Natural Product Reports, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
This review highlights the discovery of functional microbial natural products from diverse niches. Leveraging biosynthetic capabilities microbes in organismic interactions is crucial for tackling antimicrobial resistance.
Язык: Английский
Frontiers in Microbiology, Год журнала: 2024, Номер 14
Опубликована: Янв. 5, 2024
The imbalance of microbial composition and diversity in favor pathogenic microorganisms combined with a loss beneficial gut microbiota taxa results from factors such as age, diet, antimicrobial administration for different infections, other underlying medical conditions, etc. Probiotics are known their capacity to improve health by stimulating the indigenous microbiota, enhancing host immunity resistance infection, helping digestion, carrying out various functions. Concurrently, metabolites produced these microorganisms, termed postbiotics, which include compounds like bacteriocins, lactic acid, hydrogen peroxide, contribute inhibiting wide range bacteria. This review presents an update on using probiotics managing treating human diseases, including complications that may emerge during or after COVID-19 infection.
Язык: Английский
Процитировано
58
ACS Chemical Biology, Год журнала: 2024, Номер 19(2), С. 357 - 369
Опубликована: Янв. 31, 2024
Recent advances in sequencing techniques unveiled the vast potential of ribosomally synthesized and post-translationally modified peptides (RiPPs) encoded microbiomes. Class I lantibiotics such as nisin A, widely used a food preservative, have been investigated for their efficacy killing pathogens. However, impact nisin-like class on commensal bacteria residing human gut remains unclear. Here, we report six gut-derived that are close homologues nisin, four which novel. We applied an improved lantibiotic expression platform to produce purify these antimicrobial assays. determined minimal inhibitory concentration (MIC) against both Gram-positive pathogens commensals profiled resistance genes commensals. Structure–activity relationship (SAR) studies with analogs revealed key regions residues properties. Our characterization SAR could shed light future development lantibiotic-based therapeutics preservatives.
Язык: Английский
Процитировано
13
Microorganisms, Год журнала: 2025, Номер 13(3), С. 555 - 555
Опубликована: Фев. 28, 2025
This review highlights several basic problems associated with bacterial drug resistance, including the decreasing efficacy of commercially available antimicrobials as well related problem microbiome irregularity and dysbiosis. The article explains that this present situation is addressable through LAB species, such Streptococcus salivarius Ligilactobacillus salivarius, which are established synthesizers both broad- narrow-spectrum antimicrobials. sheer number produced by species breadth their biological effects, in terms bacteriostatic/bactericidal abilities immunomodulation, make them prime candidates for new probiotics antibiotics. Given ease molecules can be biochemically engineered fact many these compounds target evolutionarily constrained sites, it seems apparent producing organisms ought to looked at next generation robust dual action symbiotic drugs.
Язык: Английский
Процитировано
0
Nature Communications, Год журнала: 2025, Номер 16(1)
Опубликована: Март 28, 2025
The human microbiome produces diverse metabolites that influence host health, yet the chemical landscape of ribosomally synthesized and post-translationally modified peptides (RiPPs)-a versatile class bioactive compounds-remains underexplored. Here, we conduct a large-scale biosynthetic analysis 306,481 microbial genomes from human-associated microbiomes, uncovering broad array yet-to-be-discovered RiPPs. These RiPPs are distributed across various body sites but show specific enrichment in gut oral microbiome. Big data omics reveals numerous RiPP families inversely related to diseases, suggesting their potential protective effects on health. For proof principle study, apply synthetic-bioinformatic natural product (syn-BNP) approach chemically synthesize nine autoinducing (AIPs) for vitro ex vivo assay. Our findings reveal five AIPs effectively inhibit biofilm formation disease-associated pathogens. Furthermore, when testing microbiota mice with inflammatory bowel disease, observe two can regulate community reduce harmful species. highlight vast regulating communities maintaining emphasizing therapeutic development.
Язык: Английский
Процитировано
0
mSphere, Год журнала: 2025, Номер unknown
Опубликована: Май 8, 2025
ABSTRACT Biofilms are an important colonization mechanism employed by several microbial species to better establish themselves and monopolize the acquisition of resources across different environs. Some bacteria have evolved specialized metabolites that, when secreted, disrupt formation stability biofilms generated competing heterospecies, providing producing organism with ecological advantage. Soil-derived probable candidates for identification such compounds, given intense level competition that occurs within terrestrial ecosystem. The MS14 strain Burkholderia contaminans isolated from soil in Mississippi has previously been shown produce antimicrobial compounds like occidiofungin ornibactin. In this report, we demonstrate also produces 4-hydroxy-3-methyl-2-alkenylquinoline (HMAQ-7), alkaloid-based metabolite structurally similar others produced . HMAQ-7 was purified sufficient quantities enable elucidation its covalent structure evaluation biological effects. compound found possess a unique ability inhibit biofilm biosynthesis species, including opportunistic pathogens Staphylococcus haemolyticus saliva-derived multispecies biofilms. demonstrated modulate additional cellular behaviors Bacillus subtilis, motility sporulation, suggesting molecule is interspecies dynamics present many diverse microenvironments. IMPORTANCE study furthers our understanding structural complexity functions 2-alkyl-4(1H)-quinolone spp. Low micromolar concentrations HMAQ-7′ induced observable bacterial growth morphology differences. antibiofilm properties characterized will promote future investigations into possible applied roles. alter using may facilitate multitude environments, is, aquatic, soil, possibly during infection. HMAQ subvert potential competitor natural environments lung infections cystic fibrosis patients.
