Animals,
Год журнала:
2024,
Номер
14(7), С. 1074 - 1074
Опубликована: Апрель 1, 2024
As
one
of
the
most
important
aquaculture
species
in
world,
improvement
growth
traits
Pacific
white
shrimp
(Litopenaeus
vannamei),
has
always
been
a
primary
focus.
In
this
study,
we
conducted
SNP-specific
locus
analysis
and
identified
growth-related
gene,
BAMBI,
L.
vannamei.
We
analyzed
structure
function
LvBAMBI
using
genomic,
transcriptomic,
metabolomic,
RNA
interference
(RNAi)
assays.
The
possessed
highly
conserved
structural
domains
widely
expressed
various
tissues.
Knockdown
significantly
inhibited
gain
body
length
weight
shrimp,
underscoring
its
role
as
growth-promoting
factor.
Specifically,
knockdown
resulted
significant
downregulation
genes
involved
lipid
metabolism,
protein
synthesis,
catabolism
transport,
immunity.
Conversely,
related
to
glucose
metabolism
exhibited
upregulations.
Analysis
differential
metabolites
(DMs)
metabolomics
further
revealed
that
may
primarily
affect
by
regulating
biological
processes
metabolism.
These
results
suggested
plays
crucial
transport
shrimp.
This
study
provides
valuable
insights
for
future
research
utilization
BAMBI
crustaceans.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(10), С. 8587 - 8587
Опубликована: Май 11, 2023
The
plant
mirid
bug
Apolygus
lucorum
is
an
omnivorous
pest
that
can
cause
considerable
economic
damage.
steroid
hormone
20-hydroxyecdysone
(20E)
mainly
responsible
for
molting
and
metamorphosis.
adenosine
monophosphate-activated
protein
kinase
(AMPK)
intracellular
energy
sensor
regulated
by
20E,
its
activity
allosterically
through
phosphorylation.
It
unknown
whether
the
20E-regulated
insect's
gene
expression
depends
on
AMPK
Herein,
we
cloned
full-length
cDNA
of
AlAMPK
in
A.
lucorum.
mRNA
was
detected
at
all
developmental
stages,
whereas
dominant
midgut
and,
to
a
lesser
extent,
epidermis
fat
body.
Treatment
with
20E
activator
5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside
(AlCAR)
or
only
AlCAR
resulted
activation
phosphorylation
levels
body,
probed
antibody
directed
against
phosphorylated
Thr172,
enhancing
expression,
no
occurred
compound
C.
Compared
C,
and/or
increased
rate,
fifth
instar
nymphal
weight
shortened
development
time
vitro
inducing
EcR-A,
EcR-B,
USP,
E75-A.
Similarly,
knockdown
RNAi
reduced
rate
nymphs,
fifth-instar
nymphs
blocked
20E-related
genes.
Moreover,
as
observed
TEM,
thickness
significantly
treatments,
spaces
began
form
between
cuticle
epidermal
cells,
progress
improved.
These
composite
data
indicated
AlAMPK,
pathway,
plays
important
role
hormonal
signaling
short,
regulating
insect
metamorphosis
switching
status.
Journal of Agricultural and Food Chemistry,
Год журнала:
2023,
Номер
71(30), С. 11491 - 11501
Опубликована: Июль 21, 2023
Bruceine
D
(BD)
is
an
effective
insecticidal
compound
found
in
the
Chinese
herb
Brucea
javanica
(L.)
Merr.
BD
inhibits
growth
and
metamorphosis
of
Plutella
xylostella
Drosophila
melanogaster;
however,
its
target
protein
molecular
mechanism
activity
remain
unclear.
In
this
study,
proteins
with
high
affinity
for
were
screened
using
surface
plasmon
resonance
high-performance
liquid
chromatography
coupled
matrix-assisted
laser
desorption/ionization-time-of-flight
mass
spectrometry,
revealing
ecdysone
receptor
(EcR)
main
BD.
vivo
results
showed
that
inhibited
insect
through
inhibition
expression
20E
response
genes.
vitro
dual
luciferase
enhanced
green
fluorescent
(EGFP)
fluorescence
experiments
indicated
suppressed
transcriptional
activation
EcR
by
blocking
element
(EcRE)-triggered
cascade,
suggesting
formation
20E–EcR–USP–EcRE
complex.
Moreover,
docking
demonstrated
bound
well
to
EcR.
Elucidating
will
be
helpful
development
pesticides
control
pests.
Proceedings of the National Academy of Sciences,
Год журнала:
2024,
Номер
121(3)
Опубликована: Янв. 8, 2024
Wing
dimorphism
of
insect
vectors
is
a
determining
factor
for
viral
long-distance
dispersal
and
large-area
epidemics.
Although
plant
viruses
affect
the
wing
plasticity
vectors,
potential
underlying
molecular
mechanisms
have
seldom
been
investigated.
Here,
we
found
that
planthopper-vectored
rice
virus,
stripe
virus
(RSV),
specifically
induces
long-winged
morph
in
male
insects.
The
analysis
field
populations
demonstrated
ratios
insects
are
closely
associated
with
RSV
infection
regardless
titers.
A
planthopper-specific
testis-highly
expressed
gene,
Animals,
Год журнала:
2024,
Номер
14(7), С. 1074 - 1074
Опубликована: Апрель 1, 2024
As
one
of
the
most
important
aquaculture
species
in
world,
improvement
growth
traits
Pacific
white
shrimp
(Litopenaeus
vannamei),
has
always
been
a
primary
focus.
In
this
study,
we
conducted
SNP-specific
locus
analysis
and
identified
growth-related
gene,
BAMBI,
L.
vannamei.
We
analyzed
structure
function
LvBAMBI
using
genomic,
transcriptomic,
metabolomic,
RNA
interference
(RNAi)
assays.
The
possessed
highly
conserved
structural
domains
widely
expressed
various
tissues.
Knockdown
significantly
inhibited
gain
body
length
weight
shrimp,
underscoring
its
role
as
growth-promoting
factor.
Specifically,
knockdown
resulted
significant
downregulation
genes
involved
lipid
metabolism,
protein
synthesis,
catabolism
transport,
immunity.
Conversely,
related
to
glucose
metabolism
exhibited
upregulations.
Analysis
differential
metabolites
(DMs)
metabolomics
further
revealed
that
may
primarily
affect
by
regulating
biological
processes
metabolism.
These
results
suggested
plays
crucial
transport
shrimp.
This
study
provides
valuable
insights
for
future
research
utilization
BAMBI
crustaceans.
