Live imaging of paracrine signaling: Advances in visualization and tracking techniques
Cell Structure and Function,
Год журнала:
2025,
Номер
50(1), С. 1 - 14
Опубликована: Янв. 1, 2025
Live
imaging
techniques
have
revolutionized
our
understanding
of
paracrine
signaling,
a
crucial
form
cell-to-cell
communication
in
biological
processes.
This
review
examines
recent
advances
visualizing
and
tracking
factors
through
four
key
stages:
secretion
from
producing
cells,
diffusion
extracellular
space,
binding
to
target
activation
intracellular
signaling
within
cells.
Paracrine
factor
can
be
directly
visualized
by
fluorescent
protein
tagging
ligand,
or
indirectly
the
cleavage
transmembrane
pro-ligands
plasma
membrane
fusion
endosomes
comprising
factors.
Diffusion
has
been
studied
using
such
as
fluorescence
correlation
spectroscopy
(FCS),
recovery
after
photobleaching
(FRAP),
decay
photoactivation
(FDAP),
single-molecule
tracking.
Binding
cells
various
biosensors,
including
GPCR-activation-based
(GRAB)
sensors
Förster
resonance
energy
transfer
(FRET)
probes
for
receptor
tyrosine
kinases.
Finally,
is
monitored
biosensors
second
messengers,
transcription
factors,
so
on.
In
addition
tools,
also
highlights
emerging
optogenetic
chemogenetic
tools
triggering
release
which
essential
associating
outcomes
during
bioimaging
signaling.Key
words:
live
imaging,
optogenetics,
chemogenetics.
Язык: Английский
Tuning synapse strength by nanocolumn plasticity
Trends in Neurosciences,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Role of astroglia and microglia in Alzheimer's disease and multiple therapeutic interventions
Journal of Alzheimer s Disease,
Год журнала:
2025,
Номер
unknown
Опубликована: Июнь 2, 2025
Alzheimer's
disease
(AD)
is
characterized
by
deposition
of
amyloid-β
(Aβ)
and
neurofibrillary
tangles
(NFTs)
formed
aggregates
hyperphosphorylated
tau
proteins.
It
presents
a
formidable
global
health
challenge,
prompting
the
exploration
innovative
therapeutic
strategies.
This
review
aims
to
provide
thorough
discussion
astrocytes
microglia
examine
whether
they
are
overall
beneficial
or
detrimental
for
AD
on
level.
Based
this,
this
describes
treatment
solutions
that
likely
entail
manipulation
glial
cells
reduce
inflammation,
opting
boost
clearance
toxic
proteins,
thus
stabilizing
effects
AD.
These
entities,
inherent
central
nervous
system,
extend
their
functions
beyond
structural
support,
actively
engaging
in
various
physiological
pathological
processes
associated
with
Both
astroglia
contribute
significantly
neuroinflammatory
response
observed
Reactive
release
inflammatory
mediators,
while
activated
cytokines,
chemokines,
reactive
oxygen
species,
collectively
assisting
chronic
state
neuroinflammation.
Additionally,
partake
Aβ,
play
pivotal
role
phagocytosing
Aβ
plaques.
In
AD,
ongoing
inflammation
may
cause
buildup
which
causes
problems
also
worsens
these
issues
communication
between
neurons,
key
factor
cognitive
decline.
addition,
there
tremendous
opportunities
identify
new
biomarkers
specific
disorders,
genomic
epigenomic
approaches
selection
patients,
using
multimodal
imaging
techniques,
application
machine
learning
algorithms
future
personalized
glial-targeted
therapies.
Язык: Английский
Release your inhibitions: The cell biology of GABAergic postsynaptic plasticity
Current Opinion in Neurobiology,
Год журнала:
2024,
Номер
90, С. 102952 - 102952
Опубликована: Дек. 25, 2024
Язык: Английский
Trans-synaptic molecular context of NMDA receptor nanodomains
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 23, 2023
Tight
coordination
of
the
spatial
relationships
between
protein
complexes
is
required
for
cellular
function.
In
neuronal
synapses,
many
proteins
responsible
neurotransmission
organize
into
subsynaptic
nanoclusters
whose
trans-cellular
alignment
modulates
synaptic
signal
propagation.
However,
these
and
NMDA
receptors
(NMDARs),
which
are
learning
memory,
remain
undefined.
Here,
we
mapped
relationship
key
NMDAR
subunits
to
reference
in
active
zone
postsynaptic
density
using
multiplexed
super-resolution
DNA-PAINT
microscopy.
GluN2A
GluN2B
formed
with
diverse
configurations
that,
surprisingly,
were
not
localized
near
presynaptic
vesicle
release
sites
marked
by
Munc13-1.
a
subset
was
configured
maintain
activation:
internally
denser,
aligned
abundant
PSD-95,
associated
closely
specific
nanodomains.
This
work
reveals
new
principle
regulating
signaling
suggests
that
functional
architecture
depends
on
assembly
multiprotein
nanodomains
interior
construction
conditional
relationships.
Язык: Английский