Broad scale proteomic analysis of heat-destabilised symbiosis in the hard coral Acropora millepora DOI Creative Commons
Katherina Petrou, Brook L. Nunn, Matthew P. Padula

и другие.

Scientific Reports, Год журнала: 2021, Номер 11(1)

Опубликована: Сен. 24, 2021

Abstract Coral reefs across the globe are threatened by warming oceans. The last few years have seen worst mass coral bleaching events recorded, with more than one quarter of all irreversibly impacted. Considering widespread devastation, we need to increase our efforts understanding physiological and metabolic shifts underlying breakdown this important symbiotic ecosystem. Here, investigated proteome (PRIDE accession # PXD011668) both host symbionts reef-building Acropora millepora exposed ambient (~ 28 °C) elevated temperature 32 °C for 2 days, following a five-day incremental increase) explored associated biomolecular changes in symbiont, aim gaining new insights into mechanisms underpinning collapse symbiosis. We identified 1,230 unique proteins (774 456 symbiont) control thermally stressed corals, which 107 significantly increased 125 decreased abundance under relative control. Proteins involved oxidative stress proteolysis constituted 29% that abundance, evidence impairment endoplasmic reticulum cytoskeletal regulation proteins. In detected decrease responsible photosynthesis energy production (33% abundance), yet minimal signs or proteolysis. Lipid stores > twofold despite reduction photosynthesis, suggesting reduced translocation carbon host. There were significant related state, including linked nitrogen metabolism V-ATPase (-0.6 fold change) known symbiosome acidity. These results highlight key differences symbiont proteomic adjustments identify two directly bilateral nutrient exchange as potential indicators symbiosis breakdown.

Язык: Английский

Extending the natural adaptive capacity of coral holobionts DOI
Christian R. Voolstra, David J. Suggett, Raquel S. Peixoto

и другие.

Nature Reviews Earth & Environment, Год журнала: 2021, Номер 2(11), С. 747 - 762

Опубликована: Окт. 12, 2021

Язык: Английский

Процитировано

192
Coral microbiome manipulation elicits metabolic and genetic restructuring to mitigate heat stress and evade mortality DOI Creative Commons
Érika P. Santoro, Ricardo M. Borges, Josh L. Espinoza

и другие.

Science Advances, Год журнала: 2021, Номер 7(33)

Опубликована: Авг. 13, 2021

Probiotics mitigate post-heat stress disorder, preventing coral mortality.

Язык: Английский

Процитировано

188
A stony coral cell atlas illuminates the molecular and cellular basis of coral symbiosis, calcification, and immunity DOI Creative Commons
Shani Levy, Anamaria Elek, Xavier Grau‐Bové

и другие.

Cell, Год журнала: 2021, Номер 184(11), С. 2973 - 2987.e18

Опубликована: Май 1, 2021

Stony corals are colonial cnidarians that sustain the most biodiverse marine ecosystems on Earth: coral reefs. Despite their ecological importance, little is known about cell types and molecular pathways underpin biology of reef-building corals. Using single-cell RNA sequencing, we define over 40 across life cycle Stylophora pistillata. We discover specialized immune cells, uncover developmental gene expression dynamics calcium-carbonate skeleton formation. By simultaneously measuring transcriptomes cells algae within them, characterize metabolic programs involved in symbiosis both partners. also trace evolution these specializations by phylogenetic integration multiple cnidarian type atlases. Overall, this study reveals cellular basis stony biology.

Язык: Английский

Процитировано

174
The evolution of the concept of stress and the framework of the stress system DOI Creative Commons

Siyu Lu,

Fang Wei, Guolin Li

и другие.

Cell Stress, Год журнала: 2021, Номер 5(6), С. 76 - 85

Опубликована: Май 31, 2021

Stress is a central concept in biology and has now been widely used psychological, physiological, social, even environmental fields. However, the of stress was cross-utilized to refer different elements system including stressful stimulus, stressor, response, effect. Here, we summarized evolution framework system. We find although developed from Selye's "general adaptation syndrome", it expanded evolved significantly. defined as state homeostasis being challenged, both local stress. A specific stressor may potentially bring about stress, while intensity beyond threshold commonly activate hypothalamic-pituitary-adrenal axis result systematic response. The indicates that includes three types: sustress (inadequate stress), eustress (good distress (bad stress). Both might impair normal physiological functions lead pathological conditions, benefit health through hormesis-induced optimization homeostasis. Therefore, an optimal level essential for building biological shields guarantee life processes.

Язык: Английский

Процитировано

144
Coral‐bleaching responses to climate change across biological scales DOI
Robert van Woesik, Tom Shlesinger, Andréa G. Grottoli

и другие.

Global Change Biology, Год журнала: 2022, Номер 28(14), С. 4229 - 4250

Опубликована: Апрель 27, 2022

Abstract The global impacts of climate change are evident in every marine ecosystem. On coral reefs, mass bleaching and mortality have emerged as ubiquitous responses to ocean warming, yet one the greatest challenges this epiphenomenon is linking information across scientific disciplines spatial temporal scales. Here we review some seminal recent coral‐bleaching discoveries from an ecological, physiological, molecular perspective. We also evaluate which data processes can improve predictive models provide a conceptual framework that integrates measurements biological Taking integrative approach scales, using for example hierarchical estimate major coral‐reef processes, will not only rapidly advance science but necessary guide decision‐making conservation efforts. To conserve encourage implementing mesoscale sanctuaries (thousands km 2 ) transcend national boundaries. Such networks protected reefs reef connectivity, through larval dispersal transverse thermal environments, genotypic repositories may become essential units selection environmentally diverse locations. Together, multinational be best chance corals persist change, while humanity struggles reduce emissions greenhouse gases net zero.

