Spray‐induced
gene
silencing
(SIGS)
represents
an
attractive
avenue
for
plant
protection,
but
limited
uptake
efficiency
of
double‐stranded
RNA
(dsRNA)
has
restricted
its
application
against
the
notorious
oomycetes,
Phytophthora
.
To
control
,
dsRNAs
targeting
two
essential
genes
in
are
screened
their
ability
to
partially
decrease
capsici
(
P.
)
infection
and
fecundity.
further
facilitate
SIGS
control,
functionalized
carbon
dots
(CDs)
complexed
with
(dsRNA‐CDs)
through
electrostatic
interaction.
dsRNA‐CDs
significantly
enhanced
efficacy
dsRNA
infestans
sojae
both
wild‐type
fungicide‐resistant
The
synergism
is
based
on
enhancing
stability
internalization.
Dual
treatment
corresponding
fungicide
reduced
amount
required
achieve
same
protective
effect
by
90%.
Plant
RdRP1
main
effector
processing
various
lengths
small
RNAs
induce
translation
inhibition
Notably,
here
first
a
nano‐delivery
system
improve
pathogens
reported.
Moreover,
elucidation
how
CD
facilitates
internalization
recipient
cell
molecular
mechanism
can
benefit
other
plant‐pathogen
pathosystems
improving
bioactivity
reducing
chemical
fungicides
application.
Chemical Reviews,
Год журнала:
2019,
Номер
119(22), С. 11631 - 11717
Опубликована: Окт. 1, 2019
Development
of
biosensing
platforms
plays
a
key
role
in
research
settings
for
identification
biomarkers
and
clinical
applications
diagnostics.
Biosensors
based
on
nucleic
acids
have
taken
many
forms,
from
simple
duplex-based
constructs
to
stimuli-responsive
acid
nanostructures.
In
this
review,
we
look
at
various
acid-based
biosensors,
the
different
read-out
strategies
employed,
their
use
chemical
biological
sensing.
We
also
current
developments
DNA
nanotechnology-based
biosensors
how
rational
design
such
leads
more
efficient
platforms.
PLANT PHYSIOLOGY,
Год журнала:
2019,
Номер
182(1), С. 38 - 50
Опубликована: Июль 8, 2019
Since
its
discovery
more
than
20
years
ago,
RNA
interference
(RNAi)
has
been
extensively
used
in
crop
protection
platforms.
So
far,
RNAi
approaches
have
conventionally
based
on
the
use
of
transgenic
plants
expressing
double
stranded
RNAs
(dsRNAs)
against
selected
targets.
However,
transgenes
and
genetically
modified
organisms
(GMOs)
raised
considerable
scientific
public
concerns.
Hence
emerged
need
for
alternative
that
avoid
resort
instead
to
direct
exogenous
application
molecules
potential
trigger
RNAi.
Here,
we
highlight
most
important
advances
this
field,
discussing
various
methods
delivery
diverse
targets,
such
as
plant
genes,
viruses,
viroids,
fungi,
insects,
mites,
nematodes.
In
addition,
examine
possible
shortcomings
these
methods,
underline
critical
parameters
be
met
a
desired
outcome,
explore
feasible
possibilities
increase
their
efficiency
applicability,
even
bacterial
pathogens.
PLANT PHYSIOLOGY,
Год журнала:
2020,
Номер
184(2), С. 647 - 657
Опубликована: Авг. 6, 2020
The
initiation
of
RNA
interference
(RNAi)
by
topically
applied
small
interfering
has
potential
applications
for
plant
functional
genomics,
crop
improvement
and
protection,
but
the
primary
obstacle
development
this
technology
is
efficient
delivery
RNAi
effectors
into
cell.
cell
wall
a
particularly
challenging
barrier
macromolecules
because
many
transfection
agents
that
are
commonly
used
with
animal
cells
produce
nanocomplexes
significantly
larger
than
size
exclusion
limit
wall.
Here,
we
illustrate
use
class
very
nanoparticles,
called
carbon
dots,
delivering
model
plants
Nicotiana
benthamiana
tomato
(Solanum
lycopersicum).
Low-pressure
spray
application
these
formulations
spreading
surfactant
resulted
in
strong
silencing
GFP
transgenes
both
species.
efficacy
dot
was
also
demonstrated
endogenous
genes
encode
two
subunits
magnesium
chelatase,
an
enzyme
necessary
chlorophyll
synthesis.
visible
phenotypes
observed
dot-facilitated
were
validated
measuring
significant
reductions
target
gene
transcript
and/or
protein
levels.
Methods
plants,
such
as
described
here,
could
become
valuable
tools
practical
genomics
agriculture.
