
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Фев. 7, 2025
The capability of cells to sense and respond endogenous electrical fields plays a crucial role in processes like nerve regeneration, wound healing, development. In vitro, many cell types by migrating along the corresponding field vectors. This process is known as galvano- or electrotaxis. Here we report on combined impact micro-confinements direct current (dcEFs) motility MDA-MB-231 human breast cancer using self-developed, easy-to-use platform with microchannels ranging from 3 μ m 11 width height. We found that exogenous 100 mV mm -1 1000 altered depending confinement size. Our data show an overall inhibited galvanotaxis confinements, while contrast enhancing effect unconfined found. application not only caused reduction migration speed but also decreased number permeating cells. By applying , single-cell permeation could be prevented confinements 5 smaller.
Язык: Английский