Stem Cells and Development, Год журнала: 2023, Номер 32(21-22), С. 667 - 669
Опубликована: Ноя. 1, 2023
Язык: Английский
Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Апрель 29, 2024
Autologous skin grafting is a standard treatment for defects such as burns. No artificial substitutes are functionally equivalent to autologous grafts. The cultured epidermis lacks the dermis and does not engraft deep wounds. Although reconstituted skin, which consists of epidermal cells on synthetic dermal substitute, can wounds, it requires wound bed be well-vascularized appendages. In this study, we successfully generate complete grafts with pluripotent stem cell-derived appendages p63 knockout embryos' dermis. Donor keratinocytes encroach by eliminating based cell-extracellular matrix adhesion mediated cell competition. chimeric contains allogenic dermis, engraftable long Furthermore, could semi-humanized segments human injection into amnionic cavity mice embryos. Niche encroachment opens possibility graft production in livestock animals.
Язык: Английский
Процитировано
7
Genes, Год журнала: 2025, Номер 16(2), С. 215 - 215
Опубликована: Фев. 12, 2025
Solid organ transplantation remains a life-saving treatment for patients worldwide. Unfortunately, the supply of donor organs cannot meet current need, making search alternative sources even more essential. Xenotransplantation using sophisticated genetic engineering techniques to delete and overexpress specific genes in animal has been investigated as possible option. However, use exogenous tissue presents another host obstacles, particularly regarding rejection. Given these limitations, interspecies blastocyst complementation combination with precise gene knockouts unique, promising pathway transplant shortage. In recent years, great advancements have made field, encouraging results producing donor-derived chimeric host. That said, one major barriers successful chimerism is mismatch developmental stages cells embryo. Another barrier speeds between embryos. This review outlines 19 studies which was used generate solid organs. particular, genesis liver, lung, kidney, pancreas, heart, thyroid, thymus parathyroids investigated. Of studies, 7 included an model. 7, completed human pig host, all others were rat-mouse chimeras. While very demonstrated, several challenges continue persist. chimerism, generation contribution, synchronized donor-host development, well ethical concerns human-animal chimeras remain important aspects that will need be addressed future research.
Язык: Английский
Процитировано
0
Journal of Endocrine Surgery, Год журнала: 2025, Номер 25(1), С. 1 - 1
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Hepatic Medicine Evidence and Research, Год журнала: 2024, Номер Volume 16, С. 11 - 29
Опубликована: Фев. 1, 2024
Orthotopic liver transplantation (OLT) currently serves as the sole definitive treatment for thousands of patients suffering from end-stage disease; and existing supply donor livers OLT is drastically outpaced by increasing demand. To alleviate this significant gap in treatment, several experimental approaches have been devised with aim either offering interim support to waiting on transplant list or bioengineering complete infusing them fresh hepatic cells. Recently, interspecies blastocyst complementation has emerged a promising method generating organs utero over short timeframe. When coupled gene editing technology, it brought about potentially revolutionary transformation regenerative medicine. Blastocyst harbors notable potential human large animals, which could be used xenotransplantation humans, addressing scarcity OLT. Nevertheless, substantial ethical challenges still need overcome produce larger domestic animals like pigs. This review compiles current understanding outlines future possibilities humans.
Язык: Английский
Процитировано
3
Frontiers in Endocrinology, Год журнала: 2024, Номер 14
Опубликована: Янв. 16, 2024
Based on the results of retrospective studies, Vincentis et al. reported importance serum Ca/P ratio in early identification patients with pseudohypoparathyroidism (PHP) and hypoparathyroidism (HPT) [1]. This indicator is particularly valuable for diagnosing atypical phenotypes parathyroid dysfunction, such as asymptomatic mild hypocalcemia. Additionally, study found that type 1B (PHP1B) subgroup exhibited worse biochemical characteristics at diagnosis compared to those (PHP1A) subgroup, lower Ca higher PTH, resulting a ratio. These fill gap application PTH calcium diseases.
Язык: Английский
Процитировано
1
Juan Cuevas eBooks, Год журнала: 2024, Номер unknown
Опубликована: Май 13, 2024
El quinto volumen de la serie "Abordajes y Técnicas Quirúrgicas" presenta una exhaustiva recopilación los procedimientos quirúrgicos más relevantes en el ámbito medicina contemporánea. Este libro se erige como un recurso fundamental para profesionales salud que buscan adentrarse complejidad diversidad las técnicas quirúrgicas modernas.
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Май 7, 2024
A key goal of developmental biology is to determine the extent which cells and organs develop autonomously, as opposed requiring interactions with other or environmental factors. Chimeras have played a foundational role in this by enabling qualitative classification cell-intrinsically vs. extrinsically driven processes. Here, we extend framework precisely decompose evolutionary divergence any quantitative trait into cell-intrinsic, extrinsic, intrinsic-extrinsic interaction components. Applying thousands gene expression levels reciprocal rat-mouse chimeras, found that majority their attributable cell-intrinsic factors, though extrinsic factors also play an integral role. For example, rat-like extracellular environment up-regulates transcriptional regulator endoplasmic reticulum (ER) stress response some but not all cell types, turn strongly predicts up-regulation its target genes ER pathway whole. This effect seen at protein level, suggesting propagation through multiple regulatory levels. our cellular trait, neuronal differentiation, revealed complex intrinsic Finally, show imprinted are dramatically mis-expressed species-mismatched environments, mismatch between rapidly evolving mechanisms controlling imprinting may contribute barriers interspecies chimerism. Overall, conceptual opens new avenues investigate mechanistic basis processes across myriad traits multicellular organism.
Язык: Английский
Процитировано
0
Endocrinology and Metabolism, Год журнала: 2024, Номер 39(4), С. 552 - 558
Опубликована: Июнь 10, 2024
Patients with permanent hypoparathyroidism require lifelong treatment. Current replacement therapies sometimes have adverse effects (e.g., hypercalciuria and chronic kidney disease). Generating parathyroid glands (PTGs) from the patient’s own induced pluripotent stem cells (PSCs), transplantation of these PTGs, would be an effective treatment option. Multiple methods for generating PTGs PSCs been reported. One major trend is <i>in vitro</i> differentiation into PTGs. Another vivo</i> generation PSC-derived by injecting PTG-deficient embryos. This review discusses current achievements challenges in present future PTG regenerative medicine.
Язык: Английский
Процитировано
0
Stem Cells and Development, Год журнала: 2023, Номер 32(21-22), С. 667 - 669
Опубликована: Ноя. 1, 2023
Язык: Английский
Процитировано
0