IEEE Reviews in Biomedical Engineering,
Год журнала:
2024,
Номер
18, С. 211 - 230
Опубликована: Дек. 9, 2024
Cardiac
organoids
represent
an
important
bioengineering
opportunity
in
the
development
of
models
to
study
human
heart
pathophysiology.
By
incorporating
multiple
cardiac
cell
types
three-dimensional
culture
and
developmentally-guided
biochemical
signaling,
recapitulate
numerous
features
tissue.
However,
tissue
also
experiences
a
variety
mechanical
forces
as
develops
over
course
each
contraction
cycle.
It
is
now
clear
that
these
impact
cellular
specification,
phenotype,
function,
should
be
incorporated
into
engineering
order
generate
better
models.
In
this
review,
we
discuss
strategies
for
report
effects
organoid
design
on
function
cells.
We
then
environment
heart,
including
arising
from
elasticity,
contraction,
blood
flow,
stretch,
efforts
mimic
biophysical
cues
organoids.
Finally,
review
emerging
areas
research,
development,
formation
multi-organ
models,
simulation
spaceflight
consider
how
investigations
might
benefit
inclusion
cues.
Current Cardiology Reports,
Год журнала:
2025,
Номер
27(1)
Опубликована: Март 20, 2025
Abstract
Purpose
of
Review
This
review
aims
to
explore
recent
advancements
in
bioengineering
approaches
used
developing
and
testing
vitro
cardiac
disease
models.
It
seeks
find
out
how
these
tools
can
address
the
limitations
traditional
models
be
applied
improve
our
understanding
mechanisms,
facilitate
preclinical
drug
screening,
equip
development
personalized
therapeutics.
Recent
Findings
Human
induced
pluripotent
stem
cells
have
enabled
generation
diverse
cell
types
patient-specific
Techniques
like
3D
tissue
engineering,
heart-on-a-chip
platforms,
biomechanical
conditioning,
CRISPR-based
gene
editing
faithful
recreation
complex
microenvironments
conditions.
These
advanced
study
both
genetic
acquired
disorders.
Summary
Bioengineered
are
transforming
basic
science
clinical
research
cardiovascular
by
improving
biomimicry
complexity
analogues,
increasing
throughput
reproducibility
screening
as
well
offering
patient
specificity.
Despite
challenges
scalability
functional
maturity,
integrating
multiple
techniques
with
analytical
modeling
platforms
holds
promise
for
future
precision
medicine
therapeutic
innovations.
Spaceflight
imposes
unique
stressors
that
disrupt
mitochondrial
function,
vital
for
energy
production
and
immune
regulation.
Our
multi-omics
analysis
(proteomics,
bisulfite
sequencing,
RNA-seq,
single-nuclei
RNA/ATAC-seq)
on
astronauts,
rodents,
model
organisms
(flies,
worms,
plants)
revealed
progressive
impairment
of
oxidative
phosphorylation
(OXPHOS)
during
spaceflight,
with
delayed
recovery
post-return
across
species.
In
radiation
≥10.34
mGy
activated
persistent
stress
pathways
multiple
organs
except
in
the
spleen,
older
male
C57BL/6
mice
most
affected.
Astronaut
data
from
NASA
Twins
Study,
JAXA,
Inspiration4
missions
showed
prolonged
dysfunction,
OXPHOS
suppression
TCA
cycle
inhibition
lasting
up
to
82
days.
Bisulfite
sequencing
confirmed
epigenetic
changes
genes.
Lastly,
Kaempferol,
an
antioxidant
activator,
mitigated
radiation-induced
liver
atrophy
preserved
function
human
organoids.
This
cross-species
study
underscores
need
targeted
therapies
protect
biogenesis
long-duration
space
missions.
Antioxidants,
Год журнала:
2025,
Номер
14(2), С. 231 - 231
Опубликована: Фев. 18, 2025
With
the
continued
exploration
of
universe,
there
is
an
increasingly
urgent
need
to
address
health
challenges
arising
from
spaceflight.
In
space,
astronauts
are
exposed
radiation,
confinement
and
isolation,
circadian
rhythm
dysregulation,
microgravity
conditions
that
different
those
on
Earth.
These
risk
factors
jeopardize
astronauts'
health,
thus
affecting
quality
space
missions.
Among
these
factors,
gravitational
changes
influence
balance
between
oxidation
antioxidants,
stimulating
production
reactive
oxygen
species
(ROS),
finally
leading
oxidative
stress
(OS).
OS
leads
damage
biomolecules
such
as
lipids,
proteins,
DNA,
which
causes
development
various
diseases.
The
occurrence
increased
in
affects
multiple
systems,
including
musculoskeletal,
cardiovascular,
nervous,
immune
systems.
this
review,
we
discuss
mechanisms
OS,
physiological
effects
systems
caused
by
environment,
potential
treatments
for
OS.
Finally,
treatment
strategies
summarized,
providing
some
promising
approaches
protecting
future
exploration.
Communications Biology,
Год журнала:
2025,
Номер
8(1)
Опубликована: Май 13, 2025
Current
methods
for
producing
cardiomyocytes
from
human
induced
pluripotent
stem
cells
(hiPSCs)
using
2D
monolayer
differentiation
are
often
hampered
by
batch-to-batch
variability
and
inefficient
purification
processes.
Here,
we
introduce
CM-AI,
a
novel
artificial
intelligence-guided
laser
cell
processing
platform
designed
rapid,
label-free
of
hiPSC-derived
(hiPSC-CMs).
This
approach
significantly
reduces
time
without
the
need
chronic
metabolic
selection
or
antibody-based
sorting.
By
integrating
real-time
cellular
morphology
analysis
targeted
ablation,
CM-AI
selectively
removes
non-cardiomyocyte
populations
with
high
precision.
streamlined
process
preserves
cardiomyocyte
viability
function,
offering
scalable
efficient
solution
cardiac
regenerative
medicine,
disease
modeling,
drug
discovery.
