Predicting nanocarriers’ efficacy in 3D models with Brillouin microscopy DOI Creative Commons
Giulia Guerriero, Alexis Viel,

Veronica Feltri

и другие.

Nanoscale, Год журнала: 2023, Номер 15(47), С. 19255 - 19267

Опубликована: Янв. 1, 2023

Thanks to their unique nanoscale properties, nanomedicines can overcome some of the shortcomings conventional therapies. For better predictive screening, it is important assess performance in three-dimensional (3D) multicellular tumour spheroids (MCTS) that recapitulate physiological barriers found real tumours. Today, evaluation drug delivery nanosystems MCTS mainly explored by means microscopy techniques are invasive and require fluorescent labels which modify composition fate carriers. In recent years, a new quantitative technique based on Brillouin light scattering (BLS) has been proposed uses interaction laser with picosecond timescale density fluctuations sample. Because label-free, all-optical non-destructive, BLS gained interest pharmaceutical biomedical fields. this work, we implemented fast spectrometer used frequency shift at center as readout for efficacy. We first investigated ability setup quantify efficacy grown classical multiwell plates concluded low number samples available multiwells limits statistical significance results. To improve throughput, then combined microscope agarose microwells designed fabricate large test 50 less than minute. Using platform, assessed polymeric nanoparticles (NPs) loaded platinum derivative anticancer (dichloro(1,2-diaminocyclohexane)platinum(II)) reducing growth colorectal cancer cells (HCT-116) MCTS. observe time- dose-dependent decrease shift, revealing progressive loss mechanical integrity These results demonstrate probing promising tool high-throughput screening nanocarriers 3D models.

Язык: Английский

Brillouin light scattering anisotropy microscopy for imaging the viscoelastic anisotropy in living cells DOI
Hamid Keshmiri, Domagoj Cikes, Marketa Samalova

и другие.

Nature Photonics, Год журнала: 2024, Номер 18(3), С. 276 - 285

Опубликована: Янв. 18, 2024

Язык: Английский

Процитировано

14
Strong Inhibition of Ice Growth by Biomimetic Crowding Coacervates DOI
Zhang Liu, Huimei Cao, Yaxun Fan

и другие.

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(38)

Опубликована: Авг. 3, 2023

The freezing of biological fluids is intensively studied but remains elusive as it affected not only by the various components also crowding nature fluids. Herein, we constructed spherical crowders, fibrous and coacervates ranging from surfactants to polymers proteins, mimic three typical crowders in fluids, i.e., globular networks, condensates biomolecules. It elucidated that exhibit low, moderate, strong ice growth inhibition activity, respectively, resulting their different abilities slowing down water dynamics. Intriguingly, coacervate consisting molecules without obvious activity strongly inhibits growth, which firstly employed a highly-potent cryoprotectant. This work provides new insights into survival freezing-tolerant organisms opens an avenue for design ice-controlling materials.

Язык: Английский

Процитировано

16
Probing molecular crowding in compressed tissues with Brillouin light scattering DOI Creative Commons
Guqi Yan, Sylvain Monnier, Malèke Mouelhi

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2022, Номер 119(4)

Опубликована: Янв. 19, 2022

Significance Volume regulation is key in maintaining important tissue functions, such as growth or healing. In this process, the role of efflux cellular fluids difficult to capture due lack apt technologies. Here, we use a tool based on Brillouin light scattering (BLS) that uses interaction laser with inherent picosecond timescale density fluctuations sample. We induced gradual volume decrease multicellular spheroids using osmotic perturbations. BLS revealed nonlinear increase compressibility subsequent increased biopolymer crowding within cells. Our findings should inspire research regarding and tissues stimulate emergence models for cell mechanics at short timescales.

Язык: Английский

Процитировано

15
Estimation of the mass density of biological matter from refractive index measurements DOI Creative Commons
Conrad Möckel, Timon Beck, Sara Kaliman

и другие.

Biophysical Reports, Год журнала: 2024, Номер 4(2), С. 100156 - 100156

Опубликована: Апрель 24, 2024

The quantification of physical properties biological matter gives rise to novel ways understanding functional mechanisms. One the basic biophysical is mass density (MD). It affects dynamics in sub-cellular compartments and plays a major role defining opto-acoustical cells tissues. As such, MD can be connected refractive index (RI) via well known Lorentz-Lorenz relation, which takes into account polarizability matter. However, computing based on RI measurements poses challenge, as it requires detailed knowledge biochemical composition sample. Here we propose methodology how for assumptions about sample respective measurements. To this aim, employ Biot mixing rule RIs alongside assumption volume additivity find an approximate relation RI. We use Monte-Carlo simulations Gaussian propagation uncertainty obtain analytical solutions uncertainties validate approach by applying set well-characterized complex mixtures given bovine milk intralipid emulsion estimate living zebrafish (Danio rerio) larvae trunk tissue. Our results illustrate importance implementing not only estimations but many other related problems, such mechanical using Brillouin microscopy transient optical coherence elastography.

Язык: Английский

Процитировано

3
Mechanical Evolution of Metastatic Cancer Cells in 3D Microenvironment DOI Creative Commons

Karlin Hilai,

Daniil Grubich,

Marcus Akrawi

и другие.

