Genome-wide expression QTL mapping reveals the highly dynamic regulatory landscape of a major wheat pathogen DOI Creative Commons
Leen Abraham, Daniel Croll

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июль 16, 2023

Abstract Background In agricultural ecosystems, outbreaks of diseases are frequent and pose a significant threat to food security. A successful pathogen undergoes complex well-timed sequence regulatory changes avoid detection by the host immune system, hence well-tuned gene regulation is essential for survival. However, extent which polymorphisms in population provide an adaptive advantage poorly understood. Results We used Zymoseptoria tritici , one most important pathogens wheat, generate genome-wide map polymorphism governing expression. investigated transcription levels 146 strains grown under nutrient starvation performed expression quantitative trait loci (eQTL) mapping. identified cis -eQTLs 65.3% all genes majority eQTL within 2kb upstream downstream start site (TSS). also show that different elements contributes disproportionally variation. Investigating categories, we found enrichment variants predicted be fungal pathogenesis but with comparatively low effect size, suggesting separate layer involving epigenetics. previously reported trait-associated SNPs populations frequently -regulatory neighboring implications architecture. Conclusions Overall, our study provides extensive evidence single segregate large-scale variation likely fuel rapid adaptation resistant hosts environmental change.

Язык: Английский

Epigenetic regulation on fungal disease affecting plant-based food: A review from the perspectives of host, pathogen and their interactions DOI
Yan-Ling Ren,

Linyan Feng,

Xin Xu

и другие.

Food Bioscience, Год журнала: 2025, Номер unknown, С. 106633 - 106633

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Epigenetic regulation of nuclear processes in fungal plant pathogens DOI Creative Commons

H. Martin Kramer,

David E. Cook, Michael Seidl

и другие.

PLoS Pathogens, Год журнала: 2023, Номер 19(8), С. e1011525 - e1011525

Опубликована: Авг. 3, 2023

Through the association of protein complexes to DNA, eukaryotic nuclear genome is broadly organized into open euchromatin that accessible for enzymes acting on DNA and condensed heterochromatin inaccessible. Chemical physical alterations chromatin may impact its organization functionality are therefore important regulators processes. Studies in various fungal plant pathogens have uncovered an between expression planta - induced genes pathogenicity. This review discusses chromatin-based regulation mechanisms as determined pathogen Verticillium dahliae relates importance epigenetic transcriptional other processes more pathogens.

Язык: Английский

Процитировано

8
Identification and Characterization of Novel Candidate Effector Proteins from Magnaporthe oryzae DOI Creative Commons
Di Liu,

Zhiqin Lun,

Ning Liu

и другие.

Journal of Fungi, Год журнала: 2023, Номер 9(5), С. 574 - 574

Опубликована: Май 15, 2023

The fungal pathogen Magnaporthe oryzae secretes a large number of effector proteins to facilitate infection, most which are not functionally characterized. We selected potential candidate genes from the genome M. oryzae, field isolate P131, and cloned 69 putative for functional screening. Utilizing rice protoplast transient expression system, we identified that four genes, GAS1, BAS2, MoCEP1 MoCEP2 induced cell death in rice. In particular, also Nicotiana benthamiana leaves through Agrobacteria-mediated gene expression. further six MoCEP3 MoCEP8, suppress flg22-induced ROS burst N. upon These were highly expressed at different stage after infection. successfully knocked out five MoCEP1, MoCEP2, MoCEP3, MoCEP5 MoCEP7. virulence tests suggested deletion mutants showed reduced on barley plants. Therefore, those play an important role pathogenicity.

Язык: Английский

Процитировано

6
Genome-wide expression QTL mapping reveals the highly dynamic regulatory landscape of a major wheat pathogen DOI Creative Commons
Leen Abraham, Daniel Croll

BMC Biology, Год журнала: 2023, Номер 21(1)

Опубликована: Ноя. 20, 2023

Abstract Background In agricultural ecosystems, outbreaks of diseases are frequent and pose a significant threat to food security. A successful pathogen undergoes complex well-timed sequence regulatory changes avoid detection by the host immune system; hence, well-tuned gene regulation is essential for survival. However, extent which polymorphisms in population provide an adaptive advantage poorly understood. Results We used Zymoseptoria tritici , one most important pathogens wheat, generate genome-wide map polymorphism governing expression. investigated transcription levels 146 strains grown under nutrient starvation performed expression quantitative trait loci (eQTL) mapping. identified cis -eQTLs 65.3% all genes majority eQTL within 2kb upstream downstream start site (TSS). also show that different elements contributes disproportionally variation. Investigating categories, we found enrichment variants predicted be fungal pathogenesis but with comparatively low effect size, suggesting separate layer involving epigenetics. previously reported trait-associated SNPs populations frequently -regulatory neighboring implications architecture. Conclusions Overall, our study provides extensive evidence single segregate large-scale variation likely fuel rapid adaptation resistant hosts environmental change.

Язык: Английский

Процитировано

3
Newly identified Pijx gene: a weapon against both seedling and panicle blast in rice DOI

Kongkong Mondal,

Roshan Kumar Singh, Manoj Prasad

и другие.

Plant Cell Reports, Год журнала: 2024, Номер 43(4)

Опубликована: Март 24, 2024

Язык: Английский

Процитировано

0
FolSas2 is a regulator of early effector gene expression during Fusarium oxysporum infection DOI
Limin Song, Yalei Wang,

Fahui Qiu

и другие.

New Phytologist, Год журнала: 2024, Номер unknown

Опубликована: Дек. 8, 2024

Summary Fusarium oxysporum f. sp. lycopersici ( Fol ) that causes a globally devastating wilt disease on tomato relies the secretion of numerous effectors to mount an infection, but how pathogenic fungus precisely regulates expression effector genes during plant invasion remains elusive. Here, using molecular and cellular approaches, we show histone H4K8 acetyltransferase FolSas2 is transcriptional regulator early gene in . Autoacetylation K269 represses K335 ubiquitination, preventing its degradation by 26S proteasome. During infection process, elevates acetylation differentially changing transcription itself FolSir1 deacetylase, leading specific accumulation enzyme at this stage. subsequently activates array genes, as consequence, invades successfully. These findings reveal regulatory mechanism via autoacetylation modifier fungal invasion.

Язык: Английский

Процитировано

0
Genome-wide expression QTL mapping reveals the highly dynamic regulatory landscape of a major wheat pathogen DOI Creative Commons
Leen Abraham, Daniel Croll

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июль 16, 2023

Abstract Background In agricultural ecosystems, outbreaks of diseases are frequent and pose a significant threat to food security. A successful pathogen undergoes complex well-timed sequence regulatory changes avoid detection by the host immune system, hence well-tuned gene regulation is essential for survival. However, extent which polymorphisms in population provide an adaptive advantage poorly understood. Results We used Zymoseptoria tritici , one most important pathogens wheat, generate genome-wide map polymorphism governing expression. investigated transcription levels 146 strains grown under nutrient starvation performed expression quantitative trait loci (eQTL) mapping. identified cis -eQTLs 65.3% all genes majority eQTL within 2kb upstream downstream start site (TSS). also show that different elements contributes disproportionally variation. Investigating categories, we found enrichment variants predicted be fungal pathogenesis but with comparatively low effect size, suggesting separate layer involving epigenetics. previously reported trait-associated SNPs populations frequently -regulatory neighboring implications architecture. Conclusions Overall, our study provides extensive evidence single segregate large-scale variation likely fuel rapid adaptation resistant hosts environmental change.

Язык: Английский

Процитировано

1