A genome-wide association study implicates the olfactory system in Drosophila melanogaster diapause-associated lifespan extension and fecundity DOI Open Access
Sreesankar Easwaran, Denise J. Montell

Опубликована: Окт. 30, 2024

The effects of environmental stress on animal life are gaining importance with climate change. Diapause is a dormancy program that occurs in response to an adverse environment, followed by resumption development and reproduction upon the return favorable conditions. complex trait, so we leveraged Drosophila genetic reference panel (DGRP) lines conducted Genome-Wide Association Study (GWAS) characterize basis diapause. We assessed post-diapause non-diapause fecundity across 193 DGRP lines. GWAS revealed 546 variants, encompassing single nucleotide polymorphisms, insertions deletions associated fecundity. identified 291 candidate diapause-associated genes, 40 which had previously been 89 candidates were more than one SNP. Gene network analysis indicated genes primarily linked neuronal reproductive system development. Similarly, comparison results from other fly greatest overlap olfactory-behavior-associated fecundity-and-lifespan-associated genes. An RNAi screen top two Dip-γ Scribbler, be required during recovery for complemented test neurons successful found although amputation antenna little no effect lifespan, it reduced diapause lifespan postdiapause further show olfactory receptor temperature-sensing Our provide insights into molecular, cellular, adult .

Язык: Английский

Altered circadian rhythm, sleep, and rhodopsin 7 –dependent shade preference during diapause in Drosophila melanogaster DOI Creative Commons

Geoff T. Meyerhof,

Sreesankar Easwaran,

Angela E. Bontempo

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(27)

Опубликована: Июнь 25, 2024

To survive adverse environments, many animals enter a dormant state such as hibernation, dauer, or diapause. Various Drosophila species undergo adult reproductive diapause in response to cool temperatures and/or short day-length. While flies are less active during diapause, it is unclear how environmental conditions affect circadian rhythms and sleep. Here we show that diapause-inducing temperatures, melanogaster exhibit altered activity profiles, including severely reduced morning an advanced evening peak. Consequently, the have single peak at time similar when nondiapausing take siesta. Temperatures ≤15 °C, rather than photoperiod, primarily drive this behavior. At rapidly deep-sleep lacks sleep cycles of higher require high levels stimulation for arousal. Furthermore, 25 prefer siesta shade, preference virtually eliminated 10 °C. Resting shade driven by aversion blue light sensed Rhodopsin 7 outside eyes. Flies °C neuronal markers elevated pressure, increased expression Bruchpilot Ca 2+ R5 ellipsoid body neurons. Therefore, pressure might overcome aversion. Thus, same cause arrest, preserve germline stem cells, extend lifespan, D. prone deep dramatically altered, yet rhythmic, daily patterns.

Язык: Английский

Процитировано

4
A genome-wide association study implicates the olfactory system in Drosophila melanogaster diapause-associated lifespan extension and fecundity DOI Open Access
Sreesankar Easwaran, Denise J. Montell

Опубликована: Фев. 26, 2025

The effects of environmental stress on animal life are gaining importance with climate change. Diapause is a dormancy program that occurs in response to an adverse environment, followed by resumption development and reproduction upon the return favorable conditions. complex trait, so we leveraged Drosophila genetic reference panel (DGRP) lines conducted Genome-Wide Association Study (GWAS) characterize basis diapause. We assessed post-diapause non-diapause fecundity across 193 DGRP lines. GWAS revealed 546 variants, encompassing single nucleotide polymorphisms, insertions deletions associated fecundity. identified 291 candidate diapause-associated genes, 40 which had previously been diapause, 89 were more than one SNP. Gene network analysis indicated genes primarily linked neuronal reproductive system development. Similarly, comparison results from other fly greatest overlap olfactory-behavior-associated fecundity-and-lifespan-associated genes. An RNAi screen selected candidates two Dip-𝛾 Scribbler, be required during recovery for complemented test neurons successful found although amputation antenna little no effect lifespan, it reduced diapause lifespan postdiapause further show olfactory receptor temperature-sensing Our provide insights into molecular, cellular, adult .

Язык: Английский

Процитировано

0
Neuropeptide Dynamics Coordinate Layered Plasticity Mechanisms Adapting Drosophila Circadian Behavior to Changing Environment. DOI Creative Commons
Abhishek Chatterjee,

Joydeep De,

Béatrice Martin

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 18, 2024

Abstract The Drosophila brain contains distinct sets of circadian oscillators responsible for generating the morning and evening bouts locomotor activity, giving rise to a bimodal rest-activity pattern in light-dark cycles. We lack mechanistic understanding how environmental changes reshape this daily profile pattern. Here, we uncover seasonal switch mechanism that remodels bout activity. Under summer-like conditions, an environment favored by fruit flies temperate climates, levels PDF neuropeptide diminish, triggering cascade. Lowered PDFR signaling disinhibits GSK3/SGG advance output. Upon sensing loss, neural activity weakens DN1p-SIF circuit, promoting afternoon rest; leading earlier appearance peak. At same time, functional connections from DN1p LNd strengthen, consequently handing over pacemaker role DN1ps. Taken together, our findings elucidate environment-induced tip balanced output clock network, aligning rhythms with time. Neuropeptide-driven parallel adjustment circuitry protein functioning likely represents conserved strategy across animal species, enabling them adapt their behavior throughout year.

Язык: Английский

Процитировано

1
A genome-wide association study implicates the olfactory system in Drosophila melanogaster diapause-associated lifespan extension and fecundity DOI Open Access
Sreesankar Easwaran, Denise J. Montell

Опубликована: Окт. 30, 2024

The effects of environmental stress on animal life are gaining importance with climate change. Diapause is a dormancy program that occurs in response to an adverse environment, followed by resumption development and reproduction upon the return favorable conditions. complex trait, so we leveraged Drosophila genetic reference panel (DGRP) lines conducted Genome-Wide Association Study (GWAS) characterize basis diapause. We assessed post-diapause non-diapause fecundity across 193 DGRP lines. GWAS revealed 546 variants, encompassing single nucleotide polymorphisms, insertions deletions associated fecundity. identified 291 candidate diapause-associated genes, 40 which had previously been 89 candidates were more than one SNP. Gene network analysis indicated genes primarily linked neuronal reproductive system development. Similarly, comparison results from other fly greatest overlap olfactory-behavior-associated fecundity-and-lifespan-associated genes. An RNAi screen top two Dip-γ Scribbler, be required during recovery for complemented test neurons successful found although amputation antenna little no effect lifespan, it reduced diapause lifespan postdiapause further show olfactory receptor temperature-sensing Our provide insights into molecular, cellular, adult .

Язык: Английский

Процитировано

0