Lipid Oxidation at the Crossroads: Oxidative Stress and Neurodegeneration Explored in Caenorhabditis elegans
Antioxidants,
Год журнала:
2025,
Номер
14(1), С. 78 - 78
Опубликована: Янв. 10, 2025
Lipid
metabolism
plays
a
critical
role
in
maintaining
cellular
integrity,
especially
within
the
nervous
system,
where
lipids
support
neuronal
structure,
function,
and
synaptic
plasticity.
However,
this
essential
metabolic
pathway
is
highly
susceptible
to
oxidative
stress,
which
can
lead
lipid
peroxidation,
damaging
process
induced
by
reactive
oxygen
species.
peroxidation
generates
by-products
that
disrupt
many
functions,
with
strong
impact
on
proteostasis.
In
review,
we
explore
of
oxidation
protein
folding
its
associated
pathological
implications,
particular
focus
findings
neurodegeneration
from
Caenorhabditis
elegans
studies,
an
animal
model
remains
underutilized.
Additionally,
highlight
effectiveness
different
methodologies
applied
nematode
deepen
our
understanding
intricate
process.
system
any
animal,
including
mammals
invertebrates,
disturb
delicate
balance
homeostasis,
leading
build-up
toxic
by-products,
misfolding,
key
factors
neurodegenerative
diseases.
This
disruption
contributes
pathogenesis
disorders
such
as
Alzheimer's,
Parkinson's,
or
Huntington's
disease.
The
studies
offer
valuable
insights
into
these
complex
processes
potential
avenues
for
developing
targeted
therapies
mitigate
disease
progression.
Язык: Английский
Temperature-jump microscopy and interaction of Hsp70 heat shock protein with a client protein in vivo
Methods,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 1, 2024
Язык: Английский
A new player in the biogenesis of lysosome-related organelles
The Journal of Cell Biology,
Год журнала:
2024,
Номер
223(10)
Опубликована: Сен. 11, 2024
How
are
Rab
GTPases
regulated
during
lysosome-related
organelle
(LRO)
biogenesis?
Li
et
al.
(https://doi.org/10.1083/jcb.202402016)
identify
LYSMD
proteins
as
crucial
activators
of
Rab32-family
in
LRO
development,
shedding
light
on
the
previously
ambiguous
mechanisms
governing
functionality
this
process.
Язык: Английский
In-cell processing enables rapid and in-depth proteome analysis of low-input Caenorhabditis elegans
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 19, 2024
Abstract
Caenorhabditis
elegans
is
a
widely
used
genetic
model
organism,
however,
the
worm
cuticle
complicates
extraction
of
intracellular
proteins,
prerequisite
for
typical
bottom-up
proteomics.
Conventional
physical
disruption
procedures
are
not
only
time-consuming,
but
can
also
cause
significant
sample
loss,
making
it
difficult
to
perform
proteomics
with
low-input
samples.
Here,
first
time,
we
present
an
on-filter
in-cell
(OFIC)
processing
approach,
which
digest
C.
proteins
directly
in
cells
organism
after
methanol
fixation.
With
OFIC
and
single-shot
LCMS
analysis,
identified
over
9,400
from
200
worms,
largest
proteome
reported
date
that
did
require
fractionation
or
enrichment.
We
systematically
evaluated
performance
approach
by
comparing
conventional
lysis-based
methods.
Our
data
suggest
equivalent
unbiased
identification
quantitation.
further
even
lower
input
samples,
then
this
method
determine
how
impacted
loss
superoxide
dismutase
sod-1
,
ortholog
human
SOD-1
gene
associated
amyotrophic
lateral
sclerosis
(ALS).
Analysis
8,800
50
worms
as
initial
showed
affects
abundance
required
stress
response,
ribosome
biogenesis,
metabolism.
In
conclusion,
our
streamlined
be
broadly
applied
other
systems,
minimizes
while
offering
simplest
workflow
analysis.
Язык: Английский
The neurotrophic factor MANF regulates autophagy and lysosome function to promote proteostasis in Caenorhabditis elegans
Proceedings of the National Academy of Sciences,
Год журнала:
2024,
Номер
121(43)
Опубликована: Окт. 17, 2024
The
conserved
mesencephalic
astrocyte-derived
neurotrophic
factor
(MANF)
is
known
for
protecting
dopaminergic
neurons
and
functioning
in
various
other
tissues.
Previously,
we
showed
that
Язык: Английский
Detecting gene expression in Caenorhabditis elegans
Genetics,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 18, 2024
Abstract
Reliable
methods
for
detecting
and
analyzing
gene
expression
are
necessary
tools
understanding
development
investigating
biological
responses
to
genetic
environmental
perturbation.
With
its
fully
sequenced
genome,
invariant
cell
lineage,
transparent
body,
wiring
diagram,
detailed
anatomy,
wide
array
of
tools,
Caenorhabditis
elegans
is
an
exceptionally
useful
model
organism
linking
cellular
phenotypes.
The
new
techniques
in
recent
years
has
greatly
expanded
our
ability
detect
at
high
resolution.
Here,
we
provide
overview
C.
elegans,
including
transcripts
proteins
situ,
bulk
RNA
sequencing
whole
worms
specific
tissues
cells,
single-cell
sequencing,
high-throughput
proteomics.
We
discuss
important
considerations
choosing
among
these
publicly
available
online
resources
data.
Язык: Английский