Small, Год журнала: 2025, Номер unknown

Опубликована: Март 21, 2025

Cellular biomechanics plays a critical role in cancer metastasis and tumor progression. Existing studies on cell are mostly conducted flat 2D conditions, where cells' behavior can differ considerably from those 3D physiological environments. Despite great advances developing vitro models, probing cellular elasticity conditions remains major challenge for existing technologies. In this work, optical Brillouin microscopy is utilized to longitudinally acquire mechanical images of growing cancerous spheroids over the period 8 days. The dense mapping enables us extract spatially resolved temporally evolving features that were previously inaccessible. Using an established machine learning algorithm, it demonstrated incorporating these extracted significantly improves classification accuracy cells, 74% 95%. Building finding, deep pipeline capable accurately differentiating normal ones solely using have been developed, suggesting cells potentially serve as new biomarker detection.

Язык: Английский

Процитировано

0
Mechanical evolution of metastatic cancer cells in three-dimensional microenvironment DOI

Karlin Hilai,

Daniil Grubich,

Marcus Akrawi

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 2, 2024

Cellular biomechanics plays critical roles in cancer metastasis and tumor progression. Existing studies on cell are mostly conducted flat 2D conditions, where cells' behavior can differ considerably from those 3D physiological environments. Despite great advances developing

Язык: Английский

Процитировано

2
Confined Brownian suspensions: Equilibrium diffusion, thermodynamics, and rheology DOI
Alp M. Sunol, Roseanna N. Zia

Journal of Rheology, Год журнала: 2023, Номер 67(2), С. 433 - 460

Опубликована: Янв. 23, 2023

We examine the impact of confinement on structure, dynamics, and rheology spherically confined macromolecular suspensions, with a focus role played by entropic forces, comparing limits strong hydrodynamics no hydrodynamics. present novel measurements osmotic pressure, intrinsic viscosity, long-time self-diffusivity in spherical find induces structural correlations restrictions configurational entropy that drive up pressure viscosity down self-diffusion. Even absence hydrodynamics, produces distinct short-time self-diffusion regimes. This finding revises previous understanding is purely hydrodynamic quantity. The proportional to an mobility, direct analog mobility. A caging plateau following regime stronger more durable without drift—a gradient volume fraction—drives particles out their cages. emerges when mobility arising from heterogeneous distribution particle drives local conclude gradients produce dynamical interactions weaken this effect. From statistical physics perspective, restricts entropy, driving (inverse) dynamics as tightens. support claim rescaling fraction distance confinement-dependent maximum packing, which collapses data for each rheological measure onto single curve.

Язык: Английский

Процитировано

5
The intriguing role of collagen on the rheology of cancer cell spheroids DOI Creative Commons
Daria Tsvirkun,

Jean Révilloud,

Arianna Giannetti

и другие.

Journal of Biomechanics, Год журнала: 2022, Номер 141, С. 111229 - 111229

Опубликована: Июль 28, 2022

Язык: Английский

Процитировано

8
Brillouin scattering from biomedical samples: the challenge of heterogeneity DOI Creative Commons
Martina Alunni Cardinali, Silvia Caponi, M. Mattarelli

и другие.

Journal of Physics Photonics, Год журнала: 2024, Номер 6(3), С. 035009 - 035009

Опубликована: Май 16, 2024

Abstract Brillouin light scattering (BLS), a non-destructive and non-contact technique, offers powerful tool for probing the micromechanical properties of biological tissues. However, inherent heterogeneity tissues can pose significant challenges in interpreting BLS spectra. In this study, we introduce novel method that harnesses intensity information within single spectrum to directly estimate Voigt average longitudinal modulus. Additionally, use determine ratio squared Pockels coefficients photoelastically heterogeneous samples, based on global analysis 2D map. This is shown effectively photoelastic soft hard components human bone tissues, enabling calculation elastic moduli. Furthermore, it has remarkable ability generate maps filling factor volume, shedding valuable intricate structure topography rough surfaces under mapping.

Язык: Английский

Процитировано

1
Biomechanical properties of metastatic breast cancer cells in high glucose and hyperosmolarity environment DOI Open Access
Wenjing Zou, Xinyu Zheng, Tao Chen

и другие.

Applied Physics Letters, Год журнала: 2023, Номер 122(11)

Опубликована: Март 13, 2023

Biomechanical properties of cancer cells have received increasing attention for diverse applications owing to their ability reveal the physiological and pathological states cells. The association between diabetes mellitus (DM) breast has been known a long time. However, specific mechanism remains be elucidated, especially at cellular level. This study observed alterations in biomechanical behavior MCF-10A, MCF-7, MDA-MB-231 under different glucose concentrations osmotic pressure levels. Experimental results suggested inverse correlation Young's modulus malignancy. high-glucose environment decreased metastatic reduced cell stiffness. hyperosmolarity had opposite effect. In diabetic patients, exhibited stronger deformability by upregulating sensitivity high concentration downregulating hyperosmolarity. These cytological features might promote invasiveness traverse tissue barriers achieve distant metastasis. We proposed possible explain why DM may risk factor from perspective biomechanics. work pave way optimizing diagnostics therapeutics with co-morbidities clinical practice.

Язык: Английский

Процитировано

3