Abstract
Microbial
dissimilatory
sulfate
reduction
is
a
key
process
in
the
global
sulfur
and
carbon
cycles
anoxic
ecosystems.
In
this
anaerobic
respiration,
phosphorylated
reduced
to
sulfite,
which
further
DsrC‐trisulfide
by
sulfite
reductase
DsrAB.
DsrD
small
protein
that
acts
as
an
allosteric
activator
of
DsrAB,
increasing
efficiency
reduction.
Here,
we
report
detailed
study
its
interaction
with
Sequence
similarity
analyses
show
there
are
three
groups
organisms
reductive‐type
The
regions
involved
DsrD–DsrAB
activity‐promoting
effect
were
investigated
through
vitro
silico
studies,
including
mutations
conserved
residues.
results
reveal
β‐loop
interaction,
contributing
better
understanding
mechanism
action.
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research,
Год журнала:
2024,
Номер
1871(5), С. 119732 - 119732
Опубликована: Апрель 15, 2024
Iron-sulfur
clusters
serve
as
indispensable
cofactors
within
proteins
across
all
three
domains
of
life.
Fe/S
emerged
early
during
the
evolution
life
on
our
planet
and
biogeochemical
cycle
sulfur
is
one
most
ancient
important
element
cycles.
It
therefore
no
surprise
that
have
crucial
roles
in
multiple
steps
microbial
metabolism.
During
dissimilatory
oxidation
prokaryotes,
not
only
electron
carriers
several
steps,
but
also
perform
catalytic
roles,
including
unprecedented
reactions.
Two
cytoplasmic
enzyme
systems
oxidize
sulfane
to
sulfite
are
particular
interest
this
context:
The
rDsr
pathway
employs
reverse
acting
reductase
rDsrAB
its
key
enzyme,
while
sHdr
utilizes
polypeptides
resembling
HdrA,
HdrB
HdrC
subunits
heterodisulfide
from
methanogenic
archaea.
Both
pathways
involve
components
predicted
bind
unusual
noncubane
catalysts
for
formation
disulfide
or
sulfite.
Mapping
cluster
machineries
sulfur-oxidizing
prokaryote
tree
reveals
ISC,
SUF,
MIS
SMS
sufficient
meet
maturation
requirements
operation
pathways.
dependent
lipoate-binding
assembled
by
a
novel
pathway,
involving
two
Radical
SAM
proteins,
namely
LipS1
LipS2.
These
coordinate
sulfur-donating
auxiliary
atypical
patterns
cysteines
histidine
act
lipoyl
synthases
jointly
inserting
atoms
an
octanoyl
residue.
This
article
part
Special
Issue
entitled:
Biogenesis
Function
proteins.
Microbial Genomics,
Год журнала:
2024,
Номер
10(2)
Опубликована: Фев. 20, 2024
Many
environmentally
relevant
micro-organisms
cannot
be
cultured,
and
even
with
the
latest
metagenomic
approaches,
achieving
complete
genomes
for
specific
target
organisms
of
interest
remains
a
challenge.
Cable
bacteria
provide
prominent
example
microbial
ecosystem
engineer
that
is
currently
unculturable.
They
occur
in
low
abundance
natural
sediments,
but
due
to
their
capability
long-distance
electron
transport,
they
exert
disproportionately
large
impact
on
biogeochemistry
environment.
Current
available
marine
cable
are
highly
fragmented
incomplete,
hampering
elucidation
unique
electrogenic
physiology.
Here,
we
present
pipeline
combines
Nanopore
long-read
Illumina
short-read
shotgun
sequencing.
Starting
from
clonal
enrichment
bacterium,
recovered
circular
metagenome-assembled
genome
(5.09
Mbp
size),
which
represents
novel
bacterium
species
proposed
name
Candidatus
Electrothrix
scaldis.
The
closed
contains
1109
identified
genes,
including
key
metabolic
enzymes
not
previously
described
incomplete
bacteria.
We
examined
detail
factors
leading
closure.
Foremost,
native,
non-amplified
long
reads
crucial
resolve
many
repetitive
regions
within
bacteria,
by
analysing
whole
assembly,
found
strain
diversity
insights
approaches
presented
here
could
help
achieve
closure
other
keystone
complex
environmental
samples
at
abundance.