Язык: Английский
Процитировано
0
Chemical Science, Год журнала: 2024, Номер 15(24), С. 9266 - 9273
Опубликована: Янв. 1, 2024
The cyclization step of lanthionines is typically catalyzed by a LanC cyclase or domain. Here, we report LanC-free pathway for the formation an ll -MeLan residue and stereochemically diverse allo AviMeCys in triantimycins.
Язык: Английский
Процитировано
1
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown
Опубликована: Сен. 17, 2023
Abstract Recent advances in sequencing techniques unveiled the vast potential of ribosomally synthesized and post-translationally modified peptides (RiPPs) encoded microbiomes. Class I lantibiotics such as nisin A, widely used a food preservative, have been investigated for their efficacy killing pathogens. However, impact nisin-like class on commensal bacteria residing human gut remains unclear. Here, we report six gut-derived that are close homologs nisin, four which novel. We applied an improved lantibiotic expression platform to produce purify these antimicrobial assays. determined minimal inhibitory concentration (MIC) against both Gram-positive pathogens commensals, profiled resistance genes commensals. SAR studies with variants revealed key regions residues properties. Our characterization commensals could shed light future development lantibiotic-based therapeutics preservatives.
Язык: Английский
Процитировано
1
medRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Янв. 24, 2024
ABSTRACT Introduction Dental caries is a disease that affects billions of people, and involves high low genetic susceptibility phenotypes different causal subtypes. The randomized clinical trial Precaries-RCT will evaluate prevention in adolescents, customized according to cause risk. Here we describe the two nested Precaries studies for cost-efficient oral healthcare personalized dentistry. Methods analysis present basic adaptive protocol multicentre intervention study, It includes prescreening versus susceptibility, through self-performed sampling by mail up 2000 adolescents aimed orthodontic treatment at community clinics, which 520 are enrolled RCT. participants allocated into groups – group each assigned intensive or standard prevention. primary outcome % reduction increment, relative genotype, with outcomes measured using tactile visual methods, bitewing radiographs, photos, quantitative laser fluorescence. design allows determination incidence progression rates inclusion additional human microbiota biomarkers study subjects. Biological samples (e.g. swab DNA, whole parotid saliva, microbiota) questionnaire data collected. also outline Precaries-adolescent sample mining predictor therapeutic target genes Precaries-birth cohort implementation our findings childhood. Ethics dissemination Ethical approval was obtained from Swedish national board research ethics committee (Dnr 2020-02533). Informed consent be participant. disseminated public conference presentations publication peer-reviewed scientific journals. Trial registration number www.clinicaltrials.gov , NCT05600517 STRENGTHS AND LIMITATIONS OF THIS STUDY Caries classification risk, measurements Multicentre patients conditions representative Public Service clinics Sweden, facilitating implementation, though part population. Prospective an model shortened time development on available smooth tooth surfaces. Frequent follow-up enables termination individuals progression, may allow further shortening times, but reduced individual entire period. Consensus-based intensified self-care multiple repeated blocks, ensuring dose; design, allowing flexible time, outcomes, extensive profiling online multimodal machine learning. Synergizes Precaries-adolescence predictors therapeutics, dentition/childhood.
Язык: Английский
Процитировано
0
RSC Chemical Biology, Год журнала: 2024, Номер 5(10), С. 1060 - 1073
Опубликована: Янв. 1, 2024
Here we describe the structure, bioactivity, and action mechanism of bibacillin 1 – a two-component lantibiotic from Bacillus thuringiensis.
Язык: Английский
Процитировано
0
Pharmaceuticals, Год журнала: 2024, Номер 17(12), С. 1725 - 1725
Опубликована: Дек. 20, 2024
Oral diseases, both acute and chronic, of infectious or non-infectious etiology, represent some the most serious medical problems in dentistry. Data from literature increasingly indicate that changes oral microbiome, therefore, overgrowing pathological microflora, lead to a variety oral-localized conditions such as caries, gingivitis, periodontitis. In recent years, compelling research has been devoted use natural antimicrobial peptides therapeutic agents possible treatment diseases. This review focuses on potential ceragenins (CSAs), which are lipid analogs peptides, molecules for development new methods prevention Studies date ceragenins, with their spectrum multidirectional biological activities, including antimicrobial, tissue regeneration-stimulating, anti-inflammatory, immunomodulatory properties, strong candidates further formulations. However, many beneficial properties require confirmation experimental reproducing environment fully determine application potential. Their transition practical also requires more advanced testing these clinical trials, have only conducted limited numbers date.
Язык: Английский
Процитировано
0