Physiological Entomology,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 25, 2024
Abstract
Triacylglycerides
(TAGs)
are
neutral
lipids
and
one
of
the
major
energy
storage
forms
in
an
insect
aiding
normal
physiology
including
reproduction
organism.
Importance
TAG
metabolism
gametogenesis
has
been
investigated
insects
like
mosquitoes,
silkworm
Drosophila
.
However,
a
direct
association
between
levels
egg‐to‐adult
viability
not
explored.
In
this
study,
we
have
assessed
both
stored
circulating
melanogaster
lines
with
varying
genetic
backgrounds
established
through
inbreeding
recombinant
strategies.
We
found
noticeable
variation
D.
lines.
Apparently
higher
also
had
viability.
To
validate
this,
analysis
these
variations
supervised
correlation
unsupervised
K‐means
clustering
showed
that
positively
associated
irrespective
gender.
The
findings
suggest
promotes
successful
transition
egg
to
adult.
Taken
together,
only
potential
application
husbandry
ecologically
economically
important
but
holds
importance
translational
research
fertility
vertebrates
humans.
Biotechnology and Bioengineering,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 29, 2024
Cryopreservation
presents
a
critical
challenge
due
to
cryo-damage,
such
as
crystallization
and
osmotic
imbalances
that
compromise
the
integrity
of
biological
tissues
cells.
In
contrast,
various
organisms
in
nature
exhibit
remarkable
freezing
tolerance,
leveraging
complex
molecular
mechanisms
survive
extreme
cold.
This
review
explores
adaptive
strategies
freeze-tolerant
species,
including
regulation
specific
genes,
proteins,
metabolic
pathways,
enhance
survival
low-temperature
environments.
We
then
discuss
recent
advancements
cryopreservation
technologies
aim
mimic
these
natural
phenomena
preserve
cellular
tissue
integrity.
Special
focus
is
given
roles
glucose
metabolism,
microRNA
expression,
cryoprotective
protein
modulation
improving
outcomes.
The
insights
gained
from
studying
antifreeze
offer
promising
directions
for
advancing
techniques,
with
potential
applications
medical,
agricultural,
conservation
fields.
Future
research
should
further
elucidate
develop
more
effective
reliable
methods.
Physiological Entomology,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 25, 2024
Abstract
Triacylglycerides
(TAGs)
are
neutral
lipids
and
one
of
the
major
energy
storage
forms
in
an
insect
aiding
normal
physiology
including
reproduction
organism.
Importance
TAG
metabolism
gametogenesis
has
been
investigated
insects
like
mosquitoes,
silkworm
Drosophila
.
However,
a
direct
association
between
levels
egg‐to‐adult
viability
not
explored.
In
this
study,
we
have
assessed
both
stored
circulating
melanogaster
lines
with
varying
genetic
backgrounds
established
through
inbreeding
recombinant
strategies.
We
found
noticeable
variation
D.
lines.
Apparently
higher
also
had
viability.
To
validate
this,
analysis
these
variations
supervised
correlation
unsupervised
K‐means
clustering
showed
that
positively
associated
irrespective
gender.
The
findings
suggest
promotes
successful
transition
egg
to
adult.
Taken
together,
only
potential
application
husbandry
ecologically
economically
important
but
holds
importance
translational
research
fertility
vertebrates
humans.