Язык: Английский

Процитировано

108
Coral holobiont cues prime Endozoicomonas for a symbiotic lifestyle DOI Creative Commons
Claudia Pogoreutz, Clinton A. Oakley, Nils Rädecker

и другие.

The ISME Journal, Год журнала: 2022, Номер 16(8), С. 1883 - 1895

Опубликована: Апрель 20, 2022

Abstract Endozoicomonas are prevalent, abundant bacterial associates of marine animals, including corals. Their role in holobiont health and functioning, however, remains poorly understood. To identify potential interactions within the coral holobiont, we characterized novel isolate marisrubri sp. nov. 6c assessed its transcriptomic proteomic response to tissue extracts native host, Red Sea Acropora humilis. We show that stimulated differential expression genes putatively involved symbiosis establishment via modulation host immune by E. 6c, such as for flagellar assembly, ankyrins, ephrins, serpins. Proteome analyses revealed upregulated vitamin B1 B6 biosynthesis glycolytic processes cues. Our results suggest priming a symbiotic lifestyle involves immunity exchange essential metabolites with other members. Consequently, may play an important nutrient cycling therefore contribute health, acclimatization, adaptation.

Язык: Английский

Процитировано

85
The coral microbiome: towards an understanding of the molecular mechanisms of coral–microbiota interactions DOI Creative Commons
Amin R. Mohamed,

Michael A. Ochsenkühn,

Ahmed M Kazlak

и другие.

FEMS Microbiology Reviews, Год журнала: 2023, Номер 47(2)

Опубликована: Март 1, 2023

Corals live in a complex, multipartite symbiosis with diverse microbes across kingdoms, some of which are implicated vital functions, such as those related to resilience against climate change. However, knowledge gaps and technical challenges limit our understanding the nature functional significance complex symbiotic relationships within corals. Here, we provide an overview complexity coral microbiome focusing on taxonomic diversity functions well-studied cryptic microbes. Mining literature indicate that while corals collectively harbour third all marine bacterial phyla, known symbionts antagonists represent minute fraction this these taxa cluster into select genera, suggesting selective evolutionary mechanisms enabled bacteria gain niche holobiont. Recent advances research aimed at leveraging manipulation increase coral's fitness help mitigate heat stress-related mortality discussed. Then, insights potential through microbiota can communicate modify host responses examined by describing recognition patterns, microbially derived epigenome effector proteins gene regulation. Finally, power omics tools used study highlighted emphasis integrated host-microbiota multiomics framework understand underlying during change-driven dysbiosis.

Язык: Английский

Процитировано

58
The coral microbiome in sickness, in health and in a changing world DOI
Christian R. Voolstra, Jean‐Baptiste Raina, Melanie Dörr

и другие.

Nature Reviews Microbiology, Год журнала: 2024, Номер 22(8), С. 460 - 475

Опубликована: Март 4, 2024

Язык: Английский

Процитировано

53
Triggers, cascades, and endpoints: connecting the dots of coral bleaching mechanisms DOI Creative Commons

Joshua Helgoe,

Simon K. Davy, Virginia M. Weis

и другие.

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Год журнала: 2024, Номер 99(3), С. 715 - 752

Опубликована: Янв. 12, 2024

ABSTRACT The intracellular coral–dinoflagellate symbiosis is the engine that underpins success of coral reefs, one most diverse ecosystems on planet. However, breakdown and loss microalgal symbiont (i.e. bleaching) due to environmental changes are resulting in rapid degradation reefs globally. There an urgent need understand cellular physiology bleaching at mechanistic level help develop solutions mitigate reef crisis. Here, unprecedented scope, we present novel models integrate putative mechanisms within a common framework according triggers (initiators bleaching, e.g. heat, cold, light stress, hypoxia, hyposalinity), cascades (cellular pathways, photoinhibition, unfolded protein response, nitric oxide), endpoints (mechanisms loss, apoptosis, necrosis, exocytosis/vomocytosis). supported by direct evidence from cnidarian systems, indirectly through comparative evolutionary analyses non‐cnidarian systems. With this approach, new have been established between initiated different triggers. In particular, provide insights into poorly understood connections highlight role mechanism i.e. ‘symbiolysosomal digestion’, which symbiophagy. This review also increases approachability for specialists non‐specialists mapping vast landscape atlas comprehensible detailed models. We then discuss major knowledge gaps how future research may improve understanding cascade pathways (endpoints).

Язык: Английский

Процитировано

39
Fast and pervasive transcriptomic resilience and acclimation of extremely heat-tolerant coral holobionts from the northern Red Sea DOI Creative Commons
Romain Savary, Daniel J. Barshis, Christian R. Voolstra

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2021, Номер 118(19)

Опубликована: Май 3, 2021

Corals from the northern Red Sea and Gulf of Aqaba exhibit extreme thermal tolerance. To examine underlying gene expression dynamics, we exposed

Язык: Английский

Процитировано

89