A review on recent taxonomic updates of gut bacteria associated with social bees, with a curated genomic reference database

Insect Science, Год журнала: 2024, Номер unknown

Опубликована: Апрель 9, 2024

Honeybees and bumblebees play a crucial role as essential pollinators. The special gut microbiome of social bees is key factor in determining the overall fitness health host. Although harbor relatively simple microbial communities at genus level, recent studies have unveiled significant genetic divergence variations gene content within each bacterial genus. However, comprehensive refined genomics-based taxonomic database specific to bee microbiomes remains lacking. Here, we first provided an overview current knowledge on distribution function bacteria, well factors that influence population dynamics. We then consolidated all available genomes bacteria species-level taxonomy, by constructing maximum-likelihood core genome phylogeny calculating genome-wide pairwise average nucleotide identity. On basis species constructed curated genomic reference database, named microbe sequence (BGM-GDb). To evaluate species-profiling performance BGM-GDb, retrieved series metagenomic data inferred composition using intra-species diversity analysis system (MIDAS), compared results with those obtained from prebuilt MIDAS database. found default BGM-GDb excelled aligned read counts richness. Overall, this high-resolution precise will facilitate research understanding community structure bees.

Язык: Английский

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Identification of the mutual gliding locus as a factor for gut colonization in non-native bee hosts using the ARTP mutagenesis

Microbiome, Год журнала: 2024, Номер 12(1)

Опубликована: Май 23, 2024

Abstract Background The gut microbiota and their hosts profoundly affect each other’s physiology evolution. Identifying host-selected traits is crucial to understanding the processes that govern evolving interactions between animals symbiotic microbes. Current experimental approaches mainly focus on model bacteria, like hypermutating Escherichia coli or evolutionary changes of wild stains by host transmissions. A method called atmospheric room temperature plasma (ARTP) may overcome bottleneck low spontaneous mutation rates while maintaining mild conditions for bacteria. Results We established an system with gnotobiotic bee models unravel molecular mechanisms promoting colonization. By in vivo serial passage, we tracked genetic ARTP-treated Snodgrassella strains from Bombus terrestris non-native honeybee host. observed passaged isolates showing mutual gliding locus have a competitive advantage Specifically, alleles orphan mglB , GTPase activating protein, promoted colonization potentially altering type IV pili-dependent motility cells. Finally, competition assays confirmed mutations out-competed ancestral strain but not native Conclusions Using ARTP mutagenesis generate library symbionts, explored potential improved hosts. Our findings demonstrate implication cell mutual-gliding association provide future study host-microbe interactions.

Язык: Английский

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Temporospatial dynamics and host specificity of honeybee gut bacteria

Cell Reports, Год журнала: 2024, Номер 43(7), С. 114408 - 114408

Опубликована: Июнь 26, 2024

Honeybees are important pollinators worldwide, with their gut microbiota playing a crucial role in maintaining health. The bacteria of honeybees consist primarily five core lineages that spread through social interactions. Previous studies have provided basic understanding the composition and function honeybee microbiota, recent advancements focusing on analyzing diversity at strain level changes bacterial functional genes. Research across different regions globally has insights into microbial ecology. Additionally, findings shed light mechanisms host specificity bacteria. This review explores temporospatial dynamics discussing reasons behind these fluctuations. synopsis provides host-microbe interactions is invaluable for

Язык: Английский

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The adaptation of bumblebees to extremely high elevation associated with their gut microbiota

mSystems, Год журнала: 2024, Номер 9(3)

Опубликована: Фев. 8, 2024

ABSTRACT Bumblebees are among the most abundant and important pollinators for sub-alpine alpine flowering plant species in Northern Hemisphere, but little is known about their adaptations to high elevations. In this article, we focused on two bumblebee species, Bombus friseanus prshewalskyi , respective gut microbiota. The distributed through Hengduan Mountains of southwestern China, show replacement at different We performed genome sequencing based 20 worker bee samples each species. Applying evolutionary population genetics metagenomic approaches, detected genes under selection analyzed functional pathways between bumblebees microbes. found clear genetic differentiation host significant differences Species occurred both hosts bacteria ( Snodgrassella ) with an increase elevation. These extremely high-elevation evidence positive related diverse biological processes. Positively selected involved immune systems probably contributed microbiota changes, while butyrate generated by may influence energy metabolism systems. This suggests a close association genomes microbiomes some degree natural selection. IMPORTANCE Two closely dominant elevations showed genomic signature adaptation elevation molecular level Bumblebees’ higher associated processes: response. Information allowing us understand adaptive mechanisms extreme conditions implicit if conserve them as environments change.