Microorganisms
play
vital
roles
in
sulfur
cycling
through
the
oxidation
of
elemental
and
reduction
sulfite.
These
metabolisms
are
catalyzed
by
dissimilatory
sulfite
reductases
(Dsr)
functioning
either
reductive
or
reverse,
oxidative
direction.
Dsr-mediated
is
an
ancient
metabolism
proposed
to
have
fueled
energy
some
Earth's
earliest
microorganisms,
whereas
believed
evolved
later
association
with
widespread
availability
oxygen
on
Earth.
Organisms
generally
carry
out
pathway,
yet
organisms
from
diverse
phyla
been
discovered
gene
combinations
that
implicate
them
both
pathways.
A
comprehensive
investigation
into
these
regarding
Dsr
currently
lacking.
Here,
we
selected
one
phyla,
metabolically
versatile
candidate
phylum
SAR324,
study
ecology
evolution
metabolism.
We
confirmed
SAR324
encode
genes
associated
Dsr,
both.
Comparative
analyses
other
Dsr-encoding
bacterial
archaeal
revealed
encoding
proteins
constrained
a
few
phyla.
Further,
DsrAB
sequences
genomes
belonging
phylogenetically
positioned
at
interface
between
well-defined
clades.
The
phylogenetic
context
dsr
content
points
evolutionary
transition
event
ultimately
gave
way
Together,
this
research
suggests
mixed
origins
oxidation.
Coatings,
Год журнала:
2024,
Номер
14(9), С. 1105 - 1105
Опубликована: Сен. 1, 2024
Microbial
metal
corrosion
has
become
an
important
topic
in
research,
which
is
one
of
the
main
causes
equipment
damage,
energy
loss,
and
economic
loss.
At
present,
research
on
microbial
focuses
characteristics
products,
environmental
conditions
affecting
corrosion,
measures
means
prevention,
etc.
In
contrast,
taxa
involved
their
specific
role
process,
electron
transfer
pathway
are
relatively
small.
This
paper
summarizes
mechanism
carbon
steel
caused
by
SRB,
including
cathodic
depolarization
theory,
acid
metabolite
biocatalytic
sulfate
reduction
mechanism.
Based
reversible
nature
biofilms
fact
that
electrons
must
pass
through
extracellular
polymers
layer
between
solid
electrode
cell,
this
three
types
electrochemical
mechanisms
modes
occurring
fuel
cells,
direct-contact
transfer,
conductive
bacterial
hair
proteins
or
nanowires,
shuttling
mediated
use
soluble
mediators.
Finally,
a
more
complete
due
to
SRB
presented:
goes
from
anode,
polymer
layer,
membrane,
periplasm,
intracellular
reach
cytoplasm
for
allosteric
reduction.
article
also
variety
complex
components
such
as
DNA,
quinoline
humic
acid,
iron
sulfide
(FeSX),
Fe3+,
etc.,
may
act
donor
provide
chain;
bioinduced
mineralization
occurs
biofilm
can
inhibit
it
be
used
development
green
inhibitors.
provides
theoretical
guidance
diagnosis,
prediction,
prevention
corrosion.
Microbial
sulfate
reduction
is
central
to
the
global
carbon
cycle
and
redox
evolution
of
Earth's
surface.
Tracking
activity
reducing
microorganisms
over
space
time
relies
on
a
nuanced
understanding
stable
sulfur
isotope
fractionation
in
context
biochemical
machinery
metabolism.
Here,
we
link
magnitude
isotopic
proteomic
metabolite
profiles
under
different
cellular
energetic
regimes.
When
energy
availability
limited,
cell-specific
respiration
rates
net
inversely
covary.
Beyond
S
values,
also
quantified
shifts
protein
expression,
abundances
composition
intracellular
metabolites,
lipid
structures
lipid/water
H
values.
These
coupled
approaches
reveal
which
shift
directly
as
function
flux,
those
that
vary
minimally,
may
independent
flux
likely
do
not
contribute
S-isotope
fractionation.
By
coupling
bulk
observations
with
quantitative
proteomics,
provide
novel
constraints
for
metabolic
models.
Together,
these
results
lay
foundation
more
predictive
models,
alongside
interpretations
environmental
reducer
lipid-H
data.