Язык: Английский

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One-step genome engineering in bee gut bacterial symbionts

mBio, Год журнала: 2024, Номер 15(9)

Опубликована: Авг. 6, 2024

ABSTRACT Mechanistic understanding of interactions in many host-microbe systems, including the honey bee microbiome, is limited by a lack easy-to-use genome engineering approaches. To this end, we demonstrate one-step approach for making gene deletions and insertions chromosomes gut bacterial symbionts. Electroporation linear or non-replicating plasmid DNA containing an antibiotic resistance cassette flanked regions with homology to symbiont reliably results chromosomal integration. This lightweight does not require expressing any exogenous recombination machinery. The high concentrations large DNAs long needed make process efficient can be readily produced using modern synthesis assembly methods. We use knock out genes, genes involved biofilm formation, insert fluorescent protein into chromosome betaproteobacterial Snodgrassella alvi . are also able engineer genomes multiple strains S. another species, communis , which found bumble microbiome. Finally, same method symbiont, Bartonella apis alphaproteobacterium. As expected, knockout recA -dependent, suggesting that straightforward procedure applied other microbes convenient IMPORTANCE Honey bees ecologically economically important crop pollinators symbionts influence their health. Microbiome-based strategies studying improving health have utilized wild-type plasmid-engineered bacteria. straightforward, single-step used cassettes replace investigating mechanisms community stably benefit pollinator

Язык: Английский

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Bee Microbiomes: Unveiling Diversity and Social Dynamics in Managed and Wild Bees

Опубликована: Янв. 1, 2025

Язык: Английский

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Genomic divergence across the tree of life

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(10)

Опубликована: Фев. 27, 2025

Nucleotide sequence data are being harnessed to identify species, even in cases which organisms themselves neither hand nor witnessed. But how genome-wide divergence maps species status is far from clear. While gene commonly used delineate bacterial its correspondence established boundaries has yet be explored across eukaryotic taxa. Because the processes underlying flow differ fundamentally between prokaryotes and eukaryotes, these domains likely relationship reproductive isolation divergence. In prokaryotes, homologous recombination, basis of flow, depends directly on degree genomic divergence, whereas sexually reproducing incompatibility can stem changes very few genes. Guided by measures bacteria, we gauge criteria correspond eukaryotes. recognized levels within typically small, averaging <1% protein-coding regions most animals, plants, fungi. There instances sister same or less than that among conspecifics. contrast, defined as populations exchanging genes, show both considerably higher. Although no single threshold delineates with >1% separate prokaryotic 1% still able recombine thus considered species.

Язык: Английский

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Host specificity of gut microbiota associated with social bees: patterns and processes

Microbiology and Molecular Biology Reviews, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

SUMMARYGut microbes provide benefits to some animals, but their distribution and effects across diverse hosts are still poorly described. There is accumulating evidence for host specificity (i.e., a pattern where different tend associate with distinct lineages), the causes consequences of this unclear. Combining experimental tests in laboratory broad surveys wild promising approach gaining comprehensive mechanistic understanding prevalence, origin, importance. Social bees represent an ideal testbed endeavor because they phylogenetically functionally diverse, host-specific, stable, tractable gut microbiota. Furthermore, western honeybee (Apis mellifera) emerging model system studying microbiota-host interactions. In review, we summarize data on prevalence strength social bee microbiota (bumblebees, stingless bees, honeybees), as well potential proven ecological molecular mechanisms that maintain specificity. Overall, found relatively strong likely results from several processes, including filtering mediated by immune priority effects. However, more research needed multiple species confirm these findings. To help future research, hypotheses field propose comparative tests. Finally, conclude review highlighting need understand how can influence health.

Язык: Английский

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Host Specificity of Snodgrassella in Eastern and Western Honeybees and Its Effects on Naturally Occurring Deformed Wing Virus Titers

Insects, Год журнала: 2025, Номер 16(5), С. 478 - 478

Опубликована: Май 1, 2025

Honeybee gut microbiota undergoes long-term adaptive evolution with the host, resulting in host-specific genomic and functional characteristics. However, specific role of host-specialized strains shaping host physiological functions remains understudied. This study investigates specificity core symbiont Snodgrassella A. cerana mellifera, exploring its effects on immune response natural virus suppression through analysis colonization experiments. Genomic revealed that strain from exhibited a larger genome greater gene content compared to cerana. Competitive experiments showed although different origins had similar efficiency displayed clear home-field advantage competitive process. Moreover, Inoculation mellifera native significantly reduced Deformed Wing Virus (DWV) titers, whereas non-native no effect. In contrast, neither altered DWV or Sacbrood (SBV) levels Immune only upregulated defensin 2 while significant changes occurred either strain. These results suggest exhibits at level, influencing both suppression, showing efficacy these roles, especially mellifera.

Язык: Английский

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Honey bee functional genomics using symbiont-mediated RNAi

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Апрель 25, 2022

Abstract Bees are indispensable pollinators and model organisms for studying social behavior, development, cognition. However, their eusociality makes it difficult to use standard forward genetic approaches study gene function. To fill this gap, we engineered the bee gut bacterium Snodgrassella alvi induce a host RNA interference response that reduces expression of targeted gene. employ FUGUES ( FU nctional G enomics U sing E ngineered S ymbionts) procedure, double-stranded plasmid is cloned in Escherichia coli using Golden Gate assembly then transferred S. . Adult worker bees colonized with Finally, knockdown verified through qRT-PCR, phenotypes interest can be further assessed. Expression genes reduced by as much 50-75% throughout entire body five days after colonization. This protocol accomplished four weeks researchers microbiology molecular cloning skills.

Язык: Английский